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Natronolimnobius baerhuensis gen. nov., sp. nov. and Natronolimnobius innermongolicus sp. nov., novel haloalkaliphilic archaea isolated from soda lakes in Inner Mongolia, China

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Abstract

Three novel isolates of haloalkaliphilic archaea, strains IHC-005T, IHC-010, and N-1311T, from soda lakes in Inner Mongolia, China, were characterized to elucidate their taxonomic positions. The three strains were aerobic, Gram-negative chemoorganotrophs growing optimally at 37–45°C, pH 9.0–9.5, and 15–20% NaCl. Cells of strains IHC-005T/IHC-010 were motile rods, while those of strain N-1311T were non-motile pleomorphic flats or cocci. The three strains contained diphytanyl and phytanyl-sesterterpanyl diether derivatives of phosphatidylglycerol and phosphatidylglycerophosphate methyl ester. No glycolipids were detected. On phylogenetic analysis of 16S rRNA gene sequences, they formed an independent cluster in the Natro group of the family Halobacteriaceae. Comparison of their morphological, physiological, and biochemical properties, DNA G+C content and 16S rRNA gene sequences, and DNA-DNA hybridization study support the view that strains IHC-005T/IHC-010 and strain N-1311T represent separate species. Therefore, we propose Natronolimnobius baerhuensis gen. nov., sp. nov. for strains IHC-005T (=CGMCC 1.3597T =JCM 12253T)/IHC-010 (=CGMCC 1.3598=JCM 12254) and Natronolimnobius innermongolicus sp. nov. for N-1311T (=CGMCC 1.2124T =JCM 12255T).

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Acknowledgements

We thank to Prof. You-xin Song for arranging the sampling trip to Hailar, China, and Dr. Huawei Xin for providing the preliminary taxonomic data of strain N-1311T.

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Authors and Affiliations

  1. Japan Collection of Microorganisms, RIKEN BioResource Center, Wako, Saitama, 351-0198, Japan

    Takashi Itoh & Takashi Yamaguchi

  2. Faculty of Life Sciences, Toyo University, Itakura, Gunma, 374-0193, Japan

    Takashi Yamaguchi & Tomonori Takashina

  3. The Institute of Microbiology, Chinese Academy of Sciences (CAS), Beijing, 100080, P.R. China

    Peijin Zhou

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  1. Takashi Itoh
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  2. Takashi Yamaguchi
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  3. Peijin Zhou
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  4. Tomonori Takashina
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Correspondence to Takashi Itoh.

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Communicated by W.D. Grant

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Itoh, T., Yamaguchi, T., Zhou, P. et al. Natronolimnobius baerhuensis gen. nov., sp. nov. and Natronolimnobius innermongolicus sp. nov., novel haloalkaliphilic archaea isolated from soda lakes in Inner Mongolia, China. Extremophiles 9, 111–116 (2005). https://doi.org/10.1007/s00792-004-0426-z

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  • DOI: https://doi.org/10.1007/s00792-004-0426-z

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