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Fabrication of transplantable human oral mucosal epithelial cell sheets using temperature-responsive culture inserts without feeder layer cells

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Abstract

To exclude bacteria- or animal-derived factors from cultured fabrication of transplantable epithelial cell sheets, primary human oral mucosal epithelial cells were seeded on temperature-responsive culture inserts having submicron-scale pores. Supplying culture medium containing human autologous serum to both apical and basal sides of human epithelial cells allows these cells to grow to confluence. These proliferating cells created stratified epithelial layers even when 3T3 feeder layers and fetal bovine serum were eliminated from culture. Normal keratin expression profiles were obtained with these cells, and basal and midlayer cells expressed p63, a putative stem/progenitor marker. These results suggest that temperature-responsive culture inserts can be useful in clinical settings that require the exclusion of xenogeneic factors.

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Authors and Affiliations

  1. Graduate School of Science and Engineering, Waseda University, Tokyo, Japan

    Daisuke Murakami BSc & Hideo Namiki PhD

  2. Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, 8-1 Kawada-cho, Shinjuku-ku, Tokyo, 162-8666, Japan

    Daisuke Murakami BSc, Masayuki Yamato PhD, Takeshi Ohki MD, PhD, Ryo Takagi MSc, Joseph Yang BSc & Teruo Okano PhD

  3. Department of Ophthalmology and Visual Science, Tohoku University Graduate School of Medicine, Sendai, Japan

    Kohji Nishida MD, PhD

  4. Department of Surgery, Institute of Gastroenterology, Tokyo Women's Medical University, Tokyo, Japan

    Takeshi Ohki MD, PhD

Authors
  1. Daisuke Murakami BSc
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  2. Masayuki Yamato PhD
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  3. Kohji Nishida MD, PhD
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  4. Takeshi Ohki MD, PhD
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  5. Ryo Takagi MSc
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  6. Joseph Yang BSc
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  7. Hideo Namiki PhD
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  8. Teruo Okano PhD
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Corresponding author

Correspondence to Teruo Okano PhD.

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Murakami, D., Yamato, M., Nishida, K. et al. Fabrication of transplantable human oral mucosal epithelial cell sheets using temperature-responsive culture inserts without feeder layer cells. J Artif Organs 9, 185–191 (2006). https://doi.org/10.1007/s10047-006-0342-3

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  • DOI: https://doi.org/10.1007/s10047-006-0342-3

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