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Basic study of retinal stem/progenitor cell separation from mouse iris tissue

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Abstract

We described the possibility of retinal regeneration using a novel and efficient technique for culturing and separating retinal stem/progenitor cells from iris tissue. Immunohistochemical staining of adult agouti mouse iris tissue revealed the presence of nestin/low-affinity neurotrophin receptor p75 (p75NTR)-positive cells on the endothelium camerae anterioris side. Cultured mouse iris-derived cells contained little or no melanin and were found to be positive for nestin. Most nestin-positive cells were analyzed for the coexpression of p75NTR as a cell membrane protein. When the p75NTR was used as a marker to sort the cells, we obtained a dense population of nestin-positive cells. Furthermore, the nestin/p75NTR-positive cells were able to differentiate into neural retina cells. Thus, this culture and separation technique is useful for obtaining retinal stem/progenitor cells from adult mouse iris tissue and for the efficient production of neural retina cells.

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Authors and Affiliations

  1. Laboratory of Molecular Biology & Histochemistry, Fujita Health University Joint Research Laboratory, 1-98 Dengakugakubo, Kutsukake-cho, Toyoake, Aichi, 470-1192, Japan

    Naoki Yamamoto

  2. Department of Ophthalmology, Fujita Health University School of Medicine, Aichi, Japan

    Atsuhiro Tanikawa & Masayuki Horiguchi

Authors
  1. Naoki Yamamoto
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  2. Atsuhiro Tanikawa
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  3. Masayuki Horiguchi
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Correspondence to Naoki Yamamoto.

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Yamamoto, N., Tanikawa, A. & Horiguchi, M. Basic study of retinal stem/progenitor cell separation from mouse iris tissue. Med Mol Morphol 43, 139–144 (2010). https://doi.org/10.1007/s00795-009-0486-3

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  • DOI: https://doi.org/10.1007/s00795-009-0486-3

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