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hBMP-2 and hTGF-β1 expressed in implanted BMSCs synergistically promote the repairing of segmental bone defects

  • Original Article
  • Published:
Journal of Orthopaedic Science

Abstract

Objective

To evaluate the effects of co-expressing hBMP-2 and hTGF-β1 in BMSCs (bone marrow-derived mesenchymal stem cells) on the repairing process of radial segmental defects in rats.

Methods

BMSCs were infected with a high titer recombinant adenovirus carrying hTGF-βl and/or hBMP-2 genes. Expression of exogenous genes in BMSCs was confirmed by RT-PCR and ELISA assays. In vitro effects of exogenous genes were assessed by MTT and ALP activity tests. A left radial defect model was created using 120 SD rats. Genetically modified or unmodified BMSCs were implanted with collagen sponge scaffolds into the 5-mm radial defect. The bone repair process was systematically monitored and evaluated by X-ray examinations, gross anatomic examinations, histological analyses, and biomechanical tests.

Results

Expression of hBMP-2 and hTGF-β1 showed synergistic effects on promoting BMSC proliferation and enhancing ALP activity in vitro. Bone repair assays showed that hBMP-2 and hTGF-β1 promoted the production of chondrocytes and osteoblasts. Implanted BMSCs transfected with both hBMP-2 and hTGF-β1 led to the best bone repair outcome.

Conclusion

hBMP-2 and hTGF-β1 can synergistically improve the bone repair process. Our results suggest a potential clinical value of combining hBMP-2 and hTGF-β1 in repairing bone defects.

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Acknowledgments

This research was supported by the China Science and Technology Innovation Program for Youth.

Conflict of interest

All authors of this article certified that they have no conflict of interest to declare.

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Authors and Affiliations

  1. Department of Orthopedics, Fuzhou General Hospital, 156 Xi Er Huan Road, Fuzhou, 350025, Fujian Province, Peopleʼs Republic of China

    Chenghui Yin, Jianping Chen, Zongxiong Chen, Zhaoxun Zeng & Junqin Qiu

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  1. Chenghui Yin
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  2. Jianping Chen
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  3. Zongxiong Chen
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  4. Zhaoxun Zeng
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  5. Junqin Qiu
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Correspondence to Chenghui Yin.

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Yin, C., Chen, J., Chen, Z. et al. hBMP-2 and hTGF-β1 expressed in implanted BMSCs synergistically promote the repairing of segmental bone defects. J Orthop Sci 20, 717–727 (2015). https://doi.org/10.1007/s00776-015-0714-8

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  • DOI: https://doi.org/10.1007/s00776-015-0714-8

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