Skip to main content

Advertisement

SpringerLink
Log in

The antibacterial effect of gas ozone after 2 months of in vitro evaluation

  • Original Article
  • Published:
Clinical Oral Investigations Aims and scope Submit manuscript

Abstract

The aim of this study was to evaluate the effect of HealOzone on two microorganisms, 4 and 8 weeks after treatment, using a tooth cavity model. Four groups of caries-free third molars (n = 12) were used (A, B, C and D). Three cavities were prepared into each tooth. After sterilization, groups A and B were inoculated with Streptococcus mutans, and groups C and D, with Lactobacillus casei for 48 h. One cavity of each tooth was used to evaluate the infection. After inoculation, groups B and D were treated with ozone (60 s), and groups A and C were used as controls. Then, the two cavities of each tooth were filled with composite, and the teeth were stored in sucrose medium. The restorations were removed after 4 and 8 weeks, respectively; dentin chips were collected, and the amount of microorganisms was determined. Ozone treatment reduced significantly the amount of S. mutans compared to the control group (p ≤ 0.05). This antibacterial effect was able to be seen after 4 (p = 0.0005) and 8 (p = 0.0002) weeks. No significant difference was found between the control and treated group as far as L. casei is concerned (p > 0.05). HealOzone (60 s) can provide some antibacterial treatment against S. mutans even after 8 weeks. However, an elimination of the microorganisms through HealOzone seems not to be possible. L. casei was more resistant to ozone. Although ozone exerts a significant antibacterial effect against S. mutans, it is probably not enough as the only antibacterial method, during the fillings therapy.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Anderson MH, Bales DJ, Omnell KA (1993) Modern management of dental caries: the cutting edge is not the dental bur. J Am Dent Assoc 124:36–44

    PubMed  Google Scholar 

  2. Brännström M (1986) The cause of postrestorative sensitivity and its prevention. J Endod 12:475–481

    Article  PubMed  Google Scholar 

  3. Feuerstein O, Matalon S, Slutzky H, Weiss EI (2007) Antibacterial properties of self-etching dental adhesive systems. J Am Dent Assoc 138:349–354

    PubMed  Google Scholar 

  4. Trowbridge HO (1981) Pathogenesis of pulpitis resulting from dental caries. J Endod 7:52–60

    Article  PubMed  Google Scholar 

  5. Imazato S, Imai T, Ebisu S (1998) Antibacterial activity of proprietary self-etching primers. Am J Dent 11:106–108

    PubMed  Google Scholar 

  6. Ozer F, Unlu N, Karakaya S, Ergani O, Hadimli HH (2005) Antibacterial activities of MDPB and fluoride in dentin bonding agents. Eur J Prosthodont Restor Dent 13:139–142

    PubMed  Google Scholar 

  7. Wicht MJ, Haak R, Schutt-Gerowitt H, Kneist S, Noack MJ (2004) Suppression of caries-related microorganisms in dentine lesions after short-term chlorhexidine or antibiotic treatment. Caries Res 38:436–441

    Article  PubMed  Google Scholar 

  8. Duque C, Negrini Tde C, Hebling J, Spolidorio DM (2005) Inhibitory activity of glass-ionomer cements on cariogenic bacteria. Oper Dent 30:636–640

    PubMed  Google Scholar 

  9. Takahashi Y, Imazato S, Kaneshiro AV, Ebisu S, Frencken JE, Tay FR (2006) Antibacterial effects and physical properties of glass-ionomer cements containing chlorhexidine for the ART approach. Dent Mater 22:647–652

    Article  PubMed  Google Scholar 

  10. van Dijken JW, Kalfas S, Litra V, Oliveby A (1997) Fluoride and mutans streptococci levels in plaque on aged restorations of resin-modified glass ionomer cement, compomer and resin composite. Caries Res 31:379–383

    Article  PubMed  Google Scholar 

  11. Bowden GH (1996) Mutans streptococci caries and chlorhexidine. J Can Dent Assoc 62:700, 703–707, review

    PubMed  Google Scholar 

  12. Baseren M, Yazici AR, Ozalp M, Dayangac B (2005) Antibacterial activity of different generation dentin-bonding systems. Quintessence Int 36:339–344

    PubMed  Google Scholar 

  13. Polydorou O, Pelz K, Hahn P (2006) Antibacterial effect of an ozone device and its comparison with two dentin-bonding systems. Eur J Oral Sci 114:349–353

    Article  PubMed  Google Scholar 

  14. Schmalz G, Ergücü Z, Hiller KA (2004) Effect of dentin on the antibacterial activity of dentin bonding agents. J Endod 30:352–358

    Article  PubMed  Google Scholar 

  15. Turkun LS, Ates M, Turkun M, Uzer E (2005) Antibacterial activity of two adhesive systems using various microbiological methods. J Adhes Dent 7:315–320

    PubMed  Google Scholar 

  16. Imazato S (2003) Antibacterial properties of resin composites and dentin bonding systems. Dent Mater 19:449–457

    Article  PubMed  Google Scholar 

  17. Millar B, Hodson N (2007) Assessment of the safety of two ozone delivery devices. J Dent 35:195–200

    Article  PubMed  Google Scholar 

  18. Nagayoshi M, Fukuizumi T, Kitamura C, Yano J, Terashita M, Nishihara T (2004) Efficacy of ozone on survival and permeability of oral microorganisms. Oral Microbiol Immunol 19:240–246

    Article  PubMed  Google Scholar 

  19. Sharma M, Hudson JB (2008) Ozone gas is an effective and practical antibacterial agent. Am J Infect Control 36:559–563

    Article  PubMed  Google Scholar 

  20. Baysan A, Lynch E (2005) The use of ozone in dentistry and medicine. Prim Dent Care 12:47–52

    Article  PubMed  Google Scholar 

  21. Baysan A, Lynch E (2006) The use of ozone in dentistry and medicine. Part 2. Ozone and root caries. Prim Dent Care 13:37–41

    Article  PubMed  Google Scholar 

  22. Baysan A, Whiley RA, Lynch E (2000) Antimicrobial effect of a novel ozone- generating device on micro-organisms associated with primary root carious lesions in vitro. Caries Res 34:498–501

    Article  PubMed  Google Scholar 

  23. Muller P, Guggenheim B, Schmidlin PR (2007) Efficacy of gasiform ozone and photodynamic therapy on a multispecies oral biofilm in vitro. Eur J Oral Sci 115:77–80

    Article  PubMed  Google Scholar 

  24. Nagayoshi M, Kitamura C, Fukuizumi T, Nishihara T, Terashita M (2004) Antimicrobial effect of ozonated water on bacteria invading dentinal tubules. J Endod 30:778–781

    Article  PubMed  Google Scholar 

  25. Baysan A, Beighton D (2004) Assessment of the ozone-mediated killing of bacteria in infected dentine associated with non-cavitated occlusal carious lesions. Caries Res 41:337–341

    Article  Google Scholar 

  26. Huth KC, Jakob FM, Saugel B, Cappello C, Paschos E, Hollweck R, Hickel R, Brand K (2006) Effect of ozone on oral cells compared with established antimicrobials. Eur J Oral Sci 114:435–440

    Article  PubMed  Google Scholar 

  27. Hems RS, Gulabivala K, Ng YL, Ready D, Spratt DA (2005) An in vitro evaluation of the ability of ozone to kill a strain of Enterococcus faecalis. Int Endod J 38:22–29

    Article  PubMed  Google Scholar 

  28. Baysan A, Lynch E (2004) Effect of ozone on the oral microbiota and clinical severity of primary root caries. Am J Dent 17:56–60

    PubMed  Google Scholar 

  29. Bocci V, Luzzi E, Corradeschi F, Paulesu L, Di Stefano A (1993) Studies on the biological effects of ozone: 3. An attempt to define conditions for optimal induction of cytokines. Lymphokine Cytokine Res 12:121–126

    PubMed  Google Scholar 

  30. Yamayoshi T, Tatsumi N (1993) Microbicidal effects of ozone solution on methicillin-resistant Staphylococcus aureus. Drugs Exp Clin Res 19:59–64

    PubMed  Google Scholar 

  31. Lezcano I, Pérez R, Sánchez E, Baluja Ch (1999) Ozone inactivation of Pseudomonas aeruginosa, Escherichia coli, Shigella sonneus and Salmonella typhimurium in water. Ozone Sci Eng 21:293–300

    Article  Google Scholar 

  32. Bezirtzoglou E, Cretoiu SM, Moldoveanu M, Alexopoulos A, Lazar V, Nakou M (2008) A quantitative approach to the effectiveness of ozone against microbiota organisms colonizing toothbrushes. J Dent 36:600–605

    Article  PubMed  Google Scholar 

  33. Onstad GD, Strauch S, Meriluoto J, Codd GA, Von Gunten U (2007) Selective oxidation of key functional groups in cyanotoxins during drinking water ozonation. Environ Sci Technol 41:4397–4404

    Article  PubMed  Google Scholar 

  34. Suarez S, Dodd MC, Omil F, von Gunten U (2007) Kinetics of triclosan oxidation by aqueous ozone and consequent loss of antibacterial activity: relevance to municipal wastewater ozonation. Water Res 41:2481–2490

    Article  PubMed  Google Scholar 

  35. Johansson E, Claesson R, van Dijken JW (2009) Antibacterial effect of ozone on cariogenic bacterial species. J Dent 37:449–453

    Article  PubMed  Google Scholar 

  36. Napimoga MH, de Oliveira R, Reis AF, Gonçalves RB, Giannini M (2007) In vitro antimicrobial activity of peroxide-based bleaching agents. Quintessence Int 38:528, e329-333 (online only)

    Google Scholar 

  37. Bradshaw DJ, Marsh PD (1998) Analysis of pH-driven disruption of oral microbial communities in vitro. Caries Res 32:456–462

    Article  PubMed  Google Scholar 

  38. Magni E, Ferrari M, Hickel R, Huth KC, Ilie N (2008) Effect of ozone gas application on the mechanical properties of dental adhesives bonded to dentin. Dent Mater 24:1428–1434

    Article  PubMed  Google Scholar 

  39. Zaura E, Buijs MJ, ten Cate JM (2007) Effects of ozone and sodium hypochlorite on caries-like lesions in dentin. Caries Res 41:489–492

    Article  PubMed  Google Scholar 

Download references

Conflict of interest

The authors declare that they have no conflict of interest.

Author information

Authors and Affiliations

  1. Department of Operative Dentistry and Periodontology, Dental School and Hospital, University Medical Center Freiburg, Freiburg, Germany

    Olga Polydorou, Ahmad Halili & Petra Hahn

  2. Department of Microbiology and Hygiene, Albert-Ludwigs-University Freiburg, Freiburg, Germany

    Anette Wittmer & Klaus Pelz

  3. Department of Operative Dentistry and Periodontology, Dental School and Hospital, Albert-Ludwigs University, Hugstetter Straße 55, 79106, Freiburg, Germany

    Olga Polydorou

Authors
  1. Olga Polydorou
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ahmad Halili
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Anette Wittmer
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Klaus Pelz
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Petra Hahn
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Olga Polydorou.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Polydorou, O., Halili, A., Wittmer, A. et al. The antibacterial effect of gas ozone after 2 months of in vitro evaluation. Clin Oral Invest 16, 545–550 (2012). https://doi.org/10.1007/s00784-011-0524-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00784-011-0524-0

Keywords

Search

Navigation