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Prognostic Value of MIB-1, E-Cadherin, and CD44 in Pediatric Chordomas

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Pediatric and Developmental Pathology

Abstract

The prognosis of pediatric chordomas is difficult to predict based on histology. The objective of this study was to assess the expression of a proliferation marker and adhesion molecules in pediatric chordomas and relate the expressions to outcome. In 8 pediatric chordomas, we calculated the MIB-1 labeling index (LI) by counting the number of MIB-1–positive tumor cells in 100 tumor cells. The grade of expression of E-cadherin and CD44 was calculated by estimating the percentage of tumor cells expressing these markers. MIB-1 LI correlated with tumor recurrence (P = 0.007) and low survival rate (P = 0.007). The expression of E-cadherin correlated with disease-free survival (P = 0.009), tumor recurrence (P > 0.0007), and low survival rate (P > 0.0007). CD44 expression did not correlate with recurrence (P = 0.056) or survival rate (P = 0.056). Our results suggest that MIB-1 LI and expression of E-cadherin are helpful to predict outcome in pediatric chordomas.

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References

  1. Sibley RK, Day DL, Dehner LP. Metastasizing chordoma: a pathological and immunohistochemical study with review of the literature. Pediatr Pathol 1987;7:287–301

    CAS  PubMed  Google Scholar 

  2. Abenoza P, Sibley RK. Chordoma: an immunohistological study. Hum Pathol 1986;17:744–747

    Article  CAS  PubMed  Google Scholar 

  3. Miettinen M. Chordoma. Antibodies to epithelial membrane antigen and carcinoembryonic antigen in differential diagnosis. Arch Pathol Lab Med 1984;108:891–924

    CAS  PubMed  Google Scholar 

  4. Horiguchi H, Sano T, Qian ZR, et al. Expression of cell adhesion molecules in chordomas: an immunohistochemical study of 16 cases. Acta Neuropathol 2004;107:91–96

    Article  CAS  PubMed  Google Scholar 

  5. Mori K, Chano T, Kushima R, Hukuda S, Okabe H. Expression of E-cadherin in chordomas: diagnostic marker and possible role of tumor cell affinity. Virchows Arch 2002;440:123–127

    Article  CAS  PubMed  Google Scholar 

  6. Rutherfoord GS, Davies AG. Chordomas—ultrastructure and immunohistochemistry: a report based on the examination of six cases. Histopathology 1987;11:775–787

    Article  CAS  PubMed  Google Scholar 

  7. Miettinen M, Lehto VP, Dahl D, Virtanen I. Differential diagnosis of chordoma, chondroid, an ependymal tumors as aided by anti-intermediate filaments antibodies. Am J Pathol 1983;112:160–169

    CAS  PubMed  PubMed Central  Google Scholar 

  8. Naka T, Oda Y, Iwamoto Y, Shinohara N, Chuman H, Fukui M, Tsuneyoshi M. Immunohistochemical analysis of E-cadherin, alpha-catenin, and neural cell adhesion molecules (NCAM) in chordoma. J Clin Pathol 2001;54:945–950

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Gotz G, Kasper M, Fisher G, Herken R. Intermediate filament typing of the human embryonic and fetal notochord. Cell Tissue Res 1995;280:455–462

    Article  CAS  PubMed  Google Scholar 

  10. Takeichi M. cadherin cell adhesion receptor as a morphologic regulator. Science 1991;251:1451–1455

    Article  CAS  PubMed  Google Scholar 

  11. Behrens J, Mareel MM, Van Roy FM, Birchmeier W. Dissecting tumor cell invasion: epithelial cells acquire invasive properties after the loss of uvomorulin-mediated cell-cell adhesion. J Cell Biol 1989;108:2435–2447

    Article  CAS  PubMed  Google Scholar 

  12. Fahraeus R, Chen W, Trivedi P, Klein G, Obrink B. Decreased expression of E-cadherin and increased invasive capacity in EBV-LMP-transfected human epithelial and murine adenocarcinoma cells. Int J Cancer 1992;52:834–838

    Article  CAS  PubMed  Google Scholar 

  13. Laskin WB, Miettinen M. Epithelial-type and neural-type cadherin expression in malignant noncarcinomatous neoplasms with epithelioid features that involve the soft tissues. Arch Pathol Lab Med 2002;126:425–431

    Article  PubMed  Google Scholar 

  14. Naor D, Nedvetzki S, Golan I, et al. CD44 in cancer. Crit Rev Clin Lab Sci 2002;39:527–579

    Article  CAS  PubMed  Google Scholar 

  15. Turley EA, Noble PW, Bourguignon LY. Signaling properties of hyaluronan receptors. J Biol Chem 2002;277:4589–4592

    Article  CAS  PubMed  Google Scholar 

  16. Guler G, Sarac S, Uner, Karabulut E, Ayhan A, Ogawa H. Prognostic value of CD44 variant 6 in laryngeal epidermoid carcinomas. Arc Otolaryngol Head Neck Surg 2002;128:393–397

    Article  Google Scholar 

  17. Kim HS, Park YB, Oh JH, Jeong J, Kim CJ, Lee SH. Expression of CD44 isoforms correlates with the metastatic potential of osteosarcoma. Clin Orthop 2002;396:184–190

    Article  Google Scholar 

  18. Kawasaki H, Toyada M, Shinohara H, et al. Expression of surviving correlates with apoptosis, proliferation, and angiogenesis during human colorectal tumorigenesis. Cancer 2001;91:2026–2032

    Article  CAS  PubMed  Google Scholar 

  19. Ali-Fehmi R, Qureshi F, Lawrence WD, Jacques SM. Apoptosis, proliferation, and expression of p53 and bcl-2 in endocervical glandular intraepithelial lesions and invasive endocervical adenocarcinoma. Int J Gynecol Pathol 2004;23:1–6

    Article  PubMed  Google Scholar 

  20. Lopez-Beltran A, Luque RJ, Alvarez-Kindelan J, et al. Prognostic factors in stage T1 grade 3 bladder cancer survival: the role of G1-S modulators (p53, p21Waf1, p27kip1, cyclin D1 and cyclin D3) and proliferation index (ki67-MIB1). Eur Urol 2004;45:606–612

    Article  CAS  PubMed  Google Scholar 

  21. Staibano S, Franco R, Tranfa F, et al. Orbital rhabdomyosarcoma: relationship between DNA ploidy, p53, bcl-2, MDR-1 and li67 (MIB1) expression and clinical behavior. Anticancer Res 2004;24:249–257

    CAS  PubMed  Google Scholar 

  22. Roggendorf W, Schuster T, Peiffer J. Proliferative potential of meningiomas determined with the monoclonal antibody Ki-67. Acta Neuropathol 1987;73:361–364

    Article  CAS  PubMed  Google Scholar 

  23. Ho DM, Hsu CY, Ting LT, Chiang H. Histopathology and MIB-1 labeling index predicted recurrence of meningiomas: a proposal of diagnostic criteria for patients with atypical meningioma. Cancer 2002;94:1538–1547

    Article  PubMed  Google Scholar 

  24. Hoyt JW, Grown AM, Kim DK, Berger MS. Analysis of proliferative grade in glial neoplasm using antibodies to the Ki-67 antigen and PCNA in formalin fixed, deparaffinized tissues. J Neurooncol 1995;24:163–169

    Article  CAS  PubMed  Google Scholar 

  25. Frank I, Cheville JC, Blute ML, et al. Prognostic value of p53 and MIB-1 in transitional cell carcinoma of the urinary bladder with regional lymph node involvement. Cancer 2004;101:1803–1808

    Article  PubMed  Google Scholar 

  26. Kilgore S, Prayson RA. Apoptotic and proliferative markers in chordomas: a study of 26 tumors. Ann Diagn Pathol 2002;6:222–228

    Article  PubMed  Google Scholar 

  27. Holton JL, Steel T, Luxsuwong M, Crockard HA, Revesz T. Skull base chordomas: correlation to tumour doubling time with age, mitosis and Ki67 proliferation index. Neuropathol Apl Neurobiol 2000;26:497–503

    Article  CAS  Google Scholar 

  28. Naka T, Boltze C, Samii A, et al. Skull base and nonskull base chordomas: clinicopathologic and immunohistochemical study with special reference to nuclear pleomorphism and proliferative ability. Cancer 2003;98:1934–1941

    Article  PubMed  Google Scholar 

  29. Matsuno A, Sasaki T, Nagashima T, et al. Immunohistochemical examination of proliferative potentials and the expression of cell cycle-related proteins of intracranial chordomas. Hum Pathol 1997;28:714–719

    Article  CAS  PubMed  Google Scholar 

  30. Ariel IM, Verdu C. Chordoma: an analysis of twenty cases treated over a twenty-year span. J Surg Oncol 1975;7:27–44

    Article  CAS  PubMed  Google Scholar 

  31. Congdon CC. Benign and malignant chordomas. A clinicoanatomic study of twenty-two cases. Am J Pathol 1952;28:793–821

    CAS  Google Scholar 

  32. Heffelfinger MJ, Dahlin DC, MacCarty CS, Beabout JW. Chordomas and cartilaginous tumors at the skull base. Cancer 1973;23:410–420

    Article  Google Scholar 

  33. Higinbotham NL, Phillips RF, Farr HW, Hustsu HO. Chordoma. Thirty-five–year study at Memorial Hospital. Cancer 1967;11:1841–1850

    Google Scholar 

  34. Kaneko Y, Sato Y, Iwaki T, Shin RW, Tateishi J, Fukui M. Chordoma in early childhood: a clinicopathological study. Neurosurgery 1991;29:442–446

    Article  CAS  PubMed  Google Scholar 

  35. Tallini G, Dorfman H, Brys P, et al. Correlation between clinicopathological features and karyotype in 100 cartilaginous and chordoid tumours. A report from the chromosme and morphology (CHAMP) collaborative study group. J Pathol 2002;196:194–203

    Google Scholar 

  36. Fletcher CDM, Unni KK, Mertens F (eds). World Health Organization Classification of Tumours. Pathology and Genetics of Tumours of Soft Tissue and Bone. Lyon: IARC Press, 2002

    Google Scholar 

  37. Scheil S, Bruderlein S, Liehr T, et al. Genome-wide analysis of 16 chordomas by comparative genomic hybridization and cytogenetics of the first human chordomas cell line, U-CH1. Genes Chromosomes Cancer 2001;32:203–211

    Article  CAS  PubMed  Google Scholar 

  38. Gil Z, Fliss DM, Voskoboinik N, et al. Cytogenetic analysis of three variants of clival chordoma. Cancer Genet Cytogenet 2004;154:124–130

    Article  CAS  PubMed  Google Scholar 

  39. Hruban RH, Traganos F, Reuter VE, Huvos AG. Chordomas with malignant spindle cell components. A DNA flow cytometric and immunohistochemical study with histogenetic implications. Am J Pathol 1990;137:435–447

    CAS  PubMed  Google Scholar 

  40. Spaar FW, Spaar U, Markakis E. DNA in chordomas of the clivus Blumenbachii. Neurosurg Rev 1990;13:219–229

    Article  CAS  PubMed  Google Scholar 

  41. Berven S, Zurakowski D, Mankin HJ, Gebhardt MC, Springfield DS, Hornicek FJ. Clinical outcome in chordoma: utility of flow cytometry in DNA determination. Spine 2002;27:374–379

    Article  PubMed  Google Scholar 

  42. Ammirati M, Bernardo A. Management of skull base chordoma. Crit Rev Neurosurg 1999;9:63–69

    Article  CAS  PubMed  Google Scholar 

  43. Gay E, Sekhar LN, Rubinstein E, et al. Chordomas and chondrosarcomas of the cranial base: results and follow-up of 60 patients. Neurosurgery 1995;36:887–896

    Article  CAS  PubMed  Google Scholar 

  44. Chambers PW, Schwinn CP. Chordoma: a clinicopathologic study of metastasis. Am J Anat 1979;128:193–224

    Google Scholar 

  45. Forsyth PA, Cascino T, Shaw EG, et al. Intracranial chordomas: a clinicopathological and prognostic study of 51 cases. J Neurosurg 1993;78:741–747

    Article  CAS  PubMed  Google Scholar 

  46. Hug EB, Loredo LN, Slater JD, et al. Proton radiation therapy for chordomas and chondrosarcomas of the skull base. J Neurosurg 1999;91:432–439

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Pathology and Laboratory Medicine, University of Cincinnati College of Medicine, 231 Albert Sabin Way, Cincinnati, OH, 45208-0529, USA

    Aly G. Saad

  2. Division of Pathology and Laboratory Medicine, ML 1010, Cincinnati Children’s Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, OH, 45229-3039, USA

    Margaret H. Collins

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  1. Aly G. Saad
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Correspondence to Margaret H. Collins.

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Saad, A.G., Collins, M.H. Prognostic Value of MIB-1, E-Cadherin, and CD44 in Pediatric Chordomas. Pediatr Dev Pathol 8, 362–368 (2005). https://doi.org/10.1007/s10024-005-1127-z

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  • DOI: https://doi.org/10.1007/s10024-005-1127-z

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