Abstract
The present work proposes a new electrochemical sulfite biosensor based on babassu mesocarp nanoparticles (BMNPs) immobilized on the surface of a pyrolytic graphite electrode (PGE). The synthesized nanoparticles had a size of 121.5 nm and a zeta potential of − 28.1 mV and were used as a support for the immobilization of chitosan (CHIT) and polyphenol oxidase (PPO) obtained from sweet potatoes (Ipomea batatas (L.)). The electrochemical activity of the PGE/BMNPs/CHIT/GA/PPO biosensor was measured using cyclic voltammetry (CV) and square wave voltammetry (SWV), and the biosensor was shown to have a sensitivity of 2.18 μA/μmol L−1, a detection limit of 0.151 μmol L−1, and quantitation limit of 0.452 μmol L−1 for sulfite. The principle of analysis for the developed biosensor is based on the inhibitory effect of sulfite on the activity of the PPO enzyme. This biosensor was successfully employed for the analysis of industrial juice, without the need to pre-treat the sample.
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Acknowledgments
The authors would like to thank the Coordination for the Improvement of Higher Education Personnel (CAPES), National Council for Scientific and Technological Development (CNPq) for the financial support received through the process 431275/2018-1 (Call MCTIC/CNPq No. 28/2018 - Universal/Range B) and the Research Productivity Grant (process 311802/2017-6 (Call CNPq No. 12/2017). Authors also thank to Foundation for Research Support of Piauí (FAPEPI) for their financial support, and the Federal University of Piauí (UFPI) and Federal Institute of Piauí (IFPI) for providing the research and work facilities.
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do Nascimento Marreiro Teixeira, A.S., Teixeira, P.R.S., de Oliveira Farias, E.A. et al. Babassu mesocarp (Orbignya phalerata Mart) nanoparticle-based biosensors for indirect sulfite detection in industrial juices. J Solid State Electrochem 24, 1143–1155 (2020). https://doi.org/10.1007/s10008-020-04546-w
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DOI: https://doi.org/10.1007/s10008-020-04546-w