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Differential effects of cold, osmotic stress and abscisic acid on polyamine accumulation in wheat

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Abstract

The effects of cold, osmotic stress and abscisic acid (ABA) on polyamine accumulation were compared in the moderately freezing-sensitive wheat (Triticum aestivum L.) variety Chinese Spring (CS) and in two derived chromosome 5A substitution lines, CS(T. spelta 5A) and CS(Cheyenne 5A), exhibiting lower and higher levels of freezing tolerance, respectively. When compared with the other treatments, putrescine (Put) and spermidine (Spd) levels were much greater after cold treatment, spermine (Spm) following polyethylene glycol-induced (PEG) osmotic stress and Spm and cadaverine (Cad) after ABA treatment. During 3-week cold stress, the Put concentration, first exhibited a transient increase and decrease, and then gradually increased. These alterations may be due to changes in the expression of genes encoding the enzymes of Put synthesis. The Put content was higher in the freezing-tolerant chromosome 5A substitution line than in the sensitive one after 3 weeks of cold. In contrast to cold, ABA and PEG induced a continuous decrease in the Spd level when applied for a period of 3 weeks. The Spm content, which increased after PEG and ABA addition, was twice as high as that of Put during ABA treatment at most sampling points, but this difference was lower in the case of PEG. The Cad level, induced to a great extent by ABA, was much lower when compared with that of the other polyamines. The present experiments indicate that cold, osmotic stress and ABA have different effects on polyamines, and that some of these changes are affected by chromosome 5A and correlate with the level of stress tolerance.

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Abbreviations

ABA:

Abscisic acid

ADC:

Arginine decarboxylase

Agm:

Agmatine

Cad:

Cadaverine

cDNA:

Complementary deoxynucleic acid

CS:

Triticum aestivum cv. Chinese Spring

CS(Ch5A):

Chinese Spring (Cheyenne 5A) chromosome 5A substitution line

CS(Tsp5A):

Chinese Spring (T. spelta 5A) chromosome 5A substitution line

DAO:

Diamine oxidase

dNTP:

Deoxyribonucleotide triphosphate

DREB/CBF:

Drought responsive element-binding factor/C-repeat-binding factor

DTT:

Dithiothreitol

Fr-A1 and Fr-A2:

Freezing tolerance A1 and A2 locus

FW:

Fresh weight

GAPDH:

Glyceraldehyde-3-phosphate dehydrogenase

LDC:

Lysine decarboxylase

M-MLV:

Moloney-murine leukemia virus

ODC:

Ornithine decarboxylase

oligodT23 :

23mer Oligonucleotide dT

PAO:

Polyamine oxidase

PCR:

Polymerase chain reaction

PEG:

Polyethylene glycol

Put:

Putrescine

RNA:

Ribonucleic acid

RT:

Reverse transcriptase

SAM:

S-Adenosylmethionine

SAMDC:

S-Adenosylmethionine decarboxylase

Spd:

Spermidine

Spm:

Spermine

SSAT:

Spermidine/spermine N 1-acetyltransferase

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Acknowledgments

The authors wish to thank A. Horváth and N. Csabai for their help in plant cultivation and treatment, K. Hetes-Lőrincz for technical work in the analysis of polyamines, and C. Sovány and K. Kirsch for their help in gene expression studies. This work was supported by the German–Hungarian bilateral cooperation “PlantResource” (WTZ HUN 02/001), by the European Union (AGRISAFE 203288–EU-FP7-REGPOT2007-1) and by the Hungarian Scientific Research Fund and the National Office for Research and Technology (NKTH-OTKA K 67906).

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Authors and Affiliations

  1. Department of Applied Biotechnology and Food Science, Budapest University of Technology and Economics, P.O.B. 91, 1521, Budapest, Hungary

    Zita Kovács & Livia Simon-Sarkadi

  2. Agricultural Research Institute of the Hungarian Academy of Sciences, P.O.B. 19, 2462, Martonvásár, Hungary

    Attila Szűcs & Gábor Kocsy

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  1. Zita Kovács
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  2. Livia Simon-Sarkadi
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  4. Gábor Kocsy
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Correspondence to Livia Simon-Sarkadi.

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Kovács, Z., Simon-Sarkadi, L., Szűcs, A. et al. Differential effects of cold, osmotic stress and abscisic acid on polyamine accumulation in wheat. Amino Acids 38, 623–631 (2010). https://doi.org/10.1007/s00726-009-0423-8

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  • DOI: https://doi.org/10.1007/s00726-009-0423-8

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