Skip to main content
SpringerLink
Log in

Salinicoccus salsiraiae sp. nov.: a new moderately halophilic gram-positive bacterium isolated from salted skate

  • Original Paper
  • Published:
Extremophiles Aims and scope Submit manuscript

Abstract

Two moderately halophilic low G + C Gram-positive bacteria were isolated from a sample of salted skate (Class Chondrychthyes, Genus Raja). Phylogenetic analysis of the 16S rRNA gene sequence of strains RH1T and RH4 showed that these organisms represented a novel species of the genus Salinicoccus. The new isolates formed pink–red colonies and flocculated in liquid media, with optimum growth in media containing 4% NaCl and pH of about 8.0. These organisms are aerobic but reduce nitrate to nitrite under anaerobic conditions. Acid is produced from several carbohydrates. Oxidase and catalase were detected. Menaquinone 6 was the major respiratory quinone. The major fatty acids of strains RH1T and RH4 were 15:0 anteiso and 15:0 iso. The G + C contents of DNA were 46.2 and 46.0 mol%, respectively. The peptidoglycan was of A3alpha L-Lys-Gly5–6 type. On the basis of the phylogenetic analyses, physiological and biochemical characteristics, we suggest that strain RH1T (=LMG 22840 = CIP 108576) represents a new species of the genus Salinicoccus, for which we propose the name Salinicoccus salsiraiae.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

References

  • Degryse E, Glansdorff N, Pierard A (1978) A comparative analysis of extreme thermophilic bacteria belonging to the genus Thermus. Arch Microbiol 117:189–196

    Article  PubMed  CAS  Google Scholar 

  • Felsenstein J (1993) PHYLIP (phylogenetic inference package), version 3.5.1. Department of Genetics, University of Washington, Seattle

  • Freitas M, Rainey FA, Nobre MF, Silvestre AJD, da Costa MS (2003) Tepidimonas aquatica sp. nov., a new slightly thermophilic β-Proteobacteria isolated from a hot water tank. Syst Appl Microbiol 26:376–381

    Article  PubMed  CAS  Google Scholar 

  • Jukes TH, Cantor CR (1969) Evolution of protein molecules. In: Munro HN (ed) Mammalian protein metabolism. Academic, New York, pp 21–132

    Google Scholar 

  • Maidak BL, Cole JR, Parker CT et al (1999) A new version of the RDP (Ribosomal Database Project). Nucleic Acids Res 27:171–173

    Article  PubMed  CAS  Google Scholar 

  • Marquez MC, Ventosa A, Ruiz-Berraquero F (1990) Marinococcus hispanicus, a new species of moderately halophilic Gram-positive cocci. Int J Syst Bacteriol 40:165–169

    Google Scholar 

  • Mesbah M, Premachandran U, Whitman W (1989) Precise measurement of the G+C content of deoxyribonucleic acid by high performance liquid chromatography. Int J Syst Bacteriol 39:159–167

    CAS  Google Scholar 

  • Moreira C, Rainey FA, Nobre MF, da Silva MT, da Costa MS (2000) Tepidimonas ignava gen. nov., sp. nov., a new chemolithoheterotrophic and slightly thermophilic member of the β-Proteobacteria. Int J Syst Evol Microbiol 50:735–742

    PubMed  CAS  Google Scholar 

  • Nielsen P, Fritze D, Priest FG (1995) Phenetic diversity of alkaliphilic Bacillus strains: proposal for nine new species. Microbiology 141:1745–1761

    Article  CAS  Google Scholar 

  • Nunes OC, Donato MM, da Costa MS (1992) Isolation and characterization of Rhodothermus strains from S. Miguel, Azores. Syst Appl Microbiol 15:92–97

    Google Scholar 

  • Rainey FA, Ward-Rainey N, Kroppenstedt RM, Stackebrandt E (1996) The genus Nocardiopsis represents a phylogenetically coherent taxon and a distinct actinomycete lineage: proposal of Nocardiopsaceae fam. nov. Int J Syst Bacteriol 46:1088–1092

    Article  PubMed  CAS  Google Scholar 

  • Santos MA, Williams RAD, da Costa MS (1989) Numerical taxonomy of Thermus isolates from hot springs in Portugal. Syst Appl Microbiol 12:310–315

    Google Scholar 

  • Schleifer KH (1985) Analysis of the chemical composition and primary structure of murein. Methods Microbiol 18:123–156

    Article  CAS  Google Scholar 

  • Schleifer KH, Kandler O (1972) Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36:407–477

    PubMed  CAS  Google Scholar 

  • Tiago I, Chung AP, Veríssimo A (2004) Bacterial diversity in a nonsaline alkaline environment: heterotrophic aerobic populations. Appl Environ Microbiol 70:7378–7387

    Article  PubMed  CAS  Google Scholar 

  • Tindall BJ (1989) Fully saturated menaquinones in the archaebacterium Pyrobaculum islandicum. FEMS Microbiol Lett 60:251–254

    Article  CAS  Google Scholar 

  • Ventosa A, Quesada E, Rodriguez-Valera F, Ruiz-Berraquero F, Ramos-Cormenzana A (1982) Numerical taxonomy of moderately halophilic Gram-negative Rods. J Gen Microbiol 128:1959–1968

    Google Scholar 

  • Ventosa A, Marquez MC, Ruiz-Berraquero F, Kocur M (1990) Salinicoccus roseus gen. nov., a new moderately halophilic Gram-positive coccus. Syst Appl Microbiol 13:29–33

    Google Scholar 

  • Ventosa A, Marquez MC, Weiss N, Tindall BJ (1992) Transfer of Marinococcus hispanicus to the genus Salinicoccus as Salinicoccus hispanicus comb. nov. Syst Appl Microbiol 15:530–534

    Google Scholar 

  • Zhang W, Xue Y, Ma Y, Zhou P, Ventosa A, Grant WD (2002) Salinicoccus alkaliphilus sp. nov., a novel alkaliphile and moderate halophile from Baer soda lake in inner Mongolia Autonomous Region, China. Int J Syst Evol Microbiol 52:789–793

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

We are indebted to Prof. J. Euzéby (École National Vétérinaire, Toulouse, France) for the etymology of the new organism’s name. We thank Dr Peter Schumann (DSMZ, Germany) for the peptidoglycan analysis.

Author information

Authors and Affiliations

  1. Departamento de Bioquímica, Centro de Neurociências e Biologia Celular, Universidade de Coimbra, 3001-401, Coimbra, Portugal

    Luís França & Milton S. da Costa

  2. Department of Biological Sciences, Louisiana State University, Baton Rouge, LA, 70803, USA

    Fred A. Rainey

  3. Departamento de Zoologia, Universidade de Coimbra, 3004-517, Coimbra, Portugal

    M. Fernanda Nobre

Authors
  1. Luís França
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Fred A. Rainey
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. Fernanda Nobre
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Milton S. da Costa
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Milton S. da Costa.

Additional information

Communicated by G. Antranikian

Rights and permissions

Reprints and permissions

About this article

Cite this article

França, L., Rainey, F.A., Nobre, M.F. et al. Salinicoccus salsiraiae sp. nov.: a new moderately halophilic gram-positive bacterium isolated from salted skate. Extremophiles 10, 531–536 (2006). https://doi.org/10.1007/s00792-006-0532-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00792-006-0532-1

Keywords

Search

Navigation