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Levels of growth factors from platelet-rich fibrin from chronic periodontitis versus periodontally healthy subjects: a pilot study

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Abstract

Objectives

This study aimed to (1) compare the amounts of growth factors from platelet-rich fibrin (PRF) between chronic periodontitis and periodontally healthy subjects and (2) evaluate the relationships between the amounts of growth factors from PRF with complete blood counts (white blood cell (WBC) and platelet counts) and the serum concentrations of IL-1β, IL-6, and tumor necrosis factor-α (TNF-α).

Materials and methods

Venous blood was collected from chronic periodontitis (test) and periodontally healthy subjects (control). PRF and serum were collected from the centrifuged blood. Liquid exudates from the compression of PRF were collected. The compressed PRF membranes were incubated in saline, and eluted aliquots were collected at 1, 24, and 72 h, and the membranes were then digested with trypsin. Epidermal growth factor, insulin-like growth factor-1, platelet-derived growth factor-BB, transforming growth factor-β1, and vascular endothelial growth factor in the exudates and eluents were quantified by ELISA. Serum was used for IL-1β, IL-6, and TNF-α quantifications. Complete blood counts were measured.

Results

There were no significant differences in the amounts of growth factors from PRF exudates and membranes measured between groups (all p > 0.05). The test group had significantly higher WBC (p < 0.05). However, there was no significant correlation between the WBC and the amounts of the growth factors from PRF (all p > 0.05).

Conclusions

PRF can be utilized as an autologous source of growth factors not affected by periodontal condition and WBC level.

Clinical relevance

The amounts of growth factors from PRF were not affected by the periodontal condition of the patient.

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References

  1. Eke P, Dye B, Wei L, Thornton-Evans G, Genco R (2012) Prevalence of periodontitis in adults in the United States: 2009 and 2010. J Dent Res 91:914–920

    Article  Google Scholar 

  2. Górska R, Gregorek H, Kowalski J, Laskus-Perendyk A, Syczewska M, Madaliński K (2003) Relationship between clinical parameters and cytokine profiles in inflamed gingival tissue and serum samples from patients with chronic periodontitis. J Clin Periodontol 30:1046–1052

    Article  Google Scholar 

  3. Avila-Ortiz G, De Buitrago JG, Reddy MS (2015) Periodontal regeneration–furcation defects: a systematic review from the AAP regeneration workshop. J Periodontol 86(suppl.:S108–S130

    Article  Google Scholar 

  4. Kao RT, Nares S, Reynolds MA (2015) Periodontal regeneration–intrabony defects: a systematic review from the AAP regeneration workshop. J Periodontol 86 (suppl.:S77–S104

    Article  Google Scholar 

  5. Choukroun J, Diss A, Simonpieri A, Girard M, Schoeffler C, Dohan SL, Dohan AJJ, Mouhyi J, Dohan D (2006) Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part IV: clinical effects on tissue healing. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 101:e56–e60

    Article  Google Scholar 

  6. Choukroun J, Diss A, Simonpieri A, Girard M, Schoeffler C, Dohan SL, Dohan AJJ, Mouhyi J, Dohan D (2006) Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part V: histologic evaluations of PRF effects on bone allograft maturation in sinus lift. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 101:299–303

    Article  Google Scholar 

  7. Dohan DM, Choukroun J, Diss A, Dohan SL, Dohan AJJ, Mouhyi J, Gogly B (2006) Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part I: technological concepts and evolution. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 101:e37–e44

    Article  Google Scholar 

  8. Dohan DM, Choukroun J, Diss A, Dohan SL, Dohan AJJ, Mouhyi J, Gogly B (2006) Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part II: platelet-related biologic features. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 101:e45–e50

    Article  Google Scholar 

  9. Dohan DM, Choukroun J, Diss A, Dohan SL, Dohan AJJ, Mouhyi J, Gogly B (2006) Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part III: leucocyte activation: a new feature for platelet concentrates? Oral Surg Oral Med Oral Pathol Oral Radiol Endod 101:e51–e55

    Article  Google Scholar 

  10. Kobayashi E, Fluckiger L, Fujioka-Kobayashi M, Sawada K, Sculean A, Schaller B, Miron RJ (2016) Comparative release of growth factors from PRP, PRF, and advanced-PRF. Clin Oral Investig 20:2353–2360

    Article  Google Scholar 

  11. Masuki H, Okudera T, Watanebe T, Suzuki M, Nishiyama K, Okudera H, Nakata K, Uematsu K, Su C, Kawase T (2016) Growth factor and pro-inflammatory cytokine contents in platelet-rich plasma (PRP), plasma rich in growth factors (PRGF), advanced platelet-rich fibrin (A-PRF), and concentrated growth factors (CGF). Int J Implant Dent 2:19

    Article  Google Scholar 

  12. Singh A, Kohli M, Gupta N (2012) Platelet rich fibrin: a novel approach for osseous regeneration. J Maxillofac Oral Surg 11:430–434

    Article  Google Scholar 

  13. Sharma A, Pradeep AR (2011) Autologous platelet-rich fibrin in the treatment of mandibular degree II furcation defects: a randomized clinical trial. J Periodontol 82:1396–1403

    Article  Google Scholar 

  14. Thorat M, Pradeep AR, Pallavi B (2011) Clinical effect of autologous platelet-rich fibrin in the treatment of intra-bony defects: a controlled clinical trial. J Clin Periodontol 38:925–932

    Article  Google Scholar 

  15. Miron RJ, Dham A, Dham U, Pikos MA, Sculean A (2018) The effect of age, gender, and time between blood draw and start of centrifugation on the size outcomes of platelet-rich fibrin (PRF) membranes. Clin Oral Investig 2:1–7

    Google Scholar 

  16. Larjava H (2012) Oral wound healing: cell biology and clinical management, 1st edn. John Wiley & Sons, pp 261–286

  17. Sabharwal A, Gomes-Filho IS, Stellrecht E, Scannapieco FA (2018) Role of periodontal therapy in management of common complex systemic diseases and conditions: an update. Periodontol 78:212–226

    Article  Google Scholar 

  18. AAP, American Academy of Periodontology Task Force (2015) American Academy of Periodontology task force report on the update to the 1999 classification of periodontal diseases and conditions. J Periodontol 86:835–838

  19. Smith PC, Martínez C, Cáceres M, Martínez J (2015) Research on growth factors in periodontology. Periodontol 67:234–250

    Article  Google Scholar 

  20. Choukroun J, Ghanaati S (2018) Reduction of relative centrifugation force within injectable platelet-rich-fibrin (PRF) concentrates advances patients’ own inflammatory cells, platelets and growth factors: the first introduction to the low speed centrifugation concept. Eur J Trauma Emerg Surg 44:87–95

    Article  Google Scholar 

  21. Flemmig TF (1999) Periodontitis. Ann Periodontol 4:32–37

    Article  Google Scholar 

  22. Ehrenfest DMD, Rasmusson L, Albrektsson T (2009) Classification of platelet concentrates: from pure platelet-rich plasma (P-PRP) to leucocyte-and platelet-rich fibrin (L-PRF). Trends Biotechnol 27:158–167

    Article  Google Scholar 

  23. Del Corso M, Vervelle A, Simonpieri A, Jimbo R, Inchingolo F, Sammartino G, Ehrenfest DMD (2012) Current knowledge and perspectives for the use of platelet-rich plasma (PRP) and platelet-rich fibrin (PRF) in oral and maxillofacial surgery part 1: periodontal and dentoalveolar surgery. Curr Pharm Biotechnol 13:1207–1230

    Article  Google Scholar 

  24. Simonpieri A, Del Corso M, Vervelle A, Jimbo R, Inchingolo F, Sammartino G, Ehrenfest DMD (2012) Current knowledge and perspectives for the use of platelet-rich plasma (PRP) and platelet-rich fibrin (PRF) in oral and maxillofacial surgery part 2: bone graft, implant and reconstructive surgery. Curr Pharm Biotechnol 13:1231–1256

    Article  Google Scholar 

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Acknowledgments

We would like to thank Mr. George Eckert for the statistical analyses.

Funding

The work was supported by a Young Researcher Grant provided by the Osteology Foundation, Switzerland (project number: 16-142) and the Indiana University School of Dentistry Graduate Student Research Committee.

Author information

Authors and Affiliations

  1. Department of Periodontics and Dental Hygiene, University of Texas Health Science Center at Houston School of Dentistry, 7500 Cambridge Street, Houston, TX, 77045, USA

    Jennifer Chang

  2. Department of Periodontics and Allied Dental Programs, Indiana University School of Dentistry, Indiana, USA

    Steven B. Blanchard & Yusuke Hamada

  3. Department of Biomedical and Applied Science, Indiana University School of Dentistry, 1121 W Michigan Street, Indianapolis, IN, 46202, USA

    L. Jack Windsor & Richard L. Gregory

Authors
  1. Jennifer Chang
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  2. Steven B. Blanchard
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  3. L. Jack Windsor
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  4. Richard L. Gregory
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  5. Yusuke Hamada
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Corresponding author

Correspondence to Yusuke Hamada.

Ethics declarations

All subjects were recruited from the Graduate Periodontics Clinic and the Comprehensive Care Clinics of Indiana University School of Dentistry with approval from the Indiana University Institutional Review Board (protocol# 1704165172) between September and November 2017.

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional research committee.

Informed consent

Informed consent was obtained from all individual participants included in the study.

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Chang, J., Blanchard, S.B., Windsor, L.J. et al. Levels of growth factors from platelet-rich fibrin from chronic periodontitis versus periodontally healthy subjects: a pilot study. Clin Oral Invest 24, 823–832 (2020). https://doi.org/10.1007/s00784-019-02944-7

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  • DOI: https://doi.org/10.1007/s00784-019-02944-7

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