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Bacterial diversity of the Inner Mongolian Baer Soda Lake as revealed by 16S rRNA gene sequence analyses

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Abstract

Bacterial diversity associated with Baer Soda Lake in Inner Mongolia of China was investigated using a culture-independent method. Bacterial 16S rRNA gene libraries were generated using bacterial oligonucleotide primers, and 16S rRNA gene sequences of 58 clones were analyzed phylogenetically. The library was dominated by 16S rDNAs of Gram-negative bacteria (24% α-Proteobacteria, 31% β-Proteobacteria, 33% γ-Proteobacteria, and 2% δ-Proteobacteria), with a lower percentage of clones corresponding to Gram-positive bacteria. Forty cloned sequences were similar to that of known bacterial isolates (>97% sequence similarity), represented by the species of the genera Brevundimonas, Comamonas, Alcaligenes, Stenotrophomonas, and Klebsiella. Eighteen cloned sequences showed less affiliation with known taxa (<97% sequence similarity) and may represent novel taxa.

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Acknowledgments

This work was supported by a grant from the Ministry of Science and Technology of China and a grant from Chinese Academy of Sciences.

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Authors and Affiliations

  1. Department of Microbial Physiology and Ecology, Institute of Microbiology, Chinese Academy of Sciences, 100080, Beijing , China

    Yanhe Ma, Weizhou Zhang, Yanfen Xue & Peijin Zhou

  2. Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Sevilla, 41012 , Sevilla, Spain

    Antonio Ventosa

  3. Department of Microbiology and Immunology, University of Leceister, Leceister , LE1 9HN, UK

    William D. Grant

Authors
  1. Yanhe Ma
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  2. Weizhou Zhang
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  3. Yanfen Xue
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  4. Peijin Zhou
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  5. Antonio Ventosa
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  6. William D. Grant
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Correspondence to Yanhe Ma.

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Communicated by K. Horikoshi

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Ma, Y., Zhang, W., Xue, Y. et al. Bacterial diversity of the Inner Mongolian Baer Soda Lake as revealed by 16S rRNA gene sequence analyses. Extremophiles 8, 45–51 (2004). https://doi.org/10.1007/s00792-003-0358-z

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  • DOI: https://doi.org/10.1007/s00792-003-0358-z

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