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An anti-HBV anthraquinone from aciduric fungus Penicillium sp. OUCMDZ-4736 under low pH stress

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Abstract

To obtain new bioactive natural products, the effect of acidic stress on the metabolites of an aciduric fungus was investigated. This fungus, Penicillium sp. OUCMDZ-4736, which was isolated from the sediment around roots of mangrove (Acanthus ilicifolius), produced different compounds and higher yields under pH 2.5 than under neutral conditions. Using spectroscopic analyses and calculations, three new anthraquinone derivatives (1–3) were isolated and identified from the acidic fermentation broth (pH 2.5) of OUCMDZ-4736. Compound 1 showed much stronger anti-hepatitis B virus activity than that of the positive control, lamivudine, strongly inhibiting HBsAg and HBeAg secretion from HepG2.2.15 cells. These results show that extremophiles are a valuable resource of bioactive compounds, and that pH regulation is an effective strategy to induce metabolite production in aciduric fungi.

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Acknowledgements

This work was supported by Grants from the National Natural Science Foundation of China (nos. 81373298, U1501221 and 81561148012).

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Author notes

  1. Yanzheng Jin and Shidong Qin are contributed equally to this work.

Authors and Affiliations

  1. School of Medicine and Pharmacy, Ocean University of China, Qingdao, 266003, China

    Yanzheng Jin, Shidong Qin, Hai Gao, Guoliang Zhu, Wei Wang, Weiming Zhu & Yi Wang

  2. Laboratory for Marine Drugs and Bioproducts, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266003, China

    Yanzheng Jin, Shidong Qin, Hai Gao, Guoliang Zhu, Wei Wang, Weiming Zhu & Yi Wang

Authors
  1. Yanzheng Jin
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  2. Shidong Qin
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  3. Hai Gao
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  4. Guoliang Zhu
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  5. Wei Wang
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  6. Weiming Zhu
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  7. Yi Wang
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Corresponding authors

Correspondence to Weiming Zhu or Yi Wang.

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The authors declare no conflict of interest.

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Communicated by S. Albers.

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Jin, Y., Qin, S., Gao, H. et al. An anti-HBV anthraquinone from aciduric fungus Penicillium sp. OUCMDZ-4736 under low pH stress. Extremophiles 22, 39–45 (2018). https://doi.org/10.1007/s00792-017-0975-6

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  • DOI: https://doi.org/10.1007/s00792-017-0975-6

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