Abstract
The function of brush cells (BCs) is unknown. In a previous study, the rat common bile duct was examined by ultrastructural cytochemical methods for localizing HCO3 −, Cl−, and Na+ ions. All ion precipitates increased in or on BCs after secretin or meal stimulation, and it was proposed that BCs may secrete NaHCO3. In this study, immunohistochemical localization of proteins known to be important in HCO3 − secretion was investigated in the rat common bile duct. Immunoreactivity of proteins involved in Cl−/HCO3 − exchange reaction, cystic fibrosis transmembrane conductance regulator (CFTR) and Cl−/HCO3 − exchanger (AE2), was found on the microvilli (MV) and along the basolateral membrane (BLM) of BCs. The proteins involved in HCO3 − production, Na+/HCO3 − cotransporter (NBC), was found along the BLM but was absent on the MV, whereas carbonic anhydrase II (CA II) was observed on the MV and along the BLM. Of proteins responsible for the extrusion of H+, Na+/H+ exchanger 1 (NHE1) was localized along the BLM whereas Na+/H+ exchanger 3 (NHE3) was found on the MV and BLM. Activity of H+/K+-ATPase was found along the BLM and on the MV, and Na+/K+-ATPase was localized along the BLM. The immunoreactivity of most of these proteins was absent or weak in principal cells. These results strongly suggest that BCs are a significant source of HCO3 − secretion.
Similar content being viewed by others
References
JAG Rhodin T Dalhamn (1956) ArticleTitleElectron microscopy of the tracheal ciliated mucosa in rat Z Zellforsch 44 345–412 Occurrence Handle10.1007/BF00345847 Occurrence Handle1:STN:280:CymC3cnit1E%3D Occurrence Handle13353477
T Ogata (2000) ArticleTitleMammalian tuft (brush) cells and chloride cells of other vertebrate share a similar structure and cytochemical reactivities Acta Histochem Cytochem 33 439–449 Occurrence Handle10.1267/ahc.33.439 Occurrence Handle1:CAS:528:DC%2BD3MXivVOgur0%3D
T Ogata (2005) ArticleTitleSodium bicarbonate secretion indicated by ultrastructural cytochemical localization of HCO3 −, Cl−, and Na+ ions on rat bile duct brush cells Med Mol Morphol 38 243–250 Occurrence Handle10.1007/s00795-005-0304-5 Occurrence Handle1:CAS:528:DC%2BD2MXhtlGhsLnF Occurrence Handle16378233
A Sbarbati F Osculati (2005) ArticleTitleA new fate for old cells: brush cells and related elements J Anat 206 349–358 Occurrence Handle10.1111/j.1469-7580.2005.00403.x Occurrence Handle1:STN:280:DC%2BD2M7osVOrtQ%3D%3D Occurrence Handle15817103
VS Elbrønd E Skadhauge L Thomsen V Dantzer (1998) ArticleTitleMorphological adaptations to induced changes in transepithelial sodium transport in chicken lower intestine (coprodeum): a study of resalination, aldosterone stimulation, and epithelial turnover Cell Tissue Res 292 543–552 Occurrence Handle9582411 Occurrence Handle10.1007/s004410051083
T Gomi (1982) ArticleTitleElectron microscopic studies on the alveolar brush cell of the striped snake (Elaphe quadrivirgata) J Med Soc Toho Univ 29 481–489
T Gomi A Kimura H Tsuchiya T Hashimoto K Higashi S Sasa (1987) ArticleTitleElectron microscopic observations of the alveolar brush cell of the bullfrog Zool Sci 4 613–620
AM Isomäki (1973) ArticleTitleA new cell type (tuft cell) in the gastrointestinal mucosa of the rat Acta Path Microbiol Scand Sect A (Suppl) 240 1–35
A Nabeyama CP Leblond (1974) ArticleTitle“Caveolated cells”characterized by deep surface invaginations and abundant filaments in mouse gastro-intestinal epithelia Am J Anat 140 147–166 Occurrence Handle10.1002/aja.1001400203 Occurrence Handle1:STN:280:CSuC2Mnkt1E%3D Occurrence Handle4363601
A Sato S Miyoshi (1997) ArticleTitleFine structure of tuft cells of the main excretory duct epithelium in the rat submandibular gland Anat Rec 248 325–331 Occurrence Handle1:STN:280:ByiA2MjhvFw%3D Occurrence Handle9214549 Occurrence Handle10.1002/(SICI)1097-0185(199707)248:3<325::AID-AR4>3.0.CO;2-O
L Luciano E Reale (1990) ArticleTitleBrush cells of the mouse gallbladder. A correlative light- and electron-microscopical study Cell Tissue Res 262 339–349 Occurrence Handle10.1007/BF00309889 Occurrence Handle1:STN:280:By6C28fisVw%3D Occurrence Handle2076538
D Höfer D Drenckhahn (1998) ArticleTitleIdentification of the taste cell G-protein, α-gustducin, in brush cells of the rat pancreatic duct system Histochem Cell Biol 110 303–309 Occurrence Handle9749964 Occurrence Handle10.1007/s004180050292
I Novak (1990) Electrolyte transport in pancreatic ducts JA Young PYD Wong (Eds) Epithelial secretion of water and electrolytes Springer Heidelberg 239–252
BE Argent RM Case (1994) Pancreatic ducts. Cellular mechanism and control of bicarbonate secretion LR Johnson (Eds) Physiology of the gastrointestinal tract EditionNumber3rd edn. Raven Press New York 1473–1497
CR Marino FS Gorelick (2003) Pancreatic and salivary glands WF Boron EL Boulpaep (Eds) Medical physiology Saunders Philadelphia 908–930
MC Steward H Ishiguro RM Case (2005) ArticleTitleMechanisms of bicarbonate secretion in the pancreatic duct Annu Rev Physiol 67 377–409 Occurrence Handle10.1146/annurev.physiol.67.031103.153247 Occurrence Handle1:CAS:528:DC%2BD2MXjsFeksr0%3D Occurrence Handle15709963
L Luciano S Groos E Real (2003) ArticleTitleBrush cells of rodent gallbladder and stomach epithelia express neurofilaments J Histochem Cytochem 51 187–198 Occurrence Handle1:CAS:528:DC%2BD3sXptVKitw%3D%3D Occurrence Handle12533527
S Hatta J Sakamoto Y Horio (2002) ArticleTitleIon channels and diseases Med Electron Microsc 35 117–126 Occurrence Handle10.1007/s007950200015 Occurrence Handle1:CAS:528:DC%2BD38Xns1ehsb4%3D Occurrence Handle12353132
I Crawford PC Maloney PL Zeitlin WB Guggino SC Hyde H Turley KC Gatter A Harris CF Higgins (1991) ArticleTitleImmunocytochemical localization of the cystic fibrosis gene product CFTR Proc Natl Acad Sci USA 88 9262–9266 Occurrence Handle1:CAS:528:DyaK3MXmsFegt78%3D Occurrence Handle1718002
CR Marino LM Matovcik FS Gorelick JA Cohn (1991) ArticleTitleLocalization of the cystic fibrosis transmembrane conductance regulator in pancreas J Clin Invest 88 712–716 Occurrence Handle1:STN:280:By6A38vltFQ%3D Occurrence Handle1713921
W Zeng MG Lee M Yan J Diaz I Benjamin CR Marino R Kopito S Freedman C Cotton S Muallem P Thomas (1997) ArticleTitleImmuno- and functional characterization of CFTR in submandibular and pancreatic acinar and duct cells Am J Physiol 273 C442–C455 Occurrence Handle1:CAS:528:DyaK2sXlslehs7o%3D Occurrence Handle9277342
NA Ameen T Ardito M Kashgarian CR Marino (1995) ArticleTitleA unique subset of rat and human intestinal villus cells express the cystic fibrosis transmembrane conductance regulator Gastroenterology 108 1016–1023 Occurrence Handle10.1016/0016-5085(95)90198-1 Occurrence Handle1:STN:280:ByqB3M3otFw%3D Occurrence Handle7535272
L Doucet F Mendes T Montier P Delépine D Penque C Férec MD Amaral (2003) ArticleTitleApplicability of different antibodies for the immunohistochemical localization of CFTR in respiratory and intestinal tissues of human and murine origin J Histochem Cytochem 51 1191–1199 Occurrence Handle1:CAS:528:DC%2BD3sXntFentbo%3D Occurrence Handle12923244
E Roussa MF Romero BM Schmitt WF Boron SL Alper F Thévenod (1999) ArticleTitleImmunolocalization of anion exchanger AE2 and Na+-HCO3 − cotransporter in rat parotid and submandibular glands Am J Physiol 277 G1288–G1296 Occurrence Handle1:CAS:528:DC%2BD3cXhs1ygtw%3D%3D Occurrence Handle10600827
E Roussa SL Alper F Thévenod (2001) ArticleTitleImmunolocalization of anion exchanger AE2, Na+/H+ exchangers NHE1 and NHE4, and vacuolar type H+-ATPase in rat pancreas J Histochem Cytochem 49 463–474 Occurrence Handle1:CAS:528:DC%2BD3MXivFKgsbc%3D Occurrence Handle11259449
CR Marino V Jeanes WF Boron BM Schmitt (1999) ArticleTitleExpression and distribution of the Na+-HCO3 − cotransporter in human pancreas Am J Physiol 277 G487–G494 Occurrence Handle1:CAS:528:DyaK1MXls1agu70%3D Occurrence Handle10444464
F Thévenod E Roussa BM Schmitt MF Romero (1999) ArticleTitleCloning and immunolocalization of a rat pancreatic Na+ bicarbonate cotransporter Biochem Biophys Res Commun 264 291–298 Occurrence Handle10527880 Occurrence Handle10.1006/bbrc.1999.1484
H Satoh N Moriyama C Hara H Yamada S Horita M Kunimi K Tsukamoto N Iso-o J Inatomi H Kawakami A Kudo H Endou T Igarashi A Goto T Fujita G Seki (2003) ArticleTitleLocalization of Na+-HCO3 − cotransporter (NBC-1) variants in rat and human pancreas Am J Physiol Cell Physiol 284 C729–C737 Occurrence Handle1:CAS:528:DC%2BD3sXisFegt7k%3D Occurrence Handle12444017
T Kumpulainen P Jalovaara (1981) ArticleTitleImmunohistochemical localization of carbonic anhydrase isoenzymes in the human pancreas Gastroenterology 80 796–799 Occurrence Handle1:CAS:528:DyaL3MXhtlKksLo%3D Occurrence Handle6781974
S Parkkila AK Parkkila T Juvonen H Rajaniemi (1994) ArticleTitleDistribution of the carbonic anhydrase isoenzymes I, II, and VI in the human alimentary tract Gut 35 645–650
SS Spicer ZH Ge RE Tashian DJ Hazen-Martin BA Schulte (1990) ArticleTitleComparative distribution of carbonic anhydrase isozymes III and II in rodent tissues Am J Anat 187 55–64 Occurrence Handle10.1002/aja.1001870107 Occurrence Handle1:CAS:528:DyaK3cXhtFKiu7c%3D Occurrence Handle2105051
GM Lee W Ahn JY Choi X Luo JT Seo PJ Schulthesis GE Shull KH Kim S Muallem (2000) ArticleTitleNa+-dependent transporters mediate HCO3 − salvage across the luminal membrane of the main pancreatic duct J Clin Invest 105 1651–1658 Occurrence Handle1:CAS:528:DC%2BD3cXjvFWmt7g%3D Occurrence Handle10841524 Occurrence Handle10.1172/JCI9207
ME Madden MP Sarras SuffixJr (1987) ArticleTitleDistribution of Na+, K+-ATPase in rat exocrine pancreas as monitored by K+-NPPase cytochemistry and [3H]-ouabain binding: a plasma membrane protein found primarily to be duct cell associated J Histochem Cytochem 35 1365–1374 Occurrence Handle1:CAS:528:DyaL1cXjsFansQ%3D%3D Occurrence Handle2824600
K Iwatsuki A Horiuchi Y Yonekura S Chiba (1989) ArticleTitleInhibitory effect of ouabain and acetazolamide on secretin-stimulated pancreatic exocrine secretion in anaesthetized dog Clin Exp Pharmacol Physiol 16 139–145 Occurrence Handle1:CAS:528:DyaL1MXktVSmsro%3D Occurrence Handle2498020
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Ogata, T. Bicarbonate secretion by rat bile duct brush cells indicated by immunohistochemical localization of CFTR, anion exchanger AE2, Na+/HCO3 − cotransporter, carbonic anhydrase II, Na+/H+ exchangers NHE1 and NHE3, H+/K+-ATPase, and Na+/K+-ATPase. Med Mol Morphol 39, 44–48 (2006). https://doi.org/10.1007/s00795-006-0312-0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s00795-006-0312-0