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Mass spectrometric analysis of protein histidine phosphorylation

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Summary.

Protein histidine phosphorylation is now recognized as an important form of post-translational modification. The acid-lability of phosphohistidine has meant that this phosphorylation has not been as well studied as serine/threonine or tyrosine phosphorylation. We show that phosphohistidine and phosphohistidine-containing phosphopeptides derived from proteolytic digestion of phosphohistone H4 are detectable by ESI-MS. We also demonstrate reverse-phase HPLC separation of these phosphopeptides and their detection by MALDI-TOF-MS.

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Authors and Affiliations

  1. School of Biomedical, Biomolecular and Chemical Sciences (M310), The University of Western Australia, Crawley, WA, Australia

    X.-L. Zu, P. G. Besant & P. V. Attwood

  2. Department of Molecular Biology, Adolf-Butenandt Institute, Histone Modifications Group, Ludwig-Maximilians-University of Munich, Munich, Germany

    A. Imhof

Authors
  1. X.-L. Zu
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  2. P. G. Besant
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  3. A. Imhof
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  4. P. V. Attwood
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Zu, XL., Besant, P., Imhof, A. et al. Mass spectrometric analysis of protein histidine phosphorylation. Amino Acids 32, 347–357 (2007). https://doi.org/10.1007/s00726-007-0493-4

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  • DOI: https://doi.org/10.1007/s00726-007-0493-4

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