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Alteration of metabolomic markers of amino-acid metabolism in piglets with in-feed antibiotics

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Abstract

In-feed antibiotics have been used to promote growth in piglets, but its impact on metabolomics profiles associated with host metabolism is largely unknown. In this study, to test the hypothesis that antibiotic treatment may affect metabolite composition both in the gut and host biofluids, metabolomics profiles were analyzed in antibiotic-treated piglets. Piglets were fed a corn-soy basal diet with or without in-feed antibiotics from postnatal day 7 to day 42. The serum biochemical parameters, metabolomics profiles of the serum, urine, and jejunal digesta, and indicators of microbial metabolism (short-chain fatty acids and biogenic amines) were analyzed. Compared to the control group, antibiotics treatment did not have significant effects on serum biochemical parameters except that it increased (P < 0.05) the concentration of urea. Antibiotics treatment increased the relative concentrations of metabolites involved in amino-acid metabolism in the serum, while decreased the relative concentrations of most amino acids in the jejunal content. Antibiotics reduced urinary 2-ketoisocaproate and hippurate. Furthermore, antibiotics decreased (P < 0.05) the concentrations of propionate and butyrate in the feces. Antibiotics significantly affected the concentrations of biogenic amines, which are derived from microbial amino-acid metabolism. The three major amines, putrescine, cadaverine, and spermidine, were all increased (P < 0.05) in the large intestine of antibiotics-treated piglets. These results identified the phenomena that in-feed antibiotics may have significant impact on the metabolomic markers of amino-acid metabolism in piglets.

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Acknowledgements

This work was supported by the National Key Basic Research Program of China (2013CB127300) and Natural Science Foundation of China (31430082). W. Zhu also thanks Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control for support.

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Authors and Affiliations

  1. Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal Health, Laboratory of Gastrointestinal Microbiology, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China

    Chunlong Mu, Yuxiang Yang, Kaifan Yu, Miao Yu, Chuanjian Zhang, Yong Su & Weiyun Zhu

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  1. Chunlong Mu
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  2. Yuxiang Yang
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  3. Kaifan Yu
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  4. Miao Yu
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  7. Weiyun Zhu
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Correspondence to Weiyun Zhu.

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All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. All procedures performed in studies involving animals were in accordance with the ethical standards of the institution or practice at which the studies were conducted. This article does not contain any studies with human participants performed by any of the authors.

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Handling Editor: C.-A. A. Hu.

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Mu, C., Yang, Y., Yu, K. et al. Alteration of metabolomic markers of amino-acid metabolism in piglets with in-feed antibiotics. Amino Acids 49, 771–781 (2017). https://doi.org/10.1007/s00726-017-2379-4

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  • DOI: https://doi.org/10.1007/s00726-017-2379-4

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