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Soluble epithin/PRSS14 secreted from cancer cells contains active angiogenic potential

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  • Published:
Molecules and Cells

Abstract

Epithin (PRSS14/matriptase/ST14), a type II membrane protein, is involved in progression of epithelial cancers and metastasis as well as in the normal epidermal barrier function. When activated, it translocates into the cell-cell contacts and sheds into media. In order to understand the specific mechanism during tumor progression, we tested the angiogenic potential of secreted form of epithin. Epithin produced from the cancer cells shed more in hypoxia and induced motility of endothelial cells. Epithin enhanced the migration and invasion of mouse and bovine endothelial cells without cell proliferation. Furthermore, soluble epithin induced endothelial differentiation in the assay of the human endothelial microvessel-like tube formation and in that of the chicken chorioallantoic membrane. The knock-down of epithin in the 427 thymoma cell line abolished the protease activity of secreted epithin fraction, reduced the invasion of endothelial cells through matrigel, and tube formation activity. Only specific antibodies abolished the migration of endothelial cell and the vessel morphogenesis, suggesting that epithin specifically functions in these systems. Therefore, we propose that the secreted epithin in the hypoxic cancer microenvironment plays a role as a proangiogenic factor, and can be modulated with specific antibodies.

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Author notes

  1. Sang Bum Kim

    Present address: Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, TX, 75390, USA

Authors and Affiliations

  1. Department of Biological Sciences, Inha University, Incheon, 402-751, Korea

    Deokjae Lee & Moon Gyo Kim

  2. School of Biological Sciences, Seoul National University, Seoul, 151-747, Korea

    Sang Bum Kim & Dongeun Park

  3. Department of Anatomy and Neurobiology, Biomedical Science Institute, School of Medicine, Kyung Hee University, Seoul, 130-701, Korea

    Joo-Won Jeong

  4. Department of Medicine, University of California, San Diego, La Jolla, California, 92093, USA

    Chungho Kim

Authors
  1. Sang Bum Kim
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  2. Deokjae Lee
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  4. Chungho Kim
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  5. Dongeun Park
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  6. Moon Gyo Kim
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Correspondence to Moon Gyo Kim.

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Kim, S.B., Lee, D., Jeong, JW. et al. Soluble epithin/PRSS14 secreted from cancer cells contains active angiogenic potential. Mol Cells 29, 617–623 (2010). https://doi.org/10.1007/s10059-010-0077-0

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  • DOI: https://doi.org/10.1007/s10059-010-0077-0

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