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A validated high-performance thin-layer chromatography method for the determination of two bioactive lignans, phyllanthin and hypophyllanthin, in the seasonal variation study of Phyllanthus amarus

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Abstract

Phyllanthus amarus Schum. & Thonn. belongs to the family Euphorbiaceae, commonly known as “Bhooamalakee” or “Bhuti”, traditionally being used for the treatment of gastrointestinal troubles, kidney, liver problems, etc. In the present study, a validated high-performance thin-layer chromatography method was established for the estimation of two bioactive lignans, phyllanthin and hypophyllanthin, in the ethanolic extracts of P. amarus with the aim to see the effect of seasons on the quantity of the aforesaid phytoconstituents. The plant material was collected in July, August, September and October months during 2017–2018 from Lucknow, India. The method was validated in terms of precision, repeatability, specificity, sensitivity linearity and robustness. The method permits reliable quantification and showed good resolution on silica gel with toluene‒ethyl acetate (8.5:1.5, V/V) as the mobile phase, and characteristic bands of phyllanthin and hypophyllanthin were observed at RF 0.21, and 0.35, respectively. The level of quantified lignans varies in different months. Our finding indicated that the month of August may be appropriate for the collection of P. amarus for the preparation of therapeutic formulations because of the optimal level of both phyllanthin and hypophyllanthin, known for together work in the treatment of liver necrosis, carcinomas and asthma, etc.

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Acknowledgements

The authors are thankful to the Director, CSIR-National Botanical Research Institute, Lucknow for providing facilities.

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Authors and Affiliations

  1. Pharmacognosy Division, CSIR-National Botanical Research Institute, Post Box No. 436, Rana Pratap Marg, Lucknow, 226001, India

    Sayyada Khatoon & Saba Irshad

  2. Lucknow, 226016, India

    Sayyada Khatoon

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  1. Sayyada Khatoon
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  2. Saba Irshad
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Contributions

S. Khatoon: Study concept and experimental design, HPTLC densitometric analysis, analysis of data and manuscript writing and editing. S. Irshad: Literature search, statistical analysis of data and manuscript preparation.

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Correspondence to Sayyada Khatoon.

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Khatoon, S., Irshad, S. A validated high-performance thin-layer chromatography method for the determination of two bioactive lignans, phyllanthin and hypophyllanthin, in the seasonal variation study of Phyllanthus amarus. JPC-J Planar Chromat 34, 427–435 (2021). https://doi.org/10.1007/s00764-021-00129-1

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