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Nafion–ZrO2 nanoparticle–ionic liquid nanobiocomposite for the direct electrochemistry of myoglobin

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Abstract

A nanobiocomposite was perpared by mixing zirconia nanoparticle with ionic liquid 1-ethyl-3-methylimidazolium tetrafluoroborate and Nafion together, which was further used to immobilize myoglobin (Mb) on the surface of carbon ionic liquid electrode. UV–Vis and FT-IR spectroscopic results confirmed that Mb remained its native structure in the nanobiocomposite material. Cyclic voltammetric results showed that a pair of well-defined and quasi-reversible redox peaks appeared with the formal potential as −0.219 V (vs. saturated calomel electrode) in pH 7.0 phosphate buffer solution, indicating that the direct electrochemistry of Mb was realized on the modified elecctrode. Mb exhibited a surface-controlled process and the electrochemical parameters were calculated. The Mb electrode showed good electrocatalytic ability to the reduction of trichloroacetic acid within the concentration range from 1.0 to 16.0 mmol/L and the Michaelis–Menten constant was calculated as 7.378 mmol/L. The proposed bioelectrode exhibited good stability and repeatability with the potential application in third-generation biosensor.

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Acknowledgments

We are grateful to the financial support of the Natural Science Foundation of Jiangxi Province (2009GZC0031) and the S&T plan projects of Jiangxi Provincial Education Department (GJJ10590).

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Authors and Affiliations

  1. Jiangxi Key Laboratory of Surface Engineering, Jiangxi Science and Technology Normal University, Jiangxi, 330013, People’s Republic of China

    Chengxiang Ruan, Jin Lou & Weimin Gao

  2. College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, 266042, People’s Republic of China

    Tongtong Li, Xiaomei Ju, Hejian Liu & Wei Sun

Authors
  1. Chengxiang Ruan
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  2. Tongtong Li
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  3. Xiaomei Ju
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  4. Hejian Liu
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  5. Jin Lou
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  6. Weimin Gao
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  7. Wei Sun
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Correspondence to Chengxiang Ruan or Wei Sun.

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Ruan, C., Li, T., Ju, X. et al. Nafion–ZrO2 nanoparticle–ionic liquid nanobiocomposite for the direct electrochemistry of myoglobin. J Solid State Electrochem 16, 3661–3666 (2012). https://doi.org/10.1007/s10008-012-1806-4

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  • DOI: https://doi.org/10.1007/s10008-012-1806-4

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