Skip to main content

Advertisement

SpringerLink
Log in

Candida albicans adhesion to composite resin materials

  • Original Article
  • Published:
Clinical Oral Investigations Aims and scope Submit manuscript

Abstract

The adhesion of Candida albicans to dental restorative materials in the human oral cavity may promote the occurrence of oral candidosis. This study aimed to compare the susceptibility of 14 commonly used composite resin materials (two compomers, one ormocer, one novel silorane, and ten conventional hybrid composites) to adhere Candida albicans. Differences in the amount of adhering fungi should be related to surface roughness, hydrophobicity, and the type of matrix. Cylindrical specimens of each material were made according to the manufacturers’ instructions. Surface roughness R a was assessed by perthometer measurements and the degree of hydrophobicity by computerized contact angle analysis. Specimens were incubated with a reference strain of C. albicans (DMSZ 1386), and adhering fungi were quantified by using a bioluminometric assay in combination with an automated plate reader. Statistical differences were analyzed by the Kruskal–Wallis test and Mann–Whitney U test. Spearman’s rank correlation coefficients were calculated to assess correlations. Median R a of the tested composite resin materials ranged between 0.04 and 0.23 μm, median contact angles between 69.2° and 86.9°. The two compomers and the ormocer showed lower luminescence intensities indicating less adhesion of fungi than all tested conventional hybrid composites. No conclusive correlation was found between surface roughness, hydrophobicity, and the amount of adhering C. albicans.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  1. Arendorf TM, Walker DM (1987) Denture stomatitis: a review. J Oral Rehabil 14:217–227

    Article  PubMed  Google Scholar 

  2. Bollen CM, Lambrechts P, Quirynen M (1997) Comparison of surface roughness of oral hard materials to the threshold surface roughness for bacterial plaque retention: a review of the literature. Dent Mater 13:258–269

    Article  PubMed  Google Scholar 

  3. Blankenship JR, Mitchell AP (2006) How to build a biofilm: a fungal perspective. Curr Opin Microbiol 9:588–594

    Article  PubMed  Google Scholar 

  4. Budtz-Jorgensen E (1974) The significance of Candida albicans in denture stomatitis. Scand J Dent Res 82:151–190

    PubMed  Google Scholar 

  5. Budtz-Jorgensen E, Stenderup A, Grabowski M (1975) An epidemiologic study of yeasts in elderly denture wearers. Community Dent Oral Epidemiol 3:115–119

    Article  PubMed  Google Scholar 

  6. Buergers R, Rosentritt M, Handel G (2007) Bacterial adhesion of Streptococcus mutans to provisional fixed prosthodontic material. J Prosthet Dent 98:461–469

    Article  PubMed  Google Scholar 

  7. Cannon RD, Chaffin WL (1999) Oral colonization by Candida albicans. Crit Rev Oral Biol Med 10:359–383

    Article  PubMed  Google Scholar 

  8. Crouch SP, Kozlowski R, Slater KJ, Fletcher J (1993) The use of ATP bioluminescence as a measure of cell proliferation and cytotoxicity. J Immunol Methods 160:81–88

    Article  PubMed  Google Scholar 

  9. Davies AN, Brailsford SR, Beighton D, Shorthose K, Stevens VC (2008) Oral candidosis in community-based patients with advanced cancer. J Pain Symptom Manage 35:508–514

    Article  PubMed  Google Scholar 

  10. Dexter SJ, Camara M, Davies M, Shakesheff KM (2003) Development of a bioluminescent ATP assay to quantify mammalian and bacterial cell numbers from a mixed population. Biomaterials 24:27–34

    Article  PubMed  Google Scholar 

  11. Edgerton M, Scannapieco FA, Reddy MS, Levine MJ (1993) Human submandibular-sublingual saliva promotes adhesion of Candida albicans to polymethylmethacrylate. Inf Immun 61:2644–2652

    Google Scholar 

  12. Edgerton M, Raj PA, Levine MJ (1995) Surface-modified poly(methyl methacrylate) enhances adsorption and retains anticandidal activities of salivary histatin 5. J Biomed Mater Res 29:1277–1286

    Article  PubMed  Google Scholar 

  13. Elguezabal N, Maza JL, Ponton J (2004) Inhibition of adherence of Candida albicans and Candida dubliniensis to a resin composite restorative dental material by salivary secretory IgA and monoclonal antibodies. Oral Dis 10:81–86

    Article  PubMed  Google Scholar 

  14. Guggenberger R, Weinmann W (2000) Exploring beyond methacrylates. Am Dent J 13:82D–84D

    Google Scholar 

  15. Hirota K, Murakami K, Nemoto K, Miyake Y (2005) Coating of a surface with 2-methacryloyloxyethyl phosphorylcholine (MPC) co-polymer significantly reduces retention of human pathogenic microorganisms. FEMS Microbiol Lett 248:37–45

    Article  PubMed  Google Scholar 

  16. Kawai K, Takaoka T (2001) Inhibition of bacterial and glucan adherence to various light-cured fluoride-releasing restorative materials. J Dent 29:119–122

    Article  PubMed  Google Scholar 

  17. Klein RS, Harris CA, Small CB, Moll B, Lesser M, Friedland GH (1984) Oral candidiasis in high-risk patients as the initial manifestation of the acquired immunodeficiency syndrome. N Engl J Med 311:354–358

    PubMed  Google Scholar 

  18. Klotz SA, Drutz DJ, Zajic JE (1985) Factors governing adherence of Candida species to plastic surfaces. Infect Immun 50:97–101

    PubMed  Google Scholar 

  19. Klotz SA (1994) The contribution of electrostatic forces to the process of adherence of Candida albicans yeast cells to substrates. FEMS Microbiol Lett 120:257–262

    Article  PubMed  Google Scholar 

  20. Leibovici V, Alkalay R, Hershko K, Ingber A, Westerman M, Leviatan-Strauss N (2008) Prevalence of Candida on the tongue and intertriginous areas of psoriatic and atopic dermatitis patients. Mycoses 51:63–66

    PubMed  Google Scholar 

  21. Manhart J, Kunzelmann KH, Chen HY, Hickel R (2000) Mechanical properties of new composite restorative materials. J Biomed Mater Res 53:353–361

    Article  PubMed  Google Scholar 

  22. Maza JL, Elguezabal N, Prado C, Ellacuria J, Soler I, Ponton J (2002) Candida albicans adherence to resin-composite restorative dental material: influence of whole human saliva. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 94:589–592

    Article  PubMed  Google Scholar 

  23. Meyer JM, Cattani-Lorente MA, Dupuis V (1998) Compomers: between glass-ionomer cements and composites. Biomat 19:529–539

    Article  Google Scholar 

  24. Minagi S, Miyake Y, Inagaki K, Tsuru H, Suginaka H (1985) Hydrophobic interaction in Candida albicans and Candida tropicalis adherence to various denture base resin materials. Infect Immun 47:11–14

    PubMed  Google Scholar 

  25. Miyake Y, Fujita Y, Minagi S, Suginaka H (1986) Surface hydrophobicity and adherence of Candida to acrylic surfaces. Microbios 46:7–14

    PubMed  Google Scholar 

  26. Nevzatoglu EU, Özcan M, Kulak-Ozkan Y, Kadir T (2007) Adherence of Candida albicans to denture base acrylics and silicone-based resilient liner materials with different surface finishes. Clin Oral Investig 11:231–236

    Article  PubMed  Google Scholar 

  27. Nikawa H, Iwanaga H, Kameda M, Hamada T (1992) In vitro evaluation of Candida albicans adherence to soft denture-lining materials. J Prosthet Dent 68:804–808

    Article  PubMed  Google Scholar 

  28. Nikawa H, Yamamoto T, Hayashi S, Nikawa Y, Hamada T (1994) Growth and/or acid production of Candida albicans on soft lining materials in vitro. J Oral Rehabil 21:585–594

    Article  PubMed  Google Scholar 

  29. Nokta M (2008) Oral manifestations associated with HIV infection. Curr HIV/AIDS Rep 5:5–12

    Article  PubMed  Google Scholar 

  30. Okita N, Orstavik D, Orstavik J, Ostby K (1991) In vivo and in vitro studies on soft denture materials: microbial adhesion and tests for antibacterial activity. Dent Mater 7:155–160

    Article  PubMed  Google Scholar 

  31. Park SE, Periathamby AR, Loza JC (2003) Effect of surface-charged poly(methyl methacrylate) on the adhesion of Candida albicans. J Prosthodont 12:249–254

    PubMed  Google Scholar 

  32. Pereira T, Cury AA, Cenci MS, Rodrigues-Garcia RC (2007) In vitro Candida colonization on acrylic resins and denture liners: influence of surface free energy, roughness, saliva, and adhering bacteria. Int J Prosthodont 20:308–310

    PubMed  Google Scholar 

  33. Radford DR, Sweet SP, Challacombe SJ, Walter JD (1998) Adherence of Candida albicans to denture-base materials with different surface finishes. J Dent 26:577–583

    Article  PubMed  Google Scholar 

  34. Radford DR, Challacombe SJ, Walter JD (1999) Denture plaque and adherence of Candida albicans to denture-base materials in vivo and in vitro. Crit Rev Oral Biol Med 10:99–116

    Article  PubMed  Google Scholar 

  35. Rawls HR, Zimmerman BF (1983) Fluoride-exchanging resins for caries protection. Caries Res 17:32–43

    Article  PubMed  Google Scholar 

  36. Samaranayake LP, McCourtie J, MacFarlane TW (1980) Factors affecting the in-vitro adherence of Candida albicans to acrylic surfaces. Arch Oral Biol 25:611–615

    Article  PubMed  Google Scholar 

  37. Schweikl H, Schmalz G, Weinmann W (2002) Mutagenic activity of structurally related oxiranes and siloranes in Salmonella typhimurium. Mutat Res 521:19–27

    PubMed  Google Scholar 

  38. Segal E, Lehrman O, Dayan D (1988) Adherence in vitro of various Candida species to acrylic surfaces. Oral Surg Oral Med Oral Pathol 66:670–673

    Article  PubMed  Google Scholar 

  39. Serrano-Granger C, Cerero-Lapiedra R, Campo-Trapero J, Del Rio-Highsmith J (2005) In vitro study of the adherence of Candida albicans to acrylic resins: relationship to surface energy. Int J Prosthodont 18:392–398

    PubMed  Google Scholar 

  40. Ship JA (2003) Diabetes and oral health: an overview. J Am Dent Assoc 134:4S–10S

    PubMed  Google Scholar 

  41. Tronchin G, Bouchara JP, Robert R, Senet JM (1988) Adherence of Candida albicans germ tubes to plastic: ultrastructural and molecular studies of fibrilar adhesions. Infect Immun 56:1987–1993

    PubMed  Google Scholar 

  42. Tsang CS, Ng H, McMillan AS (2007) Antifungal susceptibility of Candida albicans biofilms on titanium discs with different surface roughness. Clin Oral Investig 11:361–368

    Article  PubMed  Google Scholar 

  43. Vandenbussche M, Swinne D (1984) Yeasts oral carriage in denture wearers. Mykosen 27:431–435

    PubMed  Google Scholar 

  44. Waltimo T, Tanner J, Vallittu P, Haapasalo M (1999) Adherence of Candida albicans to the surface of polymethylmethacrylate-E glass fiber composite used in dentures. Int J Prosthodont 12:83–86

    PubMed  Google Scholar 

  45. Waters MG, Williams DW, Jagger RG, Lewis MA (1997) Adherence of Candida albicans to experimental denture soft lining materials. J Prosthet Dent 77:306–312

    Article  PubMed  Google Scholar 

  46. Weinmann W, Thalacker C, Guggenberger R (2005) Siloranes in dental composites. Dent Mater 21:68–74

    Article  PubMed  Google Scholar 

  47. Yamauchi M, Yamamoto K, Wakabayashi M, Kawano J (1990) In vitro adherence of microorganisms to denture base resin with different surface texture. Dent Mater J 9:19–24

    PubMed  Google Scholar 

Download references

Acknowledgments

We gratefully acknowledge the technical assistance of Gerlinde Held. We are grateful to Justine Rochon for the statistical revision of the manuscript.

Conflict of interests

We did not receive financial or material support from any institution. We declare that there is no conflict of interests.

Author information

Authors and Affiliations

  1. Department of Prosthetic Dentistry, Regensburg University Medical Centre, Franz-Josef-Strauß-Allee, 93042, Regensburg, Germany

    Ralf Bürgers, Martin Rosentritt, Gerhard Handel & Sebastian Hahnel

  2. Institute of Medical Microbiology and Hygiene, Regensburg University Medical Centre, Franz-Josef-Strauß-Allee, 93042, Regensburg, Germany

    Wulf Schneider-Brachert

Authors
  1. Ralf Bürgers
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Wulf Schneider-Brachert
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Martin Rosentritt
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Gerhard Handel
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Sebastian Hahnel
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Ralf Bürgers.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Bürgers, R., Schneider-Brachert, W., Rosentritt, M. et al. Candida albicans adhesion to composite resin materials. Clin Oral Invest 13, 293–299 (2009). https://doi.org/10.1007/s00784-008-0226-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00784-008-0226-4

Keywords

Search

Navigation