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Label-free PSA electrochemical determination by seed-mediated electrochemically-deposited gold nanoparticles on an FTO electrode

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Abstract

A facile and sensitive approach is introduced to precisely determine trace amounts of prostate specific antigen (PSA) by gold nanostructures deposited on fluorine-doped tin oxide (FTO) electrodes. The gold electrode fabrication is carried out by integration of two techniques of vacuum-deposition and electrochemical growth. The electrode was successfully used as a label-free immunosensor for PSA determination in human serum samples. The dependency of the biosensor performance on various fabrication parameters have been investigated and the optimized fabrication rout has been specified. This label-free immunosensor presents a noticeable performance with a large range of linearity from 0.05 to 30 ng/mL, a low detection limit of 5.7 pg/mL, and a long-term stability of 4 weeks. The obtained results for PSA determination in real samples show a good agreement with ELISA results with less than 10% deviation.

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Funding

This work has been supported by the research council of Tarbiat Modares University (TMU). A. M., and H. S. thank the TMU support under the grant number IG-39708.

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Authors and Affiliations

  1. Department of Physics, Tarbiat Modares University, P.O. Box 14115-175, Tehran, Iran

    Maryam Sajadpour, Hossein Siampour & Ahmad Moshaii

  2. Department of Sensor and Biosensor, Faculty of Interdisciplinary Sciences and Technologies, Tarbiat Modares University, P.O Box: 14115-336, Tehran, Iran

    Sara Abbasian & Ahmad Moshaii

  3. Chemical Injuries Research Center, Systems Biology and Poisonings Institute, Baqiyatallah, University of Medical Sciences, Tehran, Iran

    Hasan Bagheri

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  1. Maryam Sajadpour
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  2. Sara Abbasian
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  3. Hossein Siampour
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Correspondence to Ahmad Moshaii.

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Sajadpour, M., Abbasian, S., Siampour, H. et al. Label-free PSA electrochemical determination by seed-mediated electrochemically-deposited gold nanoparticles on an FTO electrode. J Solid State Electrochem 26, 149–161 (2022). https://doi.org/10.1007/s10008-021-05081-y

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  • DOI: https://doi.org/10.1007/s10008-021-05081-y

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