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In vitro investigation of fluorescence of carious dentin observed with a Soprolife® camera

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Abstract

Objectives

Our aim was to determine the origin of the red fluorescence of carious dentine observed with the Soprolife® camera.

Methods

We conducted in vitro studies to evaluate the origin of the red fluorescence using acids and matrix metalloproteinase (MMP) to mimic caries and methylglycoxal (MGO) to evaluate the effect of glycation reactions on the red fluorescence. In every step of these models, we detected the changes of dentin photonic response with Soprolife® in daylight mode and in treatment mode. A Raman spectroscopy analysis was performed to determine the variations of the dentin organic during the in vitro caries processes. Raman microscopy was performed to identify change in the collagen matrix of dentine.

Results

The red fluorescence observed in carious dentine using a Soprolife® camera corresponds to the brownish color observed using daylight. Demineralization using nitric acid induces a loss of the green fluorescence of dentine. The red fluorescence of carious dentine is resistant to acid treatment. Immersion of demineralized dentine in MGO induces a change of color from white to orange-red. This indicates that the Maillard reaction contributes to lesion coloration. Immersion of demineralized dentine in an MMP-1 solution followed by MGO treatment results in a similar red fluorescence. Raman microspectroscopy analysis reveals accumulation of AGE's product in red-colored dentine.

Conclusions

Our results provide important information on the origin of the fluorescence variation of dentine observed with the Soprolife® camera. We demonstrate that the red fluorescence of carious dentine is linked to the accumulation of Advanced Glycation End products (AGE).

Clinical relevance

The study provides a new biological basis for the red fluorescence of carious dentine and reinforces the importance of the Soprolife® camera in caries diagnostics.

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Acknowledgements

The authors would like to thank the support of the PHORMOST European network European Network of Excellence and David Bourgogne for the Raman microscopy (Institut Charles Gerhardt, Université Montpellier 2).

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. EA4203 Laboratory of Biology and Nano-science, Université Montpellier 1, Montpellier, France

    Ivan Panayotov, Hamideh Salehi, Hervé Tassery, Jacques Yachouh, Frédéric J. G. Cuisinier & Bernard Levallois

  2. Preventive and Restorative Department, Mediterranean University, Marseille, France

    Elodie Terrer & Hervé Tassery

Authors
  1. Ivan Panayotov
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  2. Elodie Terrer
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  3. Hamideh Salehi
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  4. Hervé Tassery
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  5. Jacques Yachouh
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  6. Frédéric J. G. Cuisinier
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  7. Bernard Levallois
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Corresponding author

Correspondence to Frédéric J. G. Cuisinier.

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Panayotov, I., Terrer, E., Salehi, H. et al. In vitro investigation of fluorescence of carious dentin observed with a Soprolife® camera. Clin Oral Invest 17, 757–763 (2013). https://doi.org/10.1007/s00784-012-0770-9

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  • DOI: https://doi.org/10.1007/s00784-012-0770-9

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