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MiR-5100 promotes osteogenic differentiation by targeting Tob2

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Abstract

MicroRNAs have emerged as pivotal regulators in various physiological and pathological processes, including osteogenesis. Here we discuss the contribution of miR-5100 to osteoblast differentiation and mineralization. We found that miR-5100 was upregulated during osteoblast differentiation in ST2 and MC3T3-E1 cells. Next, we verified that miR-5100 can promote osteogenic differentiation with gain-of-function and loss-of-function experiments. Target prediction analysis and experimental validation demonstrated that Tob2, which acts as a negative regulator of osteogenesis, was negatively regulated by miR-5100. Furthermore, we confirmed that the important bone-related transcription factor osterix, which can be degraded by binding to Tob2, was influenced by miR-5100 during osteoblast differentiation. Collectively, our results revealed a new molecular mechanism that fine-tunes osteoblast differentiation through miR-5100/Tob2/osterix networks.

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Acknowledgements

This study was supported by the National Natural Science Foundation of China (81371909) and the Key Projects in the National Science and Technology Pillar Program during the Twelfth Five-Year Plan period (2013BAI07B01).

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Authors and Affiliations

  1. Shandong University of Traditional Chinese Medicine, Jinan, Shandong, China

    Huaxin Wang

  2. Shandong Medical Biotechnological Center, Key Laboratory for Biotech Drugs of the Ministry of Health, Shandong Academy of Medical Sciences, Jinan, China

    Huaxin Wang, Yazhou Cui, Jing Luan, Xiaoyan Zhou, Chengzhi Li, Haiying Li, Liang Shi & Jinxiang Han

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  1. Huaxin Wang
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  2. Yazhou Cui
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  3. Jing Luan
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Correspondence to Jinxiang Han.

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Wang, H., Cui, Y., Luan, J. et al. MiR-5100 promotes osteogenic differentiation by targeting Tob2. J Bone Miner Metab 35, 608–615 (2017). https://doi.org/10.1007/s00774-016-0799-y

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  • DOI: https://doi.org/10.1007/s00774-016-0799-y

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