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Immunohistological localization of Notch receptors and their ligands Delta and Jagged in synovial tissues of rheumatoid arthritis

  • Original article
  • Published:
Journal of Orthopaedic Science

Abstract

Background

The interaction of Notch receptors with their transmembrane ligands Delta and Jagged plays an important role not only in the organization of a variety of tissues but also in several genetic disorders and cancer development. The functional involvement of the Notch signaling in rheumatoid arthritis (RA) has been reported previously, but the expression profile of Notch-related molecules, as well as their relation with clinicopathological parameters, remains unclear.

Methods

In this study, we analyzed the immunohistochemical staining pattern of four Notch receptors (Notch1–4) and their ligands (Delta1 and Jagged1) in 14 synovial tissues obtained from 14 RA patients.

Results

Notch2 and Notch4 were expressed in limited areas in a few samples or in small blood vessels, respectively. Notch1, Notch3, Delta1, and Jagged1 were overexpressed in the synovial lining and sublining cells on synovial hyperplastic lesions in all samples. Notch1 expression was also observed in T and B lymphocytes of lymphoid follicles independently. Notch1 and Notch3 expression overlapped with that of Jagged1, as determined by confocal microscopy. Activation of Notch1 signaling in the RA synovium was identified using a specific antibody to the cleaved form of Notch1. The expression of these molecules did not show any correlation with clinicopathological parameters.

Conclusions

Our results suggest that Notch signaling is activated in RA synovium but does not necessarily reflect the pathological condition of RA.

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Authors and Affiliations

  1. Division of Orthopaedic Pathomechanism, Department of Developmental and Reconstructive Medicine, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan

    Tomoko Matsumoto, Toshiyuki Tsurumoto & Hiroyuki Shindo

  2. Department of Orthopaedic Surgery, Mitsubishi Nagasaki Hospital, 1-37 Koinoura, Nagasaki, 852-8588, Japan

    Yoshihiro Yabe

Authors
  1. Yoshihiro Yabe
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  2. Tomoko Matsumoto
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  3. Toshiyuki Tsurumoto
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  4. Hiroyuki Shindo
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Yabe, Y., Matsumoto, T., Tsurumoto, T. et al. Immunohistological localization of Notch receptors and their ligands Delta and Jagged in synovial tissues of rheumatoid arthritis. J Orthop Sci 10, 589–594 (2005). https://doi.org/10.1007/s00776-005-0943-3

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  • DOI: https://doi.org/10.1007/s00776-005-0943-3

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