Abstract
Objectives
The aim of this study was to evaluate the physical properties and cytotoxicity of a novel root-end filling material (EPC) which is made from epoxy resin and Portland cement as a mineral trioxide aggregate (MTA) substitute.
Materials and methods
EPC, developed as a root-end filling material, was compared with MTA and a mixture of AH Plus sealer and MTA (AMTA) with regard to the setting time, radio-opacity, and microleakage. Setting times were evaluated using Vicat apparatus. Digital radiographs were taken to evaluate the aluminium equivalent radio-opacity using an aluminium step wedge. Extracted single-rooted teeth were used for leakage test using methylene blue dye. After canal shaping and obturation, the apical 3-mm root was resected, and a root-end cavity with a depth of 3 mm was prepared. The root-end cavities were filled with MTA, AMTA, and EPC for 15 specimens in each of three groups. After setting in humid conditions for 24 h, the specimens were tested for apical leakage. For evaluation of the biocompatibility of EPC, cell (human gingival fibroblast) viability was compared for MTA and Portland cement by MTT assay, and cell morphological changes were compared for MTA and AH Plus by fluorescence microscopy using DAPI and F-actin staining. The setting time, radio-opacity, and microleakage were compared using one-way ANOVA and Scheffe’s post hoc comparison, and the cytotoxicity was compared using the nonparametric Kruskal–Wallis rank sum test. Statistical significance was set at 95 %.
Results
EPC had a shorter setting time and less microleakage compared with MTA (p < 0.05). EPC showed 5-mm aluminium thickness radio-opacity and similar biocompatibility to MTA.
Conclusions
Under the conditions of this study, EPC, a novel composite made from a mixture of epoxy resin and Portland cement, was found to be a useful material for root-end filling, with favourable radio-opacity, short setting time, low microleakage, and clinically acceptable low cytotoxicity.
Clinical relevance
The novel root-end filling material would be a potentially useful material for a surgical endodontic procedure with favourable properties.
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References
Camilleri J, Pitt Ford TR (2006) Mineral trioxide aggregate: a review of the constituents and biological properties of the material. Int Endod J 39:747–754
Ford TR, Torabinejad M, Abedi HR, Bakland LK, Kariyawasam SP (1996) Using mineral trioxide aggregate as a pulp-capping material. J Am Dent Assoc 127:1491–1494
Salako N, Joseph B, Ritwik P, Salonen J, John P, Junaid TA (2003) Comparison of bioactive glass, mineral trioxide aggregate, ferric sulfate, and formocresol as pulpotomy agents in rat molar. Dent Traumatol 19:314–320
Bakland LK (2000) Management of traumatically injured pulps in immature teeth using MTA. J Calif Dent Assoc 28:855–858
Witherspoon DE, Ham K (2001) One-visit apexification: technique for inducing root-end barrier formation in apical closures. Pract Proced Aesthet Dent 13:455–460
Banchs F, Trope M (2004) Revascularization of immature permanent teeth with apical periodontitis: new treatment protocol? J Endod 30:196–200
Holden DT, Schwartz SA, Kirkpatrick TC, Schindler WG (2008) Clinical outcomes of artificial root-end barriers with mineral trioxide aggregate in teeth with immature apices. J Endod 34:812–817
Chng HK, Islam I, Yap AU, Tong YW, Koh ET (2005) Properties of a new root-end filling material. J Endod 31:665–668
Islam I, Chng HK, Yap AU (2006) Comparison of the physical and mechanical properties of MTA and portland cement. J Endod 32:193–197
Camilleri J (2008) The physical properties of accelerated Portland cement for endodontic use. Int Endod J 41:151–157
Torabinejad M, Hong CU, McDonald F, Pitt Ford TR (1995) Physical and chemical properties of a new root-filling material. J Endod 21:349–353
Sluyk SR, Moon PC, Hartwell GR (1998) Evaluation of setting properties and retention characteristics of mineral trioxide aggregate when used as a furcation perforation repair material. J Endod 24:768–771
Ber BS, Hatton JF, Stewart GP (2007) Chemical modification of ProRoot MTA to improve handling characteristics and decrease setting time. J Endod 33:1231–1234
Wiltbank KB, Schwartz SA, Schindler WG (2007) Effect of selected accelerants on the physical properties of mineral trioxide aggregate and portland cement. J Endod 33:1235–1238
Asgary S, Shahabi S, Jafarzadeh T, Amini S, Kheirieh S (2008) The properties of a new endodontic material. J Endod 34:990–993
Huang TH, Shie MY, Kao CT, Ding SJ (2008) The effect of setting accelerator on properties of mineral trioxide aggregate. J Endod 34:590–593
Saidon J, He J, Zhu Q, Safavi K, Spångberg LS (2003) Cell and tissue reactions to mineral trioxide aggregate and Portland cement. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 95:483–489
Ribeiro DA, Duarte MA, Matsumoto MA, Marques ME, Salvadori DM (2005) Biocompatibility in vitro tests of mineral trioxide aggregate and regular and white Portland cements. J Endod 31:605–607
Leyhausen G, Heil J, Reifferscheid G, Waldmann P, Geurtsen W (1999) Genotoxicity and cytotoxicity of the epoxy resin based root canal sealer AH plus. J Endod 25:109–113
Scarparo RK, Grecca FS, Fachin EV (2009) Analysis of tissue reactions to methacrylate resin-based, epoxy resin-based, and zinc oxide-eugenol endodontic sealers. J Endod 35:229–232
American Society for Testing and Materials (2004) Standard test method for time of setting of hydraulic cement by Vicat needle. ASTM C191-04. American Society for Testing and Materials, West Conshohocken
Kogan P, He J, Glickman GN, Watanabe I (2006) The effects of various additives on setting properties of MTA. J Endod 32:569–572
Hsieh SC, Teng NC, Lin YC et al (2009) A novel accelerator for improving the handling properties of dental filling materials. J Endod 35:1292–1295
Coomaraswamy KS, Lumley PJ, Hofmann MP (2007) Effect of bismuth oxide radioopacifier content on the material properties of an endodontic Portland cement-based (MTA-like) system. J Endod 33:295–298
Al-Hiyasat AS, Tayyar M, Darmani H (2010) Cytotoxicity evaluation of various resin based root canal sealers. Int Endod J 43:148–153
Oquntebi BR, Shen C (1992) Effect of different sealers on thermoplasticized Gutta-percha root canal obturations. J Endod 18:363–366
De Almeida WA, Leonardo MR, Tanomaru Filho M, Silva LA (2000) Evaluation of apical sealing of three endodontic sealers. Int Endod J 33:25–27
Kim JC, Kim MR, Ko HJ, Won KY (2009) Apical microleakage of MTA with 4-META/MMA & TBB resin as a root-end filling material. J Kor Acad Cons Dent 34:371–376
Wu MK, Kontakiotis EG, Wesselink PR (1998) Decoloration of 1 % methylene blue solution in contact with dental filling materials. J Dent 26:585–589
Fridland M, Rosado R (2003) Mineral trioxide aggregate (MTA) solubility and porosity with different water-to-powder ratios. J Endod 29:814–817
Dammaschke T, Gerth HU, Züchner H, Schäfer E (2005) Chemical and physical surface and bulk material characterization of white ProRoot MTA and two Portland cements. Dent Mater 21:731–738
International Organization for Standardization. ISO 6876–2001: Dental root sealing materials
Shah PMM, Chong BS, Sidhu SK, Ford TR (1996) Radiopacity of potential root-end filling materials. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 81:476–479
Saliba E, Abbassi-Ghadi S, Vowles R, Camilleri J, Hooper S, Camilleri J (2009) Evaluation of the strength and radiopacity of Portland cement with varying additions of bismuth oxide. Int Endod J 42:322–328
Camilleri J (2007) Hydration mechanism of mineral trioxide aggregate. Int Endod J 40:462–470
Mosmann T (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 65:55–63
Van Cruchten S, Van Den Broeck W (2002) Morphological and biochemical aspects of apoptosis, oncosis and necrosis. Anat Histol Embryol 31:214–223
Samara A, Sarri Y, Stravopodis D, Tzanetakis GN, Kontakiotis EG, Anastasiadou E (2011) A comparative study of the effects of three root-end filling materials on proliferation and adherence of human periodontal ment fibroblasts. J Endod 37:865–870
Acknowledgments
This work was supported by clinical research grant from Pusan National University Hospital (2011).
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The authors deny any conflicts of interest related to this study.
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Lee, SJ., Chung, J., Na, HS. et al. Characteristics of novel root-end filling material using epoxy resin and Portland cement. Clin Oral Invest 17, 1009–1015 (2013). https://doi.org/10.1007/s00784-012-0782-5
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DOI: https://doi.org/10.1007/s00784-012-0782-5