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DNA barcode-based molecular identification system for fish species

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Molecules and Cells

Abstract

In this study, we applied DNA barcoding to identify species using short DNA sequence analysis. We examined the utility of DNA barcoding by identifying 53 Korean freshwater fish species, 233 other freshwater fish species, and 1339 saltwater fish species. We successfully developed a web-based molecular identification system for fish (MISF) using a profile hidden Markov model. MISF facilitates efficient and reliable species identification, overcoming the limitations of conventional taxonomic approaches. MISF is freely accessible at http://bioinfosys.snu.ac.kr:8080/MISF/misf.jsp.

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References

  • Austerlitz, F., David, O., Schaeffer, B., Bleakley, K., Olteanu, M., Leblois, R., Veuille, M., and Laredo, C. (2009). DNA barcode analysis: a comparison of phylogenetic and statistical classification methods. BMC Bioinformatics 10, S10.

    Article  PubMed  Google Scholar 

  • Altschul, S., Gish, W., Miller, W., Myers, E., and Lipman, D. (1990). Basic local alignment search tool. J. Mol. Biol. 215, 403–410.

    CAS  PubMed  Google Scholar 

  • Baker, A., Tavares, E., and Elbourne, R. (2009). Countering criticisms of single mitochondrial DNA gene barcoding in birds. Mol. Ecol. Res. 9, 257–268.

    Article  CAS  Google Scholar 

  • Baldi, P., and Brunak, S. (2001). Bioinformatics: The Machine Learning Approach. The MIT Press.

  • Bensasson, D., Zhang, D., Hartl, D., and Hewitt, G. (2001). Mitochondrial pseudogenes: evolution’s misplaced witnesses. Trends Ecol. Evol. 16, 314–321.

    Article  PubMed  Google Scholar 

  • Breiman, L. (2001). Random forests. Machine Learning 45, 5–32.

    Article  Google Scholar 

  • Eddy, S. (1998). Profile hidden Markov models. Bioinformatics 14, 755–763.

    Article  CAS  PubMed  Google Scholar 

  • Eddy, S. (2008). HMMER user’s guide. Department of Genetics, Washington University School of Medicine 2.

  • Edgar, R. (2004). MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res. 32, 1792–1797.

    Article  CAS  PubMed  Google Scholar 

  • Ekrem, T., Willassen, E., and Stur, E. (2007). A comprehensive DNA sequence library is essential for identification with DNA barcodes. Mol. Phylogenet. Evol. 43, 530–542.

    Article  CAS  PubMed  Google Scholar 

  • Eo, H., Kim, S., Koo, H., and Kim, W. (2009). A machine learning based method for the prediction of G protein-coupled receptor-binding PDZ domain proteins. Mol. Cells 27, 629–634.

    Article  CAS  PubMed  Google Scholar 

  • Folmer, O., Black, M., Hoeh, W., Lutz, R., and Vrijenhoek, R. (1994). DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Mol. Marine Biol. Biotech. 3, 294–299.

    CAS  Google Scholar 

  • France, S., and Hoover, L. (2002). DNA sequences of the mitochondrial COI gene have low levels of divergence among deepsea octocorals (Cnidaria: Anthozoa). Hydrobiologia 471, 149–155.

    Article  Google Scholar 

  • Hajibabaei, M., Janzen, D., Burns, J., Hallwachs, W., and Hebert, P. (2006). DNA barcodes distinguish species of tropical Lepidoptera. Proc. Natl. Acad. Sci. USA 103, 968–971.

    Article  PubMed  Google Scholar 

  • Hebert, P., and Gregory, T. (2005). The promise of DNA barcoding for taxonomy. Systematic Biol. 54, 852–859.

    Article  Google Scholar 

  • Hebert, P., Ratnasingham, S., and deWaard, J. (2003). Barcoding animal life: cytochrome c oxidase subunit 1 divergences among closely related species. Proc. Royal Soc. London-B 270, 96–99.

    Article  Google Scholar 

  • Hebert, P., Stoeckle, M., Zemlak, T., and Francis, C. (2004). Identification of birds through DNA barcodes. PLoS Biol. 2, 1657–1663.

    Article  CAS  Google Scholar 

  • Hubert, N., Hanner, R., Holm, E., Mandrak, N., Taylor, E., Burridge, M., Watkinson, D., Dumont, P., Curry, A., and Bentzen, P. (2008). Identifying Canadian freshwater fishes through DNA barcodes. PLoS ONE 3, e2490.

    Article  Google Scholar 

  • Ihaka, R., and Gentleman, R. (1996). R: A language for data analysis and graphics. J. Comput. Graph. Statistics 5, 299–314.

    Article  Google Scholar 

  • Kimura, M. (1980) A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J. Mol. Evol. 16, 111–120.

    Article  CAS  PubMed  Google Scholar 

  • Lefebure, T., Douady, C., Gouy, M., and Gibert, J. (2006). Relation ship between morphological taxonomy and molecular divergence within Crustacea: proposal of a molecular threshold to help species delimitation. Mol. Phylogenet. Evol. 40, 435–447.

    Article  CAS  PubMed  Google Scholar 

  • Meyer, C., and Paulay, G. (2005). DNA barcoding: error rates based on comprehensive sampling. PLoS Biol. 3, e422.

    Article  PubMed  Google Scholar 

  • Nielsen, R., and Matz, M. (2006). Statistical approaches for DNA barcoding. Systematic Biol. 55, 162–169.

    Article  Google Scholar 

  • Rach, J., DeSalle, R., Sarkar, I., Schierwater, B., and Hadrys, H. (2008). Character-based DNA barcoding allows discrimination of genera, species and populations in Odonata. Proc. Royal Soc. B 275, 237–247.

    Article  CAS  Google Scholar 

  • Ratnasingham, S., and Hebert, P. (2007). BOLD: the Barcode of Life Data System (http://www.barcodinglife.org). Mol. Ecol. Notes 7, 355–364.

    Article  CAS  PubMed  Google Scholar 

  • Rock, J., Costa, F., Walker, D., North, A., Hutchinson, W., and Carvalho, G. (2008). DNA barcodes of fish of the Scotia Sea, Antarctica indicate priority groups for taxonomic and systematics focus. Antarctic Sci. 20, 253–262.

    Article  Google Scholar 

  • Schindel, D., and Miller, S. (2005). DNA barcoding a useful tool for taxonomists. Nature 17, 435.

    Google Scholar 

  • Song, H., Buhay, J., Whiting, M., and Crandall, K. (2008). Many species in one: DNA barcoding overestimates the number of species when nuclear mitochondrial pseudogenes are coamplified. Proc. Natl. Acad. Sci. USA 105, 13486–13491.

    Article  CAS  PubMed  Google Scholar 

  • Stahls, G., Vujic, A., Perez-banon, C., Radenkovic, S., Rojo, S., and Petanidou, T. (2009). COI barcodes for identification of Merodon hoverflies (Diptera, Syrphidae) of Lesvos Island, Greece. Mol. Ecol. Res. 9, 1431–1438.

    CAS  Google Scholar 

  • Steinke, D., Vences, M., Salzburger, W., and Meyer, A. (2005). TaxI: a software tool for DNA barcoding using distance methods. Philos. Transact. B 360, 1975–1980.

    Article  CAS  Google Scholar 

  • Tamura, K., Dudley, J., Nei, M., and Kumar, S. (2007) MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol. Biol. Evol. 24, 1596–1599.

    Article  CAS  PubMed  Google Scholar 

  • Tautz, D., Arctander, P., Minelli, A., Thomas, R., and Vogler, A. (2002). DNA points the way ahead in taxonomy. Nature 418, 479–479.

    Article  CAS  PubMed  Google Scholar 

  • Tavares, E., and Baker, A. (2008) Single mitochondrial gene barcodes reliably identify sister-species in diverse clades of birds. BMC Evol. Biol. 8, 81.

    Article  PubMed  Google Scholar 

  • Ward, R., Hanner, R., and Hebert, P. (2009). The campaign to DNA barcode all fishes, FISH-BOL. J. Fish Biol. 74, 329–356.

    Article  CAS  PubMed  Google Scholar 

  • Waugh, J. (2007). DNA barcoding in animal species: progress, potential and pitfalls. BioEssays 29, 188–197.

    Article  CAS  PubMed  Google Scholar 

  • Woese, C., and Fox, G. (1977). Phylogenetic structure of the prokaryotic domain: the primary kingdoms. Proc. Natl. Acad. Sci. USA 74, 5088–5090.

    Article  CAS  PubMed  Google Scholar 

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Correspondence to Won Kim.

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Kim, S., Eo, HS., Koo, H. et al. DNA barcode-based molecular identification system for fish species. Mol Cells 30, 507–512 (2010). https://doi.org/10.1007/s10059-010-0148-2

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  • DOI: https://doi.org/10.1007/s10059-010-0148-2

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