Abstract
A new phase contrast microscopy technique for halo reduction is proposed. This technique is based on an apodization method combined with the Zernike phase contrast method. Although it has been a difficult theoretical problem, the proposed technique achieves halo reduction by considering angles of diffraction and phase differences. The technique utilizes an apodized phase plate consisting of a quarter wave phase shift ring with a 25% transmittance, and a pair of adjacent rings, which have 50% transmittance. This element is placed at the back focal plane of the objective. The result is startling, halo reduced images of phase objects providing enhanced inner details.
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Otaki, T. Artifact Halo Reduction in Phase Contrast Microscopy Using Apodization. OPT REV 7, 119–122 (2000). https://doi.org/10.1007/s10043-000-0119-5
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DOI: https://doi.org/10.1007/s10043-000-0119-5