Abstract
Members of the ZIP (SLC39A) and ZnT (SLC30A) families of transmembrane domain proteins are predicted to transport the essential transition metal zinc across membranes, regulating cellular zinc content and distribution via uptake and efflux at the outer plasma and organellar membranes. Twenty-four ZIP and ZnT proteins are encoded in mammalian genomes, raising questions of whether all actually transport zinc, whether several function together in the same tissues/cell types, and how the activity of these transporters is coordinated. To address these questions, we have taken advantage of the ability to manipulate several genes simultaneously in targeted cell types in Drosophila. Previously we reported zinc toxicity phenotypes caused by combining overexpression of a zinc uptake gene, dZip42C.1, with suppression of a zinc efflux gene, dZnT63C. Here we show that these phenotypes can be used as a sensitized in vivo system to detect subtle alterations in zinc transport activity that would be buffered in healthy cells. Using two adult tissues, the fly eye and midline (thorax/abdomen), we find that when overexpressed, most of the 17 Drosophila Zip and ZnT genes modify the zinc toxicity phenotypes in a manner consistent with their predicted zinc transport activity. In most cases, we can reconcile that activity with the cellular localization of an enhanced green fluorescent protein tagged version of the protein. Additionally, targeted suppression of each gene by RNA interference reveals several of the fly Zip and ZnT genes are required in the eye, indicating that numerous independent zinc transport genes are acting together in a single tissue.
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References
Berg JM, Shi Y (1996) Science 271:1081–1085
Tubek S, Grzanka P, Tubek I (2008) Biol Trace Elem Res 122:193–196
Kambe T, Weaver BP, Andrews GK (2008) Genesis 46:214–228
Dufner-Beattie J, Wang F, Kuo YM, Gitschier J, Eide D, Andrews GK (2003) J Biol Chem 278:33474–33481
Fukada T, Civic N, Furuichi T, Shimoda S, Mishima K, Higashiyama H, Idaira Y, Asada Y, Kitamura H, Yamasaki S, Hojyo S, Nakayama M, Ohara O, Koseki H, Dos Santos HG, Bonafe L, Ha-Vinh R, Zankl A, Unger S, Kraenzlin ME, Beckmann JS, Saito I, Rivolta C, Ikegawa S, Superti-Furga A, Hirano T (2008) PLoS ONE 3:e3642
Gupta A, Lutsenko S (2009) Future Med Chem 1:1125–1142. doi:10.4155/fmc.09.84
Fukada T, Yamasaki S, Nishida K, Murakami M, Hirano T (2011) J Biol Inorg Chem 16:1123–1134. doi:10.1007/s00775-011-0797-4
Andreini C, Banci L, Bertini I, Rosato A (2006) J Proteome Res 5:3173–3178
McMahon RJ, Cousins RJ (1998) Proc Natl Acad Sci USA 95:4841–4846
Nitzan YB, Sekler I, Hershfinkel M, Moran A, Silverman WF (2002) Brain Res Dev Brain Res 137:149–157
Sekler I, Moran A, Hershfinkel M, Dori A, Margulis A, Birenzweig N, Nitzan Y, Silverman WF (2002) J Comp Neurol 447:201–209. doi:10.1002/cne.10224
Elgazar V, Razanov V, Stoltenberg M, Hershfinkel M, Huleihel M, Nitzan YB, Lunenfeld E, Sekler I, Silverman WF (2005) J Histochem Cytochem 53:905–912. doi:10.1369/jhc.4A6482.2005
Palmiter RD, Cole TB, Findley SD (1996) EMBO J 15:1784–1791
Seo YA, Lopez V, Kelleher SL (2011) Am J Physiol Cell Physiol 300:C1479–C1489. doi:10.1152/ajpcell.00420.2010
Lopez V, Kelleher SL (2009) Biochem J 422:43–52. doi:10.1042/BJ20081189
Guo L, Lichten LA, Ryu MS, Liuzzi JP, Wang F, Cousins RJ (2010) Proc Natl Acad Sci USA 107:2818–2823. doi:10.1073/pnas.0914941107
Palmiter RD, Cole TB, Quaife CJ, Findley SD (1996) Proc Natl Acad Sci USA 93:14934–14939
Murgia C, Vespignani I, Cerase J, Nobili F, Perozzi G (1999) Am J Physiol 277:G1231–G1239
Ho LH, Ruffin RE, Murgia C, Li L, Krilis SA, Zalewski PD (2004) J Immunol 172:7750–7760
Murgia C, Grosser D, Truong-Tran AQ, Roscioli E, Michalczyk A, Ackland ML, Stoltenberg M, Danscher G, Lang C, Knight D, Perozzi G, Ruffin RE, Zalewski P (2011) Nutrients 3:910–928. doi:10.3390/nu3110910
Chimienti F, Devergnas S, Favier A, Seve M (2004) Diabetes 53:2330–2337
Andrews GK (2008) Biochem Soc Trans 36:1242–1246. doi:10.1042/BST0361242
Weaver BP, Dufner-Beattie J, Kambe T, Andrews GK (2007) Biol Chem 388:1301–1312. doi:10.1515/BC.2007.149
Girijashanker K, He L, Soleimani M, Reed JM, Li H, Liu Z, Wang B, Dalton TP, Nebert DW (2008) Mol Pharmacol 73:1413–1423. doi:10.1124/mol.107.043588
He L, Girijashanker K, Dalton TP, Reed J, Li H, Soleimani M, Nebert DW (2006) Mol Pharmacol 70:171–180. doi:10.1124/mol.106.024521
Hoch E, Lin W, Chai J, Hershfinkel M, Fu D, Sekler I (2012) Proc Natl Acad Sci USA 109:7202–7207. doi:10.1073/pnas.1200362109
Lye JC, Richards CD, Dechen K, Paterson D, de Jonge MD, Howard DL, Warr CG, Burke R (2012) J Exp Biol. doi:10.1242/jeb.069260
Van Doren M, Mathews WR, Samuels M, Moore LA, Broihier HT, Lehmann R (2003) Development 130:2355–2364
Mathews WR, Ong D, Milutinovich AB, Van Doren M (2006) Development 133:1143–1153
Stathakis DG, Burton DY, McIvor WE, Krishnakumar S, Wright TR, O’Donnell JM (1999) Genetics 153:361–382
Hsouna A, Lawal HO, Izevbaye I, Hsu T, O’Donnell JM (2007) Dev Biol 308:30–43. doi:10.1016/j.ydbio.2007.04.047
Yepiskoposyan H, Egli D, Fergestad T, Selvaraj A, Treiber C, Multhaup G, Georgiev O, Schaffner W (2006) Nucleic Acids Res 34:4866–4877
Wang X, Wu Y, Zhou B (2009) FASEB J 23:2650–2661
Georgiev P, Okkenhaug H, Drews A, Wright D, Lambert S, Flick M, Carta V, Martel C, Oberwinkler J, Raghu P (2010) Cell Metab 12:386–397
Dietzl G, Chen D, Schnorrer F, Su KC, Barinova Y, Fellner M, Gasser B, Kinsey K, Oppel S, Scheiblauer S, Couto A, Marra V, Keleman K, Dickson BJ (2007) Nature 448:151–156
Ralph DM, Robinson SR, Campbell MS, Bishop GM (2010) Free Radic Biol Med 49:649–657
Acknowledgments
Drosophila stocks were imported into Australia by the Australian Drosophila Biomedical Research Support Facility (http://www.ozdros.com). All Drosophila RNAi lines were provided by the Vienna Drosophila RNAi Center. Confocal microscopy was done at Monash Micro Imaging, which also provided training and technical support. This research was supported by a project grant (grant number 606609) from the Australian National Health and Medical Research Council.
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Lye, J.C., Richards, C.D., Dechen, K. et al. In vivo zinc toxicity phenotypes provide a sensitized background that suggests zinc transport activities for most of the Drosophila Zip and ZnT genes. J Biol Inorg Chem 18, 323–332 (2013). https://doi.org/10.1007/s00775-013-0976-6
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DOI: https://doi.org/10.1007/s00775-013-0976-6