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Heterologous production of paromamine in Streptomyces lividans TK24 using kanamycin biosynthetic genes from Streptomyces kanamyceticus ATCC12853

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Molecules and Cells

Abstract

The 2-deoxystreptamine and paromamine are two key intermediates in kanamycin biosynthesis. In the present study, pSK-2 and pSK-7 recombinant plasmids were constructed with two combinations of genes: kanABK and kanABKF and kacA respectively from kanamycin producer Streptomyces kanamyceticus ATCC12853. These plasmids were heterologously expressed into Streptomyces lividans TK24 independently and generated two recombinant strains named S. lividans Sk-2/SL and S. lividans SK-7/SL, respectively. ESI/ MS and ESI-LC/MS analysis of the metabolite from S. lividans SK-2/SL showed that the compound had a molecular mass of 163 [M + H]+, which corresponds to that of 2-deoxystreptamine. ESI/MS and MS/MS analysis of metabolites from S. lividans SK-7/SL demonstrated the production of paromamine with a molecular mass of 324 [M + H]+. In this study, we report the production of paromamine in a heterologous host for the first time. This study will evoke to explore complete biosynthetic pathways of kanamycin and related aminoglycoside antibiotics.

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Authors and Affiliations

  1. Sun Moon University, Asan, 336-708, Korea

    Keshav Kumar Nepal, Tae-Jin Oh & Jae Kyung Sohng

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  1. Keshav Kumar Nepal
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  2. Tae-Jin Oh
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  3. Jae Kyung Sohng
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Correspondence to Jae Kyung Sohng.

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Nepal, K.K., Oh, TJ. & Sohng, J.K. Heterologous production of paromamine in Streptomyces lividans TK24 using kanamycin biosynthetic genes from Streptomyces kanamyceticus ATCC12853. Mol Cells 27, 601–608 (2009). https://doi.org/10.1007/s10059-009-0080-5

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  • DOI: https://doi.org/10.1007/s10059-009-0080-5

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