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Increased expression of b-defensin-2 and -3 during the development of autoimmune sialoadenitis in MRL/lpr mice

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Abstract

β-Defensins (BD) are small cationic antimicrobial peptides that produced principally in the epithelial cells of a number of organs. The present study analyzed the expression patterns of BD-1, -2, and -3 during the development of sialoadenitis in MRL/lpr mice. Submandibular glands were dissected from MRL/lpr mice at 4, 8, 10, 12, 14, and 16 weeks of age. The expression of mouse (m) BD-1, -2, and -3 mRNAs was examined by RT-PCR and quantified using TaqMan real-time RT-PCR. No significant differences in the level of expression of mBD-1 were observed among mice of different ages. The level of expression of mBD-2 was significantly higher at 16 weeks than at 4 or 8 weeks. The expression level of mBD-3 was highest in 14-week-old mice and was significantly higher than in 4-week or 16-week-old animals. Immunohistochemical staining showed that BD-3 was clearly localized in the ductal cells with variable intensities. In the center of the foci of lymphatic infiltration, ductal staining was faint or often not present. The results indicate that BD-2 and -3 may be upregulated during the development of autoimmune sialoadenitis.

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Authors and Affiliations

  1. Department of Dental Science, Institute of Personalized Medical Science, Health Sciences University of Hokkaido, 2-5 Ainosato, Kita-ku, Sapporo, Hokkaido, 002-8072, Japan

    Masato Saitoh, Daisuke Noro & Yoshihiro Abiko

  2. Department of Pediatric Dentistry, School of Dentistry, Health Sciences University of Hokkaido, Hokkaido, Japan

    Masato Saitoh, Yoshihito Kurashige, Daisuke Noro, Maiko Takeshima & Seiji Igarashi

  3. Department of Oral Pathology, School of Dentistry, Health Sciences University of Hokkaido, Hokkaido, Japan

    Mami Yamazaki, Michiko Nishimura, Sumiko Nakamura, Tohru Kaku & Yoshihiro Abiko

  4. Department of Oral Biochemistry, School of Dentistry, Health Sciences University of Hokkaido, Hokkaido, Japan

    Toshiya Arakawa & Taishin Takuma

  5. Department of Pathophysiology, Tokyo Dental College, Chiba, Japan

    Takashi Inoue

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  1. Masato Saitoh
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  2. Yoshihito Kurashige
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  3. Mami Yamazaki
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  13. Yoshihiro Abiko
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Correspondence to Yoshihiro Abiko.

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Saitoh, M., Kurashige, Y., Yamazaki, M. et al. Increased expression of b-defensin-2 and -3 during the development of autoimmune sialoadenitis in MRL/lpr mice. Med Mol Morphol 40, 157–162 (2007). https://doi.org/10.1007/s00795-007-0375-6

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  • DOI: https://doi.org/10.1007/s00795-007-0375-6

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