Skip to main content

Advertisement

SpringerLink
Log in

Human and bovine pulp-derived cell reactions to dental resin cements

  • Original Article
  • Published:
Clinical Oral Investigations Aims and scope Submit manuscript

Abstract

Objectives

The objective of this study was to evaluate the cytotoxic reaction of transfected human pulp derived cells (tHPDC) and transfected bovine pulp derived cells (tBPDC) after exposure to resin cements [RelyX UnicemClicker (RX), MaxCem (MC), Panavia F 2.0 (PF), BisCem (BC), and Bistite II DC (BII)] and to compare it to the generation of reactive oxygen species (ROS).

Materials and methods

Set materials were extracted in culture medium, cell survival as a measure of cytotoxicity was determined photometrically using crystal violet after cells were exposed to the extracts for 24 h. The generation of ROS was detected by flow cytometry after cells were exposed to extract dilutions for 1 h.

Results

The ranking of the least to the most cytotoxic material was: RX < BII < PF < BC < MC for both cell lines, but for tHPDC, only MC and PF eluates were different from untreated controls. Generally, tBPDC were more susceptible to materials than tHPDC, but only for RX and BC was this difference statistically significant. All undiluted extracts increased ROS production in both cell lines but to a higher amount in tHPDC than in tBPDC.

Conclusions

tHPDC reacted less sensitive than tBPDC in the cytotoxicity test but with the same rank order of materials. In contrast, the cellular oxidative stress reaction was more pronounced in tHPDC than in tBPDC.

Clinical relevance

Depending on the residual dentine layer in deep cavities, biologically active resin monomers or additives released from resin cements may influence the dentine–pulp complex, for instance, its regenerative and reparative capacities.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  1. Burke FJ, Watts DC (1994) Fracture resistance of teeth restored with dentin-bonded crowns. Quintessence Int 25:335–340

    PubMed  Google Scholar 

  2. Hikita K, Van Meerbeek B, De Munck J, Ikeda T, Van Landuyt K, Maida T et al (2007) Bonding effectiveness of adhesive luting agents to enamel and dentin. Dent Mater 23:71–80

    Article  PubMed  Google Scholar 

  3. Abo-Hamar SE, Hiller KA, Jung H, Federlin M, Friedl KH, Schmalz G (2005) Bond strength of a new universal self-adhesive resin luting cement to dentin and enamel. Clin Oral Investig 9:161–167

    Article  PubMed  Google Scholar 

  4. Schmid-Schwap M, Franz A, König F, Bristela M, Lucas T, Piehslinger E et al (2009) Cytotoxicity of four categories of dental cements. Dent Mater 25:360–368

    Article  PubMed  Google Scholar 

  5. Bakopoulou A, Mourelatos D, Tsiftsoglou AS, Giassin NP, Mioglou E, Garefis P (2009) Genotoxic and cytotoxic effects of different types of dental cement on normal cultured human lymphocytes. Mutat Res 672:103–112

    Article  PubMed  Google Scholar 

  6. Kong N, Jiang T, Zhou Z, Fu J (2009) Cytotoxicity of polymerized resin cements on human dental pulp cells in vitro. Dent Mater 25:1371–1375

    Article  PubMed  Google Scholar 

  7. Thonemann B, Schmalz G (2000) Immortalization of bovine dental papilla cells with simian virus 40 large T antigen. Arch Oral Biol 45:857–869

    Article  PubMed  Google Scholar 

  8. Thonemann B, Schmalz G (2000) Bovine dental papilla-derived cells immortalized with HPV 18 E6/E7. Eur J Oral Sci 108:432–441

    Article  PubMed  Google Scholar 

  9. Galler KM, Schweikl H, Thonemann B, D'Souza RN, Schmalz G (2006) Human pulp-derived cells immortalized with Simian Virus 40 T-antigen. Eur J Oral Sci 114:138–146

    Article  PubMed  Google Scholar 

  10. Schweikl H, Spagnuolo G, Schmalz G (2006) Genetic and cellular toxicology of dental resin monomers. J Dent Res 85:870–877

    Article  PubMed  Google Scholar 

  11. Chang HH, Guo MK, Kasten FH, Chang MC, Huang GF, Wang YL et al (2005) Stimulation of glutathione depletion, ROS production and cell cycle arrest of dental pulp cells and gingival epithelial cells by HEMA. Biomaterials 26:745–753

    Article  PubMed  Google Scholar 

  12. Schweikl H, Hartmann A, Hiller KA, Spagnuolo G, Bolay C, Brockhoff G et al (2007) Inhibition of TEGDMA and HEMA-induced genotoxicity and cell cycle arrest by N-acetylcysteine. Dent Mater 23:688–695

    Article  PubMed  Google Scholar 

  13. Eckhardt A, Müller P, Hiller KA, Krifka S, Bolay C, Spagnuolo G et al (2010) Influence of TEGDMA on the mammalian cell cycle in comparison with chemotherapeutic agents. Dent Mater 26:232–241

    Article  PubMed  Google Scholar 

  14. Galler KM, Schweikl H, Hiller KA, Cavender AC, Bolay C, D'Souza RN, et al. (2010) TEGDMA Reduces Mineralization in Dental Pulp Cells. J Dent Res in press, doi: 10.1177/0022034510384618

  15. ISO 10993-12:2007. Biological evaluation of medical devices—part 12: sample preparation and reference materials. International Organization for Standardization, Case postale 56, CH-1211 Geneva 20, Switzerland

  16. Gilles RJ, Didier N, Denton M (1986) Determination of cell number in monolayer cultures. Anal Biochem 159:109–113

    Article  Google Scholar 

  17. Myhre O, Andersen JM, Aernes H, Fonnum F (2003) Evaluation of the probes 2′,7′-dichlorofluorescin diacetate, luminol, and lucigenin as indicators of reactive species formation. Biochem Pharmacol 65:1575–1582

    Article  PubMed  Google Scholar 

  18. Thonemann B, Schmalz G, Hiller KA, Schweikl H (2002) Responses of L929 mouse fibroblasts, primary and immortalized bovine dental papilla-derived cell lines to dental resin components. Dent Mater 18:318–323

    Article  PubMed  Google Scholar 

  19. McCarthy TJ, Hayes EP, Schwartz CS, Witz G (1994) The reactivity of selected acrylate esters toward glutathione and deoxyribonucleosides in vitro: structure-activity relationships. Fundam Appl Toxicol 22:543–548

    Article  PubMed  Google Scholar 

  20. Nocca G, D'Antò V, Desiderio C, Rossetti DV, Valletta R, Baquala AM et al (2010) N-acetyl cysteine directed detoxification of 2-hydroxyethyl methacrylate by adduct formation. Biomaterials 31:2508–2516

    Article  PubMed  Google Scholar 

  21. Walther UI, Siagian II, Walther SC, Reichl FX, Hickel R (2004) Antioxidative vitamins decrease cytotoxicity of HEMA and TEGDMA in cultured cell lines. Arch Oral Biol 49:125–131

    Article  PubMed  Google Scholar 

  22. Gerzina TM, Hume WR (1996) Diffusion of monomers from bonding resin-resin composite combinations through dentine in vitro. J Dent 24:125–128

    Article  PubMed  Google Scholar 

  23. Altintas SH, Usumez A (2008) Evaluation of monomer leaching from a dual cured resin cement. Biomed Mater Res B Appl Biomater 86B:523–529

    Article  Google Scholar 

  24. Ulker HE, Sengun A (2009) Cytotoxicity evaluation of self adhesive composite resin cements by dentin barrier test on 3D pulp cells. Eur J Dent 3:120–126

    PubMed  Google Scholar 

  25. Ratanasathien S, Wataha JC, Hanks CT, Dennison JB (1995) Cytotoxic interactive effects of dentin bonding components on mouse fibroblasts. J Dent Res 74:1602–1606

    Article  PubMed  Google Scholar 

  26. Geurtsen W, Lehmann F, Spahl W, Leyhausen G (1998) Cytotoxicity of 35 dental resin composite monomers/additives in permanent 3 T3 and three human primary fibroblast cultures. J Biomed Mater Res 41:474–480

    Article  PubMed  Google Scholar 

  27. de Mendonça AA, Souza PP, Costa CA (2007) Cytotoxic effects of hard-setting cements applied on the odontoblast cell line MDPC-23. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 104:102–108

    Article  Google Scholar 

  28. de Souza Costa CA, Hebling J, Randall RC (2006) Human pulp response to resin cements used to bond inlay restorations. Dent Mater 22:954–962

    Article  PubMed  Google Scholar 

  29. Camargo SE, Camargo CH, Hiller KA, Rode SM, Schweikl H, Schmalz G (2009) Cytotoxicity and genotoxicity of pulp capping materials in two cell lines. Int Endod J 42:227–237

    Article  PubMed  Google Scholar 

  30. Camargo CH, Camargo SE, Valera MC, Hiller KA, Schmalz G, Schweikl H (2009) The induction of cytotoxicity, oxidative stress, and genotoxicity by root canal sealers in mammalian cells. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 108:952–960

    Article  PubMed  Google Scholar 

Download references

Acknowledgements

This study was based on the work performed by H. Esra Ulker for the fulfillment of the degree of Doctor of Philosophy, Selcuk University, Turkey. This study was supported by the Department of Operative Dentistry and Periodontology, University of Regensburg, Germany; the Scientific and Technological Research Council of Turkey (TUBITAK); and Selcuk University BAP (06102038) and IADR (John Gray Fellowship—2007).

The authors gratefully acknowledge the skillful technical assistance of Carola Bolay (Department of Operative Dentistry and Periodontology, University of Regensburg) and special thanks to Dr. Mustafa Ulker (Department of Operative Dentistry, Selcuk University, Konya, Turkey) for his valuable contributions.

Conflict of interest

The authors do not have any conflict of interests.

Author information

Authors and Affiliations

  1. Konya Oral Health Hospital, Konya Agiz Dis Sagligi Merkezi, 42070, Selcuklu, Konya, Turkey

    Hayriye Esra Ülker

  2. Department of Operative Dentistry and Periodontology, University of Regensburg Medical Centre, Regensburg, Germany

    Karl Anton Hiller, Helmut Schweikl, Claudia Seidenader & Gottfried Schmalz

  3. Department of Operative Dentistry, Kırıkkale University, Kırıkkale, Turkey

    Abdulkadir Sengun

Authors
  1. Hayriye Esra Ülker
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Karl Anton Hiller
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Helmut Schweikl
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Claudia Seidenader
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Abdulkadir Sengun
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Gottfried Schmalz
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Hayriye Esra Ülker.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ülker, H.E., Hiller, K.A., Schweikl, H. et al. Human and bovine pulp-derived cell reactions to dental resin cements. Clin Oral Invest 16, 1571–1578 (2012). https://doi.org/10.1007/s00784-011-0657-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00784-011-0657-1

Keywords

Search

Navigation