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The effectiveness of hydroxyapatite-beta tricalcium phosphate incorporated into stem cells from human exfoliated deciduous teeth for reconstruction of rat calvarial bone defects

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Abstract

Objective

To investigate the effects of stem cells from the pulp of human exfoliated deciduous teeth (SHED) on biphasic calcium phosphate granules (BCP) to repair rat calvarial defects as compared to autogenous bone grafting.

Materials and methods

A defect with a 6-mm diameter was produced on the calvaria of 50 rats. BCP granules were incorporated into SHED cultures grown for 7 days in conventional (CM) or osteogenic (OM) culture media. The animals were allocated into 5 groups of 10, namely: clot, autogenous bone, BCP, BCP+SHED in CM (BCP-CM), and BCP+SHED in OM (BCP-OM). The presence of newly formed bone and residual biomaterial particles was assessed by histometric analysis after 4 and 8 weeks.

Results

The autogenous group showed the largest newly formed bone area at week 8 and in the entire experimental period, with a significant difference in relation to the other groups (P < 0.05). At week 8, BCP-CM and BCP-OM groups showed homogeneous new bone formation (P = 0.13). When considering the entire experimental period, the BCP group had the highest percentage of residual particle area, with no significant difference from the BCP-CM group (P = 0.06) and with a significant difference from the BCP-OM group (P = 0.01). BCP-CM and BCP-OM groups were homogeneous throughout the experimental period (P = 0.59).

Conclusions

BCP incorporated into SHED cultures showed promising outcomes, albeit less pronounced than autogenous grafting, for the repair of rat calvarial defects.

Clinical relevance

BCP incorporated into SHED cultures showed to be an alternative in view of the disadvantages to obtain autogenous bone graft.

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Funding

This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Coordination for the Improvement of Higher Education Personnel) - Brasil (CAPES) - Finance Code 001.

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Authors and Affiliations

  1. Faculty of Dentistry, Department of Clinic and Surgery, Federal University of Alfenas-MG, Rua Gabriel Monteiro da Silva, 700 – 37130-001, Cenro, Alfenas, MG, Brazil

    Alexandre Augusto Ferreira da Silva, Ugo Guilherme Roque Rinco, Vivien Thiemy Sakai & Ronaldo Célio Mariano

  2. Faculty of Dentistry, José do Rosário Vellano University, Rodovia MG-179 Km 0, s/n -37130-000, Bairro Trevo, Alfenas, MG, Brazil

    Ricardo Garcia Mureb Jacob

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  1. Alexandre Augusto Ferreira da Silva
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Correspondence to Alexandre Augusto Ferreira da Silva.

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This article contains studies with animals performed by any of the authors. All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. This study was approved by the Committee on Ethics in the Use of Animals (CEUA-Unifal-MG) under the number 32/2019. For this type of study, formal consent is not required.

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da Silva, A.A.F., Rinco, U.G.R., Jacob, R.G.M. et al. The effectiveness of hydroxyapatite-beta tricalcium phosphate incorporated into stem cells from human exfoliated deciduous teeth for reconstruction of rat calvarial bone defects. Clin Oral Invest 26, 595–608 (2022). https://doi.org/10.1007/s00784-021-04038-9

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