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Experimental and computational studies indicate the mutation of Glu12 to increase the thermostability of oligomeric protease from Pyrococcus horikoshii

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Abstract

The intracellular protease from Pyrococcus horikoshii (PhpI) is a member of the DJ-1/ThiJ/PfpI superfamily, which is suggested to be involved in cellular protection against environmental stresses. In this study, flexible docking approach was employed to dock the ligand into the active site of PhpI. By analyzing the results, active site architecture and certain key residues responsible for substrate specificity were identified on the enzyme. Our docking result indicates that Glu12 plays an important role in substrate binding. The kinetic experiment conducted by Zhan shows that the E12T mutant is more stable than that of the wild-type. We also predict that Glu15, Lys43, and Tyr46 may be important in the catalytic efficiency and thermostability of enzyme. The new structural and mechanistic insights obtained from computational study should be valuable for detailed structures and mechanisms of the member of the DJ-1 superfamily.

a AAFR-AMC in the a E12T b WT

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Acknowledgments

This work was supported by the National High Technology Program of China (863 Program, 2006A020203) and the basic research fund of Jilin University (No. 200810019). We thank Dr. Dave Case for his kindness in offering us the Amber9 program (amber.scripps.edu) as a freeware.

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Authors and Affiliations

  1. Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, Jilin University, 130023, Changchun, China

    Dongling Zhan, Weiwei Han & Yan Feng

  2. College of Food Science and Engineering, Jilin Agricultural University, 130118, Changchun, China

    Dongling Zhan

Authors
  1. Dongling Zhan
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  2. Weiwei Han
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  3. Yan Feng
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Corresponding authors

Correspondence to Weiwei Han or Yan Feng.

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Weiwei Han and Dongling Zhan contributed equally to this work.

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Zhan, D., Han, W. & Feng, Y. Experimental and computational studies indicate the mutation of Glu12 to increase the thermostability of oligomeric protease from Pyrococcus horikoshii . J Mol Model 17, 1241–1249 (2011). https://doi.org/10.1007/s00894-010-0819-0

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  • DOI: https://doi.org/10.1007/s00894-010-0819-0

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