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Antibacterial and dissolution ability of sodium hypochlorite in different pHs on multi-species biofilms

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Abstract

Objectives

The aim of this study was to investigate whether variation in pH of sodium hypochlorite (NaOCl) increased its antibacterial and dissolution ability on polymicrobial biofilms formed in situ.

Materials and methods

Fifty-six dentin blocks (eight/group) were intraorally infected for 48 h and incubated in BHI for 48 h to standardize the biofilm growth. The specimens were irrigated with 1 and 2.5 % NaOCl with pH levels of 5, 7, and 12 for 20 min. The control group was irrigated with distilled water. The cell viability and the bacterial volume were measured at the pre- and post-irrigation procedures. Five random areas of each sample were chosen and analyzed with confocal laser scanning microscopy (CLSM). Statistical analysis was performed using the non-parametric Kruskal-Wallis and Dunn’s tests (p < 0.05).

Results

All the experimental solutions were able to decrease the biomass (p < 0.05) except for the 1 % NaOCl-pH 5 group. The antibacterial ability of the NaOCl was dependent on the concentration and acidification of the solution.

Conclusions

The acidification of NaOCl improves its antibacterial ability, but the dissolution effect of the irrigant is decreased.

Clinical relevance

Bacteria and their products are the main factors in development of apical periodontitis. The pH reduction in the NaOCl could enhance the reduction or elimination of the root canal bacterial colonies in comparison with the unaltered solution.

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Acknowledgments

This study was supported by the São Paulo Research Foundation (FAPESP 2011/08184-8) and the National Council for Scientific and Technological Development (CNPq 135419/2011-5).

Conflict of interest

The authors deny any conflicts of interest related to this study.

Author information

Authors and Affiliations

  1. Department of Dentistry, Endodontics and Dental Materials, Bauru Dental School, University of São Paulo, Faculdade de Odontologia de Bauru, USP. Al. Octavio Pinheiro Brisolla, 9-75, Bauru, SP, 17012-901, Brazil

    Aldo del Carpio-Perochena, Clovis Monteiro Bramante, Flaviana Bombarda de Andrade, Amanda G. Alves Maliza, Bruno Cavalini Cavenago, Marina A. Marciano, Pablo Amoroso-Silva & Marco Hungaro Duarte

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  1. Aldo del Carpio-Perochena
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  2. Clovis Monteiro Bramante
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  3. Flaviana Bombarda de Andrade
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  4. Amanda G. Alves Maliza
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  5. Bruno Cavalini Cavenago
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  6. Marina A. Marciano
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  7. Pablo Amoroso-Silva
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  8. Marco Hungaro Duarte
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Correspondence to Aldo del Carpio-Perochena.

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del Carpio-Perochena, A., Bramante, C.M., de Andrade, F.B. et al. Antibacterial and dissolution ability of sodium hypochlorite in different pHs on multi-species biofilms. Clin Oral Invest 19, 2067–2073 (2015). https://doi.org/10.1007/s00784-015-1431-6

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  • DOI: https://doi.org/10.1007/s00784-015-1431-6

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