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Taurine chloramine modulates the expression of adipokines through inhibition of the STAT-3 signaling pathway in differentiated human adipocytes

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Abstract

To examine the possible role of taurine chloramine (TauCl) in modulating the expression of adipokines in adipose tissue associated with obesity, we evaluated the effect of TauCl in human differentiated adipocytes in response to IL-1β. To study the physiological effects of TauCl on adipokine expression, differentiated adipocytes were treated with IL-1β in the presence or absence of TauCl at concentrations ranging from 200 to 600 μM for 7 days. Cell culture supernatants and total RNA were analyzed by ELISA and real-time PCR, respectively, to determine protein and mRNA levels of adipokines, including adiponectin, leptin, IL-6, and IL-8. Levels of proteins involved in relevant signaling pathways were investigated by western blotting. Stimulation with IL-1β significantly decreased levels of adiponectin and leptin in adipocytes, but increased levels of IL-6 and IL-8 in a dose-dependent manner. Treatment with TauCl significantly reversed the modulation of adipokine expression by inhibiting STAT-3 signaling in IL-1β-stimulated adipocytes, independent of MAPK signaling. TauCl treatment more significantly modulated the expression of adipokines in adipocytes stimulated with IL-1β than that of non-stimulated adipocytes, suggesting that TauCl plays a significant role in modulating the expression of adipokines under inflammatory conditions. In conclusion, TauCl and other taurine derivatives that inhibit the STAT-3 signaling pathway can modulate expression of adipokines and thus may be useful as therapeutic agents for obesity-related diseases.

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Acknowledgments

This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF), which is funded by the Ministry of Education, Science and Technology (grant numbers 2010-0024089 and 2011-0009061).

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The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. East-West Bone and Joint Research Institute, Kyung Hee University Hospital at Gangdong, Kyung Hee University, Seoul, 134-727, Republic of Korea

    Kyoung Soo Kim, Hye-In Ji & Myung Chul Yoo

  2. Division of Rheumatology, Department of Internal Medicine, College of Medicine, Kyung Hee University, Seoul, Republic of Korea

    Sang Hoon Lee, Yeon-Ah Lee, Hyung-In Yang & Seung Jae Hong

  3. College of Medicine, Core Research Laboratory Clinical Research Institute, Kyung Hee University Hospital at Gangdong, Kyung Hee University, Seoul, 134-727, Republic of Korea

    Hyunju Chung

  4. Department of Pharmacology and BK21 Program, School of Medicine, Laboratory for Leukocyte Signaling Research, Inha University, Incheon, 400-712, Republic of Korea

    Chakyeun Kim

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  1. Kyoung Soo Kim
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  2. Hye-In Ji
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  3. Hyunju Chung
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  4. Chakyeun Kim
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  5. Sang Hoon Lee
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  8. Myung Chul Yoo
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  9. Seung Jae Hong
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Correspondence to Kyoung Soo Kim.

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Kim, K.S., Ji, HI., Chung, H. et al. Taurine chloramine modulates the expression of adipokines through inhibition of the STAT-3 signaling pathway in differentiated human adipocytes. Amino Acids 45, 1415–1422 (2013). https://doi.org/10.1007/s00726-013-1612-z

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  • DOI: https://doi.org/10.1007/s00726-013-1612-z

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