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The consumption of milk supplemented with probiotics decreases the occurrence of caries and the salivary concentration of hβD-3 in children

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Abstract

Objectives

This study evaluated the effect of milk supplemented with Lactobacillus rhamnosus SP1 on the occurrence of caries and the salivary concentration of human β-defensin-3 (hβD-3) in preschool children with high caries risk.

Materials and methods

A sample of 42 children was randomly assigned to two groups; children in the intervention group were given 150 mL of milk supplemented with 107 CFU/mL of Lactobacillus rhamnosus SP1, while children in the control group were given standard milk, for 10 months. The occurrence of dental caries was assessed using the International Caries Detection and Assessment System (ICDAS), and the concentration of hβD-3 was measured in unstimulated saliva using an ELISA test at baseline and after the intervention.

Results

There was an increase in the number of teeth with carious lesions (dICDAS2-6 mft) in the control group, and this increase was statistically significant (p = 0.0489). The concentration of hβD-3 in saliva from the intervention group decreased from 597.91 to 126.29 pg/mL (p = 0.0061), unlike in the control group, where no change in hβD-3 salivary concentration was found.

Conclusions

These findings showed that regular intake of probiotic-supplemented milk in preschool children with high caries risk decreased the occurrence of caries and the salivary levels of hβD-3.

Clinical relevance

Our results suggest the need for developing and implementing probiotic supplementation, as adjuvants to the conventional treatments for caries and allow to considerate the salivary levels of hβD-3 as markers of oral tissue homeostasis.

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Funding

The work was supported by the Grant FIOUCH 17/011 from Dirección de Investigación, Facultad de Odontología, Universidad de Chile.

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Authors and Affiliations

  1. Department of Restorative Dentistry, Dentistry Faculty, University of Chile, Santiago, Chile

    Francisca Sandoval, Simone Faleiros, Rodrigo Cabello & Gonzalo Rodríguez

  2. Institute of Research in Dental Sciences, Dentistry Faculty, University of Chile, Olivos 943, 8380492, Santiago, Metropolitan Region, Chile

    Mario Díaz-Dosque & Alejandro Escobar

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  1. Francisca Sandoval
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  2. Simone Faleiros
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Correspondence to Alejandro Escobar.

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All procedures performed in studies involving human participants were in accordance with the ethical standards of the ethics committee of the Faculty of Dentistry at the University of Chile (certificate no. 2011/14) and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

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Sandoval, F., Faleiros, S., Cabello, R. et al. The consumption of milk supplemented with probiotics decreases the occurrence of caries and the salivary concentration of hβD-3 in children. Clin Oral Invest 25, 3823–3830 (2021). https://doi.org/10.1007/s00784-020-03712-8

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