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A conductive crosslinked graphene/cytochrome c networks for the electrochemical and biosensing study

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Abstract

The direct electrochemistry of catalytically active cytochrome C (Cyt c) adsorbed together with a 3-dimensional network of chemically synthesized graphene on glassy carbon electrode has been readily obtained in aqueous phosphate buffer. Direct electrical communication between the redox center of Cyt c and the modified graphene-based electrode was established. The modified electrode was employed as a high-performance hydrogen peroxide (H2O2) biosensor. The Cyt c present in modified electrode exhibited a pair of quasi-reversible redox peaks with a midpoint potential of −0.380 and −0.2 V, cathodic and anodic, respectively. Investigations into the electrocatalytic activity of the modified electrode upon hydrogen peroxide exposure revealed a rapid amperometric response (5 s). Under optimized conditions, the linear range of response to H2O2 concentration ranged from 5 × 10−7 to 2 × 10−4 M with a detection limit of 2 × 10−7 M at a signal-to-noise ratio of 3. The stability, reproducibility, and selectivity of the proposed biosensor are discussed in relation to the morphology and composition of the modified electrode.

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Acknowledgements

This work was supported by the Fundamental Research Grant Scheme (FRGS) of Malaysia (RDU140122). A.K.M. Kafi acknowledges the Henry Bertie and Florence Mabel Gritton Postdoctoral Fellowship, The University of Sydney.

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Authors and Affiliations

  1. Faculty of Industrial Sciences and Technology, University Malaysia Pahang, 26300, Kuantan, Malaysia

    A. K. M. Kafi & M. M. Yusoff

  2. School of Chemistry, The University of Sydney, Sydney, 2006, Australia

    Mohammad Choucair & Maxwell J. Crossley

Authors
  1. A. K. M. Kafi
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  2. M. M. Yusoff
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  3. Mohammad Choucair
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  4. Maxwell J. Crossley
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Correspondence to A. K. M. Kafi.

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Kafi, A.K.M., Yusoff, M.M., Choucair, M. et al. A conductive crosslinked graphene/cytochrome c networks for the electrochemical and biosensing study. J Solid State Electrochem 21, 2761–2767 (2017). https://doi.org/10.1007/s10008-017-3598-z

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  • DOI: https://doi.org/10.1007/s10008-017-3598-z

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