Abstract
Shewanella sp. Ac10 is a psychrotrophic bacterium isolated from the Antarctica that actively grows at such low temperatures as 0°C. Immunoblot analyses showed that a heat-shock protein DnaK is inducibly formed by the bacterium at 24°C, which is much lower than the temperatures causing heat shock in mesophiles such as Escherichia coli. We found that the Shewanella DnaK (SheDnaK) shows much higher ATPase activity at low temperatures than the DnaK of E. coli (EcoDnaK): a characteristic of a cold-active enzyme. The recombinant SheDnaK gene supported neither the growth of a dnaK-null mutant of E. coli at 43°C nor λ phage propagation at an even lower temperature, 30°C. However, the recombinant SheDnaK gene enabled the E. coli mutant to grow at 15°C. This is the first report of a DnaK supporting the growth of a dnaK-null mutant at low temperatures.
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Acknowledgements
This work was supported in part by the Grant-in-Aid for Scientific Research 09460049 (to N.E.), and Grant-in-Aid for Scientific Research on Priority Areas (B) 13125203 (to N.E.) from the Ministry of Education, Culture, Sports, Science, and Technology, and by the Pioneering Research Project (to T.Y.) in Biotechnology of the Ministry of Agriculture, Forestry, and Fisheries.
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Yoshimune, K., Galkin, A., Kulakova, L. et al. Cold-active DnaK of an Antarctic psychrotroph Shewanella sp. Ac10 supporting the growth of dnaK-null mutant of Escherichia coli at cold temperatures. Extremophiles 9, 145–150 (2005). https://doi.org/10.1007/s00792-004-0429-9
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DOI: https://doi.org/10.1007/s00792-004-0429-9