Abstract
We report an easy and stable transfection technique using electrogene transfer with a nonviral Epstein–Barr (EB) virus-based vector. To achieve stable transfection of human breast cancer cells, we conducted electrogene transfer of an EB virus-based plasmid vector (reduced size of oriP) containing the enhanced green fluorescence protein (eGFP) gene. Because the EB virus-based vector exhibits high transfer efficiency and strong persistent transgene expression as a result of autonomous replication in human cells, and as Nucleofector electrogene transfer can achieve highly efficient gene transfection, this method is particularly suitable for generation of stably transfected cell lines.
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Shibata, MA., Miwa, Y., Morimoto, J. et al. Easy stable transfection of a human cancer cell line by electrogene transfer with an Epstein–Barr virus-based plasmid vector. Med Mol Morphol 40, 103–107 (2007). https://doi.org/10.1007/s00795-007-0358-7
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DOI: https://doi.org/10.1007/s00795-007-0358-7