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Biodegradable nanofibrous drug delivery systems: effects of metronidazole and ciprofloxacin on periodontopathogens and commensal oral bacteria

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Abstract

Objectives

The purposes of this study were to fabricate biodegradable polydioxanone (PDS II®) electrospun periodontal drug delivery systems (hereafter referred to as matrices) containing either metronidazole (MET) or ciprofloxacin (CIP) and to investigate the effects of antibiotic incorporation on both periodontopathogens and commensal oral bacteria.

Materials and methods

Fibrous matrices were processed from PDS polymer solution by electrospinning. Antibiotic-containing PDS solutions were prepared to obtain four distinct groups: 5 wt.% MET, 25 wt.% MET, 5 wt.% CIP, and 25 wt.% CIP. Pure PDS was used as a control. High-performance liquid chromatography (HPLC) was done to evaluate MET and CIP release. Dual-species biofilms formed by Lactobacillus casei (Lc) and Streptococcus salivarius (Ss) were grown on the surface of all electrospun matrices. After 4 days of biofilm growth, the viability of bacteria on biofilms was assessed. Additionally, antimicrobial properties were evaluated against periodontopathogens Fusobacterium nucleatum (Fn) and Aggregatibacter actinomycetemcomitans (Aa) using agar diffusion assay.

Results

A three-dimensional interconnected porous network was observed in the different fabricated matrices. Pure PDS showed the highest fiber diameter mean (1,158 ± 402 nm) followed in a descending order by groups 5 wt.% MET (1,108 ± 383 nm), 25 wt.% MET (944 ± 392 nm), 5 wt.% CIP (871 ± 309 nm), and 25 wt.% CIP (765 ± 288 nm). HPLC demonstrated that groups containing higher amounts (25 wt.%) of incorporated drugs released more over time, while those with lower levels (5 wt.%) the least. No inhibitory effect of the tested antibiotics was detected on biofilm formation by the tested commensal oral bacteria. Meanwhile, CIP-containing matrices inhibited growth of Fn and Aa.

Conclusion

CIP-containing matrices led to a significant inhibition of periodontopathogens without negatively impairing the growth of periodontal beneficial bacteria.

Clinical relevance

Based on the proven in vitro inhibition of periodontitis-related bacteria, future in vivo research using relevant animal models is needed to confirm the effectiveness of these drug delivery systems.

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Acknowledgments

This work was supported in part by the Research Support Funds Grant (RSFG) at Indiana University-Purdue University Indianapolis (IUPUI), by start-up funds from the IU School of Dentistry, and the NIH-NIDCR (Grant # DE023552) (all to M.C.B.).

Conflict of interest

The authors declare that they have no conflict of interest.

Author information

Authors and Affiliations

  1. Department of Restorative Dentistry, Division of Dental Biomaterials, Indiana University School of Dentistry (IUSD), 1121 W. Michigan Street, Indianapolis, IN, 46202, USA

    Marco C. Bottino & Krzysztof Kamocki

  2. Department of Preventive and Community Dentistry, IUSD, Indianapolis, IN, 46202, USA

    Rodrigo A. Arthur & R. Aaron Waeiss

  3. Department of Preventive and Community Dentistry, School of Dentistry, Federal University of Rio Grande do Sul, Porto Alegre, RS, 90035-003, Brazil

    Rodrigo A. Arthur

  4. Department of Oral Biology, IUSD, Indianapolis, IN, 46202, USA

    Karen S. Gregson & Richard L. Gregory

Authors
  1. Marco C. Bottino
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  2. Rodrigo A. Arthur
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  3. R. Aaron Waeiss
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  4. Krzysztof Kamocki
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  5. Karen S. Gregson
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Corresponding author

Correspondence to Marco C. Bottino.

Additional information

Marco C. Bottino and Rodrigo A. Arthur contributed equally to this manuscript

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Bottino, M.C., Arthur, R.A., Waeiss, R.A. et al. Biodegradable nanofibrous drug delivery systems: effects of metronidazole and ciprofloxacin on periodontopathogens and commensal oral bacteria. Clin Oral Invest 18, 2151–2158 (2014). https://doi.org/10.1007/s00784-014-1201-x

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  • DOI: https://doi.org/10.1007/s00784-014-1201-x

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