Abstract
Objectives
This study was carried out to histologically assess the effect of bone grafting materials extracted from bovine origin on the bone healing process either alone or when mixed with autologous platelet-rich plasma which could be used in many procedures of oral and maxillofacial bone and implant reconstructive surgery.
Materials and methods
Sixteen rabbits were used; three intrabony defects in the femur bone of each rabbit were created, one left unfilled for normal healing process and served as control, the second filled with xenogenic graft (Gen-Ox-lyophilized bovine bone organic matrix), and the third filled with(Gen-Ox-lyophilized bovine bone organic matrix) mixed with autologous platelet-rich plasma . Histological examination of the sections was performed after staining with H&E and Van Geison stains. The histomorphometric analysis including counting of bone cells (osteoblasts, osteocytes, and osteoclasts) with performance of osteon diameter and lamellar thickness at the end of the fourth week postoperatively was obtained.
Results
It has been shown that with the use of autologous platelet-rich plasma in combination with the xenogenic bone graft prepared from bovine origin, new bone formation and neovascularization were enhanced significantly when compared with xenogenic graft alone.
Conclusion
The addition of PRP to xenogenic bone substitute in small bone defects of the rabbit femur showed a histomorphometric increase in bone formation (at the fourth week of healing).
Clinical relevance
Platelet concentrate might be used to accelerate the osseointegration of enosseous dental implants.
Similar content being viewed by others
References
Maus U, Andereya S, Gravius S, Siebert GH, Ohnsorge JA, Niedhart C (2008) Lack of effect on bone healing of injectable BMP-2 augmented hyaluronic acid. Arch Orthop Trauma Surg 128:1461–1466
Lye KW, Deatherage JR, Waite PD (2008) The use of demineralized bone matrix for grafting during Lefort I and chin osteotomies: techniques and complications. J Oral Maxillofac Surg 66:1580–1585
Nacamuli RP, Longaker MT (2005) Bone induction in craniofacial defects. Orthod Craniofac Res 8:259–266
Eppley BL, Pietrzak WS, Blanton MW (2005) Allograft and alloplastic bone substitutes: a review of science and technology for the craniofacial surgeon. J Craniofac Surg 16:981–989
Bostrom MP, Seigerman DA (2005) The clinical use of allografts, demineralized bone matrices, synthetic bone graft substitutes and osteoinductive growth factors: a survey study. HSSJ 1:9–18
Torricelli P, Fini M, Giavaresi G, Rimondini L, Giardino R (2002) Characterization of bone defect repair in young and aged rat femur induced by xenogenic demineralized bone matrix. J Periodontol 73:1003–1009
Norton MR, Odell EW, Thompson ID, Cook RJ (2003) Efficacy of bovine bone mineral for alveolar study. Clin Oral Implants Res 14:775–783
Kim SG, Kim WK, Park JC, Kim HJ (2002) A comparative study of osseointegration of Avana implants in a demineralized freeze-dried bone alone or with platelet-rich plasma. J Oral Maxillofac Surg 60:1018–1025
Thaller SR, Hoyt J, Dart A, Borjeson K, Tesluk H (1994) Repair of experimental calvarial defects with Bio-oss particles and collagen sponges in a rabbit model. J Craniofacial Surg 5:242–246
Tözum TF, Demirlap B (2003) Platelet rich plasma. A promising innovation in dentistry. J Can Dent Assoc 69:664
Lynch SE, Genco RJ, Marx RE (1999) Tissue engineering, application in maxillofacial surgery and periodontics, 1st edn. Quintessence Pub Co., Chicago
Weibrich G, Kleis WK, Hafner G, Hitzler WE, Wagner W (2003) Comparison of platelet leukocyte, and growth factor levels in point-of-care platelet-enriched plasma prepared using a modified Curasan kit, with preparations received from a local blood bank. Clin Oral Implants Res 14:357–362
Galindo-Moreno P, Avila G, Fernandez-Barbero JE, Aguilar M, SanChez-Fernandez E, Cutando A, Wang HL (2007) Evaluation of sinus floor elevation using composite bone graft mixture. Clin Oral Implants Res 18:376–382
Marx RE, Carlson ER, EichstaedtRM SSR, Strauss JE, Georgeff KR (1998) Platelet-rich plasma growth factor enhancement for bone grafts. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 85:638–646
Simon EN, Merkx MAW, Shubi FM, Kalyanyama BM, Stoelinga PJW (2006) Reconstruction of the mandible after ablative surgery for the treatment of aggressive, benign odontogenic tumors in Tanzania: a preliminary study. Int J Oral Maxillofac Surg 35:421–426
Thorn JJ, Sørensen H, Weis-Fogh U, Andersen M (2004) Autologous fibrin glue with growth factors in reconstructive maxillofacial surgery. Int J Oral Maxillofac Surg 33:95–100
Fennis JPM, Stoelinga PJW, Jansen JA (2004) Mandibular reconstruction: a histological and histomorphometric study on the use of autogenous scaffolds, particulate cortico-cancellous bone grafts and platelet-rich plasma in goats. Int J Oral Maxillofac Surg 33:48–55
Kim SG, Chung CH, Kim YK, Park JC, Lim SC (2002) Use of particulate dentin-plaster of Paris combination with/without platelet-rich plasma in the treatment of bone defects around implants. Int J Oral Maxillofac Implants 17:86–94
Rocha FS, Ramos LMA, Batista JD, Barbosa DZ, Decbicibi P (2011) Organic bovine graft associated with PRP in rabbit calvaria. Int Arch Otorhinolaryngol 15:208–213
Schlegel KA, Donath K, Rupprecht S, Flak S, Zimmermann R, Felszeghy E, Wiiltfang J (2004) De novo bone formation using bovine collagen and platelet-rich plasma. Biomaterials 25:5387–5393
Schlegel KA, Kloss FR, Kessler P, Schultze-Mosgau S, Nkenke E, Wiltfang J (2003) Bone conditioning to enhance implant osseointegration: an experimental study in pigs. Int J Oral Maxillofac Implants 18:505–511
You TM, Choi BH, Li J, Jung JH, Lee HJ, Lee SH, Jeong SM (2007) The effect of platelet-rich plasma on bone healing around implants placed in bone defects treated with Bio-Oss: a pilot study in the dog tibia. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 103:e8–e12
Shayesteh YS, Khirsand A, Dehghan M, Ardestani MA (2005) Evaluation of platelet-rich plasma in combination with deproteinized bovine bone mineral in rabbit cranium. J Dent 2:127–134
Coles EF (1980) Veterinary clinical pathology, 3rd edn. Saunders, Philadelphia, pp 144–161
Guzzardella GA, Torricelli P, Nicoli-Aldini N, Giardino R (2003) Osseointegration of endosseous ceramic implant after post operative lower power laser stimulation: an in vivo comparative study. Clin Oral Implants Res 14:226–232
Qassim AH (2004) Histological and histochemical studies of human fetal liver during different stages of intrauterine life. M.Sc Thesis, College of Medicine, University of Mosul, Iraq
Marins LV, Cestari TM, Sottovia AD, Granjerio JM, Taga R (2004) Radiographic and histological study of perennial bone defect repair in rat calvaria after treatment with blocks of porous bovine organic graft material. J Appl Oral Sciences 12:62–69
Vajda EG, Kneissel M, Muggenburg B, Miller SC (1999) Increased intracortical bone remodeling during lactation in Beagle dogs. Biol Reprod 61:1439–1444
Munhoz EA, Bodanezi A, Cestari TM, Taga R, de Carvalho PSP, Junior OF (2011) Long-term rabbits bone response to titanium implants in the presence of inorganic bovine-derived graft. J Biomat Appl Feb 22 (in press)
Grageda E (2004) Platelet-rich plasma and bone graft materials: a review and a standerdized research protocol. Implant Dent 75:1668–1677
Nash T, Howlett C, Martin C, Steele J, Johnson KA, Hicklin DJ (1994) Effect of platelet-derived growth factor on tibial osteotomes in rabbit. Bone 15:203–208
Plachokova AS, van den Dolder J, Stoelinga PJ, Jansen JA (2007) Early effect of platelet-rich plasma on bone healing in combination with an osteoconductive material in rat cranial defects. Clin Oral Implants Res 18:244–251
Okuda K, Tai H, Tanabe K, Suzuki H, Sato T, Kawase T, Saito Y, Wolff LF, Yoshiex H (2005) Platelet-rich plasma combined with a porous hydroxyapatite graft for the treatment of infra bony periodontal defects in humans. A comparative controlled clinical study. Periodontol 76:890–898
Hanna R, Trejo P, Weltman R (2004) Treatment of intrabony defects with bovine-derived xenograft alone and in combination with platelet-rich plasma: a randomized clinical trial. J Periodontol 75:1668–1677
Furest G, Reinhard G, Tangl S, Sanroman F, Watzek G (2005) Enhanced bone to implant contact by platlet released growth factors in mandibular cortical bone. A histomorphometric study in minipig. Int J Oral maxillofac Implant 18:685–690
Raghoebar G, Schortinghuis J, Liem RS, Ruben JL, van der Wal JE, Vissink A (2005) Does platelet rich plasma promote remodelling of autologous bone graftes used for augmentation of the maxillary sinus floor? Clin Oral Implant Res 16:349–356
Sanchez A, Sheridan P, Eckert S, Al W (2005) Regenerative potential of platelet rich plasma added to xenogenic bone grafts in peri-implant defects: a histomorphometric analysis in dogs. J Periodontol 76:1637–1644
Beglundh T, Lindhe J (1997) Healing around implants placed in bone defects treated with Bio-Oss. An experimental study in the dog. Clin Oral Implants Res 8:117–124
Donos N, Bosshardt D, Lang N, Graziani F, Tonetti M, Karring T, Kostopoulos L (2005) Bone formation by enamel matrix proteins and xenografts: an experimanetal study in the rat ramus. Clin Oral Implants Res 16:140–146
Slotte C, Lundgren D (1999) Augmentation of calvarial tissue using non-permeable silicon domes and bovine bone mineral. An experimental study in the rat. Clin Oral Implants Res 10:468–476
Tsay RC, Vo J, Burke A, Eisig SB, Lu HH, Landesberg R (2005) Differential growth factor retention by platelet-rich plasma composites. J Oral Maxillofac Surg 63:521–528
Wiltfang J, Kloss FR, Kessler P, Nkenke E, Schultze-Mosgan S, Zimmermann R, Schlegel KA (2004) Effect of platelet rich plasma on bone healing in combination with autogenous bone and bone substitutes in critical size defect (an animal experiment). Clin Oral Implants Res 15:187–193
Kim ES, Park EJ, Choung PH (2001) Platelet concentrate and its effect on bone formation in calvarial defect: an experimental study in rabbits. J Prosthet Dent 86:428–433
Conflict of interest
The authors declare that they have no conflict of interest
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Kurikchy, M.Q., Al-Rawi, N.H., Ayoub, R.S. et al. Histological evaluation of bone healing using organic bovine bone in combination with platelet-rich plasma (an experimental study on rabbits). Clin Oral Invest 17, 897–904 (2013). https://doi.org/10.1007/s00784-012-0751-z
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00784-012-0751-z