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Direct proof for the presence and expression of two 5% different 16S rRNA genes in individual cells of Haloarcula marismortui

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Abstract

A single-cell-derived pure culture of Haloarcula marismortui was obtained using the optical tweezers technique. Analysis of the 16S rRNA genes unambiguously demonstrated two 5% different 16S rRNA sequences. In addition, expression of both operons in the same cell was shown by fluorescence in situ hybridization with operon-specific probes.

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Authors and Affiliations

  1. Lehrstuhl für Mikrobiologie, Universität Regensburg, 93053 Regensburg, Germany Tel. +49-941-9433161; Fax +49-941-9432403 e-mail: karl.stetter@biologie.uni-regensburg.de, , , , , , DE

    Gudrun Amann & K. O. Stetter

  2. Max-Planck Institute for Marine Microbiology, Bremen, Germany, , , , , , DE

    Enric Llobet-Brossa, Rudolf Amann & Josefa Antón

Authors
  1. Gudrun Amann
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  2. K. O. Stetter
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  3. Enric Llobet-Brossa
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  4. Rudolf Amann
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  5. Josefa Antón
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Received: September 19, 2000 / Accepted: October 2, 2000

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Amann, G., Stetter, K., Llobet-Brossa, E. et al. Direct proof for the presence and expression of two 5% different 16S rRNA genes in individual cells of Haloarcula marismortui . Extremophiles 4, 373–376 (2000). https://doi.org/10.1007/s007920070007

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  • DOI: https://doi.org/10.1007/s007920070007

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