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An In Vitro Model to Compare the Antimicrobial Activity of Silver-Coated Versus Rifampicin-Soaked Vascular Grafts

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Annals of Vascular Surgery

Abstract

In situ replacement of infected vascular grafts is an accepted alternative to total graft excision and extraanatomic replacement. Its success relies upon the ability of the newly inserted graft to resist recurrent infection. This study compares the efficacy of two methods used to reduce the risk of graft reinfection: rifampicin soaking versus silver bonding of grafts. The grafts’ resistance to infection was tested in vitro in two protocols, each using a panel of seven common bacteria, including methicillin-resistant Staphylococcus aureus (MRSA). The length of time the grafts remained free of organisms was compared between the groups. Both the silver graft and the rifampicin-soaked graft were significantly better than control graft at preventing bacterial growth on the graft surface. The rifampicin inhibited the growth of the gram-positive organisms, including MRSA, significantly better than the silver graft on days 2 and 3 (p < 0.001). Conversely, the silver graft was significantly more effective against the gram-negative organisms until day 4 (p < 0.0001). Both types of graft inhibit the in vitro growth of bacteria more effectively than controls, with rifampicin being most effective against gram-positive organisms and silver being best against the gram-negative organisms.

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References

  1. PD Hayes A Nasim NJL London et al. (1999) ArticleTitleRifampicin bonded grafts for the treatment of major aortic graft infection: the Leicester experience J. Vasc. Surg. 30 92–98 Occurrence Handle1:STN:280:DyaK1MzisVSkug%3D%3D Occurrence Handle10394158

    CAS  PubMed  Google Scholar 

  2. AR Naylor PD Hayes S Darke (2001) ArticleTitleA prospective audit of complex wound and graft infections in Great Britain and Ireland: the emergence of MRSA Eur. J. Vasc. Endovasc. Surg. 21 289–294 Occurrence Handle10.1053/ejvs.2001.1311 Occurrence Handle1:STN:280:DC%2BD3M3nsFymtA%3D%3D Occurrence Handle11359327

    Article  CAS  PubMed  Google Scholar 

  3. A Chervu W Moore M Chvapil et al. (1991) ArticleTitleEfficacy and duration of anti-staphylococcal activity comparing 3 antibiotics bonded to Dacron vascular grafts with a collagen release system J. Vasc. Surg. 13 897–901 Occurrence Handle10.1067/mva.1991.27349 Occurrence Handle1:STN:280:By6B2crmsVc%3D Occurrence Handle1828092

    Article  CAS  PubMed  Google Scholar 

  4. PE Magnan P Seyral D Raoult et al. (1994) ArticleTitleIn vitro anti-staphylococcal activity of collagen-sealed Dacron vascular prosthesis bonded with rifampicin, vancomycin or amikacin Ann. Vasc. Surg. 8 243–247 Occurrence Handle1:STN:280:ByuA3MfjvVU%3D Occurrence Handle8043357

    CAS  PubMed  Google Scholar 

  5. CJL Strachan SWB Newsome TR Ashton (991) ArticleTitleThe clinical use of an antibiotic-bonded graft Eur. J. Vasc. Surg. 5 627–632

    Google Scholar 

  6. Mertens, RA, O’Hara, PJ, Hertzer, NR, et al. (1995) “Surgical management of infra-inguinal arterial prosthetic graft infections: review of a thirty-five year experience” J. Vasc. Surg. 21: 782–790

    Google Scholar 

  7. Lorentzen, JE, Nielsen, OM, Arendrup, H, et al. (1985) “Vascualr graft infection: an analysis of sixty-two graft infections in 2411 consecutively implanted synthetic vascular grafts” Surgery 98: 81–86

    Google Scholar 

  8. Edwards, WH, Jr, Martin, RS, III, Jenkins, JM, et al. (1987) “Primary graft infections” J. Vasc. Surg. 6: 235–239

    Google Scholar 

  9. Calligaro, KD, Veith, FJ, Schwartz, ML, et al. (1995) “Recommendations for initial antibiotic treatment of extracavitary arterial graft infections” Am. J. Surg. 170: 123–125

    Google Scholar 

  10. DC Speller AP Johnson D James et al. (1997) ArticleTitleResistance to methicillin and other antibiotics in isolates of S. aureus from blood and CSF, England and Wales, 1989-1995 Lancet 350 323–325 Occurrence Handle10.1016/S0140-6736(97)12148-1 Occurrence Handle1:CAS:528:DyaK2sXls1emur4%3D Occurrence Handle9251636

    Article  CAS  PubMed  Google Scholar 

  11. DF Bandyk ML Novotney BL Johnson et al. (2001) ArticleTitleUse of rifampicin-soaked gelatin-sealed polyester grafts for in situ treatment of primary aortic and vascular prosthetic infections J. Surg. Res. 95 44–49 Occurrence Handle10.1006/jsre.2000.6035 Occurrence Handle1:CAS:528:DC%2BD3cXovVOgtLs%3D Occurrence Handle11120634

    Article  CAS  PubMed  Google Scholar 

  12. G Torsello W Sandman (1997) ArticleTitleUse of antibiotic-bonded grafts in vascular graft infection Eur. J. Vasc. Endovasc. Surg. 14 IssueIDSuppl A 84–87 Occurrence Handle1:STN:280:ByiH28%2Fks1I%3D

    CAS  Google Scholar 

  13. M D’Addato T Curti A Freyrie (1996) ArticleTitleProphylaxis of graft infection with rifampicin-bonded Gelseal graft: two-year follow-up of a prospective clinical trial Cardiovasc. Surg. 4 200–204 Occurrence Handle10.1016/0967-2109(96)82315-5 Occurrence Handle1:STN:280:ByiD3MvmtVA%3D Occurrence Handle8861437

    Article  CAS  PubMed  Google Scholar 

  14. BD Braithwaite B Davies BP Heather et al. (1998) ArticleTitleEarly results of a randomized trial of rifampicin-bonded Dacron grafts for extra-anatomic vascular reconstruction. Joint Vascular Research Group Br. J. Surg. 85 1378–1381 Occurrence Handle10.1046/j.1365-2168.1998.00878.x Occurrence Handle1:STN:280:DyaK1cvltlakuw%3D%3D Occurrence Handle9782018

    Article  CAS  PubMed  Google Scholar 

  15. RO Darouiche (1999) ArticleTitleAnti-infective efficacy of silver-coated medical prostheses Clin. Infect. Dis. 29 1371–1377 Occurrence Handle10.1086/313561 Occurrence Handle1:STN:280:DC%2BD3c%2Fltl2itA%3D%3D Occurrence Handle10585781

    Article  CAS  PubMed  Google Scholar 

  16. JM Schierholz LJ Lucas A Rump G Pulverer (1998) ArticleTitleEfficacy of silver-coated medical devices J. Hosp. Infect. 40 257–262 Occurrence Handle1:STN:280:DyaK1M%2FosFGjtQ%3D%3D Occurrence Handle9868616

    CAS  PubMed  Google Scholar 

  17. SL Mc Hugh RC Merellering CC Hopkins et al. (1975) ArticleTitle Salmonella typhimurium resistant to silver nitrate, chloramphenicol and ampicillin Lancet 1 235–240 Occurrence Handle1:STN:280:CSqD1MbhvFc%3D Occurrence Handle46385

    CAS  PubMed  Google Scholar 

  18. AT Hendry IO Stewart (1979) ArticleTitleSilver-resistant Enterobacteriacae from hospital patients Can. J. Microbiol. 25 915–921 Occurrence Handle1:CAS:528:DyaE1MXltlCktLo%3D Occurrence Handle526888

    CAS  PubMed  Google Scholar 

  19. E Kinney D Bandyk G Seabrook et al. (1991) ArticleTitleAntibiotic-bonded PTFE vascular grafts: the effect of silver antibiotic on bioactivity following implantation J. Surg. Res. 50 430–435 Occurrence Handle1:CAS:528:DyaK3MXksFOitr8%3D Occurrence Handle2038181

    CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Microbiology, Leicester Royal Infirmary, Leicester, UK

    S. Hardman MB, ChB, A. Cope MB, ChB & A. Swann MD

  2. Department of Surgery, Leicester Royal Infirmary, Leicester, UK

    P.R.F. Bell MD, A.R. Naylor MD & P.D. Hayes MD

Authors
  1. S. Hardman MB, ChB
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  2. A. Cope MB, ChB
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  3. A. Swann MD
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  4. P.R.F. Bell MD
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  5. A.R. Naylor MD
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  6. P.D. Hayes MD
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Corresponding author

Correspondence to P.D. Hayes MD.

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Hardman, S., Cope, A., Swann, A. et al. An In Vitro Model to Compare the Antimicrobial Activity of Silver-Coated Versus Rifampicin-Soaked Vascular Grafts. Ann Vasc Surg 18, 308–313 (2004). https://doi.org/10.1007/s10016-004-0042-5

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  • DOI: https://doi.org/10.1007/s10016-004-0042-5

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