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Gingival crevicular fluid and serum hCAP18/LL-37 levels in generalized aggressive periodontitis

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Abstract

Objectives

hCAP18/LL-37 is an endogenous antibiotic having a role in innate immunity. The aim of the present study was to evaluate serum and gingival crevicular fluid (GCF) hCAP18/LL-37 levels in patients with generalized aggressive periodontitis (G-AgP).

Materials and methods

Twenty-six G-AgP patients, 24 gingivitis patients, and 25 healthy subjects were included in this study. Periodontal parameters including probing depth, clinical attachment level, plaque index, and papilla bleeding index were recorded. GCF and serum hCAP18/LL-37 levels were analyzed by enzyme-linked immunosorbent assay.

Results

GCF hCAP18/LL-37 level was significantly higher in G-AgP compared to others (p = 0.038, p < 0.001). Gingivitis patients had significantly higher GCF hCAP18/LL-37 levels than controls (p < 0.001). No significant differences were observed in serum hCAP18/LL-37 levels among the study groups (p = 0.524). While there were positive correlations between GCF hCAP18/LL-37 levels and periodontal parameters of sampling sites (p < 0.005), no significant correlation was observed between serum hCAP18/LL-37 levels and whole-mouth periodontal parameters (p > 0.05).

Conclusion

Increased levels of GCF hCAP18/LL-37 in G-AgP might show that it is abundantly expressed in the presence of periodontal tissue destruction. Serum hCAP18/LL-37 levels do not seem to be related with the presence of G-AgP.

Clinical relevance

hCAP18/LL-37 antimicrobial peptide might be associated with periodontal tissue destruction in the presence of aggressive periodontitis.

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Authors and Affiliations

  1. Department of Periodontology, School of Dentistry, Ege University, Bornova, 35100, Izmir, Turkey

    Oya Turkoglu, Gulnur Emingil, Gülnihal Eren & Gül Atilla

  2. Department of Biology, School of Science and Letters, Celal Bayar University, Manisa, Turkey

    Harika Atmaca

  3. Department of Pediatrics, School of Medicine, Ege University, Izmir, Turkey

    Necil Kutukculer

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  1. Oya Turkoglu
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  2. Gulnur Emingil
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  5. Necil Kutukculer
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  6. Gül Atilla
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Correspondence to Gülnihal Eren.

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Funding

This study was supported by a grant from Ege University Research Foundation and from the authors themselves.

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All procedures performed in studies involving human participants were in accordance with the ethical standards of Ethics Committee of the Ege University Izmir, Turkey (#14–9.1/29), and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

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Informed consent was obtained from all individual participants included in the study.

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Turkoglu, O., Emingil, G., Eren, G. et al. Gingival crevicular fluid and serum hCAP18/LL-37 levels in generalized aggressive periodontitis. Clin Oral Invest 21, 763–769 (2017). https://doi.org/10.1007/s00784-016-1834-z

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  • DOI: https://doi.org/10.1007/s00784-016-1834-z

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