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Gas chromatographic analysis of amino acid enantiomers in Carbetocin peptide hydrolysates after fast derivatization with pentafluoropropyl chloroformate

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Summary.

A novel sample preparation protocol for gas chromatographic (GC) analysis of amino acid enantiomers in peptides was developed. It comprises traditional acid hydrolysis, a novel treatment of the analytes with a fluoroalkyl chloroformate and GC/FID separation of enantiomers on a chiral capillary column. The major improvements consist in that the derivatization step proceeds in organic-aqueous media within seconds and the amino acid derivatives are volatile enough to suit the temperature range of the chiral Chirasil-Val® capillary column. The approach was found beneficial for chiral analysis of pharmaceutically important Carbetocin peptide.

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Authors and Affiliations

  1. Biology Centre, Academy of Sciences of the Czech Republic, Laboratory of Analytical Biochemistry, České Budějovice, Czech Republic

    H. Zahradníčková, P. Hartvich, P. Šimek & P. Hušek

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  1. H. Zahradníčková
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  2. P. Hartvich
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  3. P. Šimek
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  4. P. Hušek
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Authors’ address: Helena Zahradníčková, Biology Centre, Academy of Sciences of the Czech Republic, Laboratory of Analytical Biochemistry, Branišovská 31, 370 05 České Budějovice, Czech Republic

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Zahradníčková, H., Hartvich, P., Šimek, P. et al. Gas chromatographic analysis of amino acid enantiomers in Carbetocin peptide hydrolysates after fast derivatization with pentafluoropropyl chloroformate. Amino Acids 35, 445–450 (2008). https://doi.org/10.1007/s00726-007-0577-1

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  • DOI: https://doi.org/10.1007/s00726-007-0577-1

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