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Color Correction of Pathological Images Based on Dye Amount Quantification

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Abstract

Pathological images are color images of stained tissue slides, the color of which varies depending on staining conditions. For reliable diagnosis, the color variation must be corrected in these images. This paper proposes a color correction method for hematoxylin and eosin (H&E) stained pathological images in which the amounts of H&E dyes are estimated based on multispectral imaging technique and Beer Lambert law, and the color image is generated corresponding to the adjusted amount of dyes. This enables us to correct an image to an arbitrary or specified optimal staining-condition image. Through experiments using H&E stained human liver slide images, the effectiveness of the proposed method was confirmed.

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Authors and Affiliations

  1. Imaging Science and Engineering Laboratory, Tokyo Institute of Technology, 4259 Nagatuta, Midori-ku, Yokohama, 226-8503, Japan

    Tokiya Abe & Masahiro Yamaguchi

  2. Frontier Collaborative Research Center, Tokyo Institute of Technology, 4259 Nagatuta, Midori-ku, Yokohama, 226-8503, Japan

    Yuri Murakami & Nagaaki Ohyama

  3. Akasaka Natural Vision Research Center, Telecommunications Advancement Organization of Japan, 1-8-6 Akasaka, Minato-ku, Tokyo, 107-0052, Japan

    Yuri Murakami, Masahiro Yamaguchi & Nagaaki Ohyama

  4. University of Pittsburgh Medical Center Pittsburgh, 5230, Centre Avenue, Pittsburgh, PA, 15232-1381, U.S.A

    Yukako Yagi

Authors
  1. Tokiya Abe
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  2. Yuri Murakami
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  3. Masahiro Yamaguchi
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  4. Nagaaki Ohyama
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  5. Yukako Yagi
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Abe, T., Murakami, Y., Yamaguchi, M. et al. Color Correction of Pathological Images Based on Dye Amount Quantification. OPT REV 12, 293–300 (2005). https://doi.org/10.1007/s10043-005-0293-6

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  • DOI: https://doi.org/10.1007/s10043-005-0293-6

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