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In vitro evaluation of the sealing ability of combined use of iRoot BP Plus and iRoot SP for root-end filling

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Abstract

Objectives

The aim of this in vitro study was to evaluate the sealing ability of combined application of iRoot BP Plus Root Repair Material (BP-RRM) and iRoot SP Injectable Root Canal Sealer (SP-RCS) for root-end filling.

Material and methods

A total of 120 extracted human teeth were used in this study and were randomly divided into four groups. The BP-RRM+SP-RCS group included teeth retro-filled with combined use of BP-RRM and SP-RCS (n=45), and the BP-RRM group included teeth retro-filled by BP-RRM alone (n=45). Teeth without root-end preparation and filling were equally divided into positive control (n=15) and negative control (n=15). The apical sealing ability was evaluated by micro-CT analysis, dye penetrant examination, bacterial leakage test, and glucose leakage test.

Results

Micro-CT analysis showed that the total void fraction of BP-RRM+SP-RCS group was significantly lower than that of BP-RRM group, particularly at the coronal 1/3 segment of the retro-filled roots. Consistently, the maximum linear depth of dye leakage in BP-RRM+SP-RCS group was less than that of BP-RRM group. Bacterial leakage test showed that the microbial leakage in BP-RRM+SP-RCS group was significantly less than that in BP-RRM group. However, no significant difference in glucose leakage between BP-RRM+SP-RCS group and BP-RRM group was observed.

Conclusion

Combined use of BP-RRM and SP-RCS for root-end filling promotes apical sealing in vitro.

Clinical relevance

Combined use of BP-RRM and SP-RCS for root-end filling exhibited better apical sealing as compared to BP-RRM alone in vitro, and this may help reducing technical sensitivity and promoting clinical efficiency during endodontic microsurgery.

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Funding

This work was supported by a grant from the Science & Technology Department of Sichuan Province (2021YFQ0064).

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Authors and Affiliations

  1. State Key Laboratory of Oral Diseases and National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China

    Xu Dong & Xin Xu

  2. Department of Cariology and Endodontics, West China Hospital of Stomatology, Sichuan University, Chengdu, China

    Xu Dong & Xin Xu

  3. Department of Endodontics, College of Dentistry, University of Illinois at Chicago, Chicago, USA

    Qian Xie

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Contributions

X.X. conceived the ideas; X.D. performed all the experimental procedures and analyzed the data; X.D., Q.X., and X.X. managed the writing of the manuscript. All the authors reviewed the manuscript critically.

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Correspondence to Xin Xu.

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This study was approved by the Ethics Committee of the West China Hospital of Stomatology, Chengdu, China (WCHSIRB-D-2022-202).

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Informed consent was obtained from the patients who had teeth extracted for orthodontic reasons.

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Dong, X., Xie, Q. & Xu, X. In vitro evaluation of the sealing ability of combined use of iRoot BP Plus and iRoot SP for root-end filling. Clin Oral Invest 27, 2969–2977 (2023). https://doi.org/10.1007/s00784-023-04896-5

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