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Structure of the UreD–UreF–UreG–UreE complex in Helicobacter pylori: a model study

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Abstract

The molecular details of the protein complex formed by UreD, UreF, UreG, and UreE, accessory proteins for urease activation in the carcinogenic bacterium Helicobacter pylori, have been elucidated using computational modeling. The calculated structure of the complex supports the hypothesis of UreF acting as a GTPase activation protein that facilitates GTP hydrolysis by UreG during urease maturation, and provides a rationale for the design of new drugs against infections by ureolytic bacterial pathogens.

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Acknowledgments

The project was supported by grants from the Italian MIUR and by the University of Bologna to S.C.; F.M. was supported by Programma Operativo del Fondo Sociale Europeo 2007/2013 of Regione Autonoma Friuli Venezia Giulia and by Consorzio Interuniversitario di Risonanze Magnetiche di Metallo Proteine (CIRMMP).

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Author notes

  1. Francesco Musiani

    Present address: International School for Advanced Studies (SISSA/ISAS), Trieste, Italy

Authors and Affiliations

  1. Laboratory of Bioinorganic Chemistry, Department of Pharmacy and Biotechnology, University of Bologna, Bologna, Italy

    Francesco Biagi, Francesco Musiani & Stefano Ciurli

Authors
  1. Francesco Biagi
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  2. Francesco Musiani
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  3. Stefano Ciurli
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Correspondence to Francesco Musiani or Stefano Ciurli.

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Results of the analysis conducted on the surface of HpUreF and UreG (PDF 1048 kb)

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Biagi, F., Musiani, F. & Ciurli, S. Structure of the UreD–UreF–UreG–UreE complex in Helicobacter pylori: a model study. J Biol Inorg Chem 18, 571–577 (2013). https://doi.org/10.1007/s00775-013-1002-8

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  • DOI: https://doi.org/10.1007/s00775-013-1002-8

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