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Effect of theobromine-containing toothpaste on dentin tubule occlusion in situ

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Abstract

Objectives

Dentin hypersensitivity (DH) is treated by either occlusion of dentin tubules or nerve desensitization. This in situ study compared dentin tubules occlusion by theobromine-containing dentifrices with (Theodent-classic-F®, TCF) and without (Theodent-classic®, TC) fluoride with 1,500 ppm fluoride toothpaste, Colgate®-Regular (Fluoride) and Novamin®-containing toothpaste, Sensodyne®-5000-Nupro (Novamin®).

Methods

Each subject wore four intraoral appliances bearing dentin blocks while using one of four test dentifrices (n = 20/dentifrice) twice daily for 7 days. The four appliances were removed successively after 1, 2, 3, and 7 days. Treated blocks and their control (untreated) blocks were examined with scanning electron microscopy (SEM). Effects were compared statistically (ANOVA/Tukey’s) based on percentage of surface area covered by deposited precipitate layer (%DPL) and percentage of fully open (%FOT), partially occluded (%POT), and completely occluded (%COT) tubules in each block calculated relative to the number of tubules in their control blocks.

Results

SEM observation indicated an increased %COT and %DPL over time. After 1 and 2 days, %COT was comparable with TC and TCF, and significantly (p < 0.05) higher compared with Novamin® and Fluoride. Following 3 and 7 days, %COT was comparable among TC, TCF, and Novamin®, but remained significantly lower in Fluoride. At any time, %DPL was significantly (p < 0.05) higher in TC, TCF, and Novamin® compared with Fluoride.

Conclusions

Theobromine-containing toothpastes with and without fluoride have equal potential in occluding dentin tubules within a shorter time period than Novamin®-containing toothpaste; however, the three demonstrated equal potential after 1 week, but not the fluoride toothpaste.

Clinical relevance

Theobromine-containing toothpaste promoted dentin tubule occlusion thus shows potential to relief DH.

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Acknowledgment

We are grateful to Theodent (New Orleans, LA) for their support in providing us with the Theodent toothpastes.

Conflict of interests

All authors declared no conflicts of interests, and no financial relationship with Theodent, (New Orleans, LA) that sponsored this research. Authors have full control of all primary data and agree to allow the journal to review their data if requested.

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Authors and Affiliations

  1. Department of Comprehensive Dentistry, University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, San Antonio, TX, 78229-3900, USA

    Bennett T. Amaechi, Sapna M. Mathews & Poornima K. Mensinkai

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  1. Bennett T. Amaechi
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  2. Sapna M. Mathews
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  3. Poornima K. Mensinkai
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Correspondence to Bennett T. Amaechi.

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Amaechi, B.T., Mathews, S.M. & Mensinkai, P.K. Effect of theobromine-containing toothpaste on dentin tubule occlusion in situ. Clin Oral Invest 19, 109–116 (2015). https://doi.org/10.1007/s00784-014-1226-1

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  • DOI: https://doi.org/10.1007/s00784-014-1226-1

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