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Structural evidence for a proton transfer pathway coupled with haem reduction of cytochrome c″ from Methylophilus methylotrophus

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Abstract

The crystal structures of the oxidized and reduced forms of cytochrome c″ from Methylophilus methylotrophus were solved from X-ray synchrotron data to atomic resolution. The overall fold of the molecule in the two redox states is very similar and is comparable to that of the oxygen-binding protein from the purple phototrophic bacterium Rhodobacter sphaeroides. However, significant modifications occur near the haem group, in particular the detachment from axial binding of His95 observed upon reduction as well as the adoption of different conformations of some protonatable residues that form a possible proton path from the haem pocket to the protein surface. These changes are associated with the previously well characterized redox-Bohr behaviour of this protein. Furthermore they provide a model for one of the presently proposed mechanisms of proton translocation in the much more complex protein cytochrome c oxidase.

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Acknowledgement

Access to the European Molecular Biology Laboratory–Deutsche Elektronen Synchrotron (EMBL-DESY) Hamburg Facility was supported through the European Commission program “Access to Research Infrastructure Action of the Improving Human Potential” grant HPRI-1999-CT-00017.

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Author notes

  1. Ehmke Pohl

    Present address: Swiss Light Source, Paul Scherrer Institute, 5232, Villigen PSI, Switzerland

Authors and Affiliations

  1. Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, P.O. Box 127, 2781-901, Oeiras, Portugal

    Francisco J. Enguita, Helena Santos & Maria Arménia Carrondo

  2. EMBL Hamburg Outstation, Building 25A, DESY, Notkestrasse 85, 22603, Hamburg, Germany

    Ehmke Pohl

  3. School of Chemistry, University of Southampton, Highfield, Southampton, SO17 1BJ, UK

    David L. Turner

Authors
  1. Francisco J. Enguita
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  2. Ehmke Pohl
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  3. David L. Turner
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  4. Helena Santos
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  5. Maria Arménia Carrondo
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Correspondence to Maria Arménia Carrondo.

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Enguita, F.J., Pohl, E., Turner, D.L. et al. Structural evidence for a proton transfer pathway coupled with haem reduction of cytochrome c″ from Methylophilus methylotrophus . J Biol Inorg Chem 11, 189–196 (2006). https://doi.org/10.1007/s00775-005-0065-6

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  • DOI: https://doi.org/10.1007/s00775-005-0065-6

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