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Effects of aging on surface properties and adhesion of Streptococcus mutans on various fissure sealants

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Abstract

The aim of the present study was the quantification of Streptococcus mutans adhesion on ten widely used pit and fissure sealant materials and the correlation of these findings to surface roughness (R a) and surface free energy (SFE). Additionally, changes in streptococcal adhesion and surface parameters after water immersion and artificial aging have been investigated. Circular specimens of ten fissure sealants (seven resin-based composites, two glass ionomers, and one compomer) were made and polished. Surface roughness was determined by perthometer and SFE by goniometer measurements. Sealant materials were incubated with S. mutans suspension (2.5 h, 37°C), and adhering bacteria were quantified by using a biofluorescence assay in combination with an automated plate reader. Surface properties and S. mutans adhesion were measured prior to and after water immersion after 1 and 6 months and after additional thermocycling (5,000 cycles; 5°C/55°C). The tested sealants showed significant differences in S. mutans adhesion prior to and after the applied aging procedures. Aging resulted in slight increases (mostly <0.2 μm) in surface roughness, as well as in significant decreases in SFE and in significantly lower quantities of adhering bacteria. Ketac Bond and UltraSeal XT plus revealed the lowest adhesion potential after artificial aging. In general, the amount of adhering S. mutans was reduced after aging, which may be related to the decline in SFEs.

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Acknowledgements

The technical assistance of Gerlinde Held and Anja Dirscherl is appreciated. We are grateful to Monika Schoell for the linguistic revision of the manuscript.

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Prosthetic Dentistry, Regensburg University Medical Centre, 93042, Regensburg, Germany

    Ralf Bürgers, Tashiana Cariaga, Martin Rosentritt, Gerhard Handel & Sebastian Hahnel

  2. Institute of Physical and Theoretical Chemistry, University of Regensburg, 93042, Regensburg, Germany

    Rainer Müller

  3. Institute of Medical Microbiology and Hygiene, Regensburg University Medical Centre, 93042, Regensburg, Germany

    Udo Reischl

  4. Department of Prosthetic Dentistry, Regensburg University Medical Centre, Franz-Josef-Strauss-Allee 11, 93053, Regensburg, Germany

    Ralf Bürgers

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  1. Ralf Bürgers
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  2. Tashiana Cariaga
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  4. Martin Rosentritt
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  7. Sebastian Hahnel
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Correspondence to Ralf Bürgers.

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Bürgers, R., Cariaga, T., Müller, R. et al. Effects of aging on surface properties and adhesion of Streptococcus mutans on various fissure sealants. Clin Oral Invest 13, 419–426 (2009). https://doi.org/10.1007/s00784-009-0256-6

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  • DOI: https://doi.org/10.1007/s00784-009-0256-6

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